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Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells
Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usua...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991571/ https://www.ncbi.nlm.nih.gov/pubmed/24747350 http://dx.doi.org/10.1371/journal.pone.0092999 |
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author | de Ghellinck, Alexis Schaller, Hubert Laux, Valérie Haertlein, Michael Sferrazza, Michele Maréchal, Eric Wacklin, Hanna Jouhet, Juliette Fragneto, Giovanna |
author_facet | de Ghellinck, Alexis Schaller, Hubert Laux, Valérie Haertlein, Michael Sferrazza, Michele Maréchal, Eric Wacklin, Hanna Jouhet, Juliette Fragneto, Giovanna |
author_sort | de Ghellinck, Alexis |
collection | PubMed |
description | Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture medium. Our results confirm that the production of well defined natural unsaturated perdeuterated lipids is possible and gives also new insights about the process of desaturase enzymes. |
format | Online Article Text |
id | pubmed-3991571 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-39915712014-04-21 Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells de Ghellinck, Alexis Schaller, Hubert Laux, Valérie Haertlein, Michael Sferrazza, Michele Maréchal, Eric Wacklin, Hanna Jouhet, Juliette Fragneto, Giovanna PLoS One Research Article Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture medium. Our results confirm that the production of well defined natural unsaturated perdeuterated lipids is possible and gives also new insights about the process of desaturase enzymes. Public Library of Science 2014-04-18 /pmc/articles/PMC3991571/ /pubmed/24747350 http://dx.doi.org/10.1371/journal.pone.0092999 Text en © 2014 de Ghellinck et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article de Ghellinck, Alexis Schaller, Hubert Laux, Valérie Haertlein, Michael Sferrazza, Michele Maréchal, Eric Wacklin, Hanna Jouhet, Juliette Fragneto, Giovanna Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells |
title | Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells |
title_full | Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells |
title_fullStr | Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells |
title_full_unstemmed | Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells |
title_short | Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells |
title_sort | production and analysis of perdeuterated lipids from pichia pastoris cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991571/ https://www.ncbi.nlm.nih.gov/pubmed/24747350 http://dx.doi.org/10.1371/journal.pone.0092999 |
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