Cargando…

Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells

Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usua...

Descripción completa

Detalles Bibliográficos
Autores principales: de Ghellinck, Alexis, Schaller, Hubert, Laux, Valérie, Haertlein, Michael, Sferrazza, Michele, Maréchal, Eric, Wacklin, Hanna, Jouhet, Juliette, Fragneto, Giovanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991571/
https://www.ncbi.nlm.nih.gov/pubmed/24747350
http://dx.doi.org/10.1371/journal.pone.0092999
_version_ 1782312459347951616
author de Ghellinck, Alexis
Schaller, Hubert
Laux, Valérie
Haertlein, Michael
Sferrazza, Michele
Maréchal, Eric
Wacklin, Hanna
Jouhet, Juliette
Fragneto, Giovanna
author_facet de Ghellinck, Alexis
Schaller, Hubert
Laux, Valérie
Haertlein, Michael
Sferrazza, Michele
Maréchal, Eric
Wacklin, Hanna
Jouhet, Juliette
Fragneto, Giovanna
author_sort de Ghellinck, Alexis
collection PubMed
description Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture medium. Our results confirm that the production of well defined natural unsaturated perdeuterated lipids is possible and gives also new insights about the process of desaturase enzymes.
format Online
Article
Text
id pubmed-3991571
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-39915712014-04-21 Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells de Ghellinck, Alexis Schaller, Hubert Laux, Valérie Haertlein, Michael Sferrazza, Michele Maréchal, Eric Wacklin, Hanna Jouhet, Juliette Fragneto, Giovanna PLoS One Research Article Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture medium. Our results confirm that the production of well defined natural unsaturated perdeuterated lipids is possible and gives also new insights about the process of desaturase enzymes. Public Library of Science 2014-04-18 /pmc/articles/PMC3991571/ /pubmed/24747350 http://dx.doi.org/10.1371/journal.pone.0092999 Text en © 2014 de Ghellinck et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
de Ghellinck, Alexis
Schaller, Hubert
Laux, Valérie
Haertlein, Michael
Sferrazza, Michele
Maréchal, Eric
Wacklin, Hanna
Jouhet, Juliette
Fragneto, Giovanna
Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells
title Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells
title_full Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells
title_fullStr Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells
title_full_unstemmed Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells
title_short Production and Analysis of Perdeuterated Lipids from Pichia pastoris Cells
title_sort production and analysis of perdeuterated lipids from pichia pastoris cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991571/
https://www.ncbi.nlm.nih.gov/pubmed/24747350
http://dx.doi.org/10.1371/journal.pone.0092999
work_keys_str_mv AT deghellinckalexis productionandanalysisofperdeuteratedlipidsfrompichiapastoriscells
AT schallerhubert productionandanalysisofperdeuteratedlipidsfrompichiapastoriscells
AT lauxvalerie productionandanalysisofperdeuteratedlipidsfrompichiapastoriscells
AT haertleinmichael productionandanalysisofperdeuteratedlipidsfrompichiapastoriscells
AT sferrazzamichele productionandanalysisofperdeuteratedlipidsfrompichiapastoriscells
AT marechaleric productionandanalysisofperdeuteratedlipidsfrompichiapastoriscells
AT wacklinhanna productionandanalysisofperdeuteratedlipidsfrompichiapastoriscells
AT jouhetjuliette productionandanalysisofperdeuteratedlipidsfrompichiapastoriscells
AT fragnetogiovanna productionandanalysisofperdeuteratedlipidsfrompichiapastoriscells