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Variation in the Degree of Pectin Methylesterification during the Development of Baccharis dracunculifolia Kidney-Shaped Gall

Insect galls may be study models to test the distribution of pectins and arabinogalactan-proteins (AGPs) and their related functions during plant cell cycles. These molecules are herein histochemically and immunocitochemically investigated in the kidney-shaped gall induced by Baccharopelma dracuncul...

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Autores principales: de Oliveira, Denis Coelho, Magalhães, Thiago Alves, Ferreira, Bruno Garcia, Teixeira, Cristiane Trindade, Formiga, Anete Teixeira, Fernandes, Geraldo Wilson, Isaias, Rosy Mary dos Santos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991646/
https://www.ncbi.nlm.nih.gov/pubmed/24747777
http://dx.doi.org/10.1371/journal.pone.0094588
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author de Oliveira, Denis Coelho
Magalhães, Thiago Alves
Ferreira, Bruno Garcia
Teixeira, Cristiane Trindade
Formiga, Anete Teixeira
Fernandes, Geraldo Wilson
Isaias, Rosy Mary dos Santos
author_facet de Oliveira, Denis Coelho
Magalhães, Thiago Alves
Ferreira, Bruno Garcia
Teixeira, Cristiane Trindade
Formiga, Anete Teixeira
Fernandes, Geraldo Wilson
Isaias, Rosy Mary dos Santos
author_sort de Oliveira, Denis Coelho
collection PubMed
description Insect galls may be study models to test the distribution of pectins and arabinogalactan-proteins (AGPs) and their related functions during plant cell cycles. These molecules are herein histochemically and immunocitochemically investigated in the kidney-shaped gall induced by Baccharopelma dracunculifoliae (Psyllidae) on leaves of Baccharis dracunculifolia DC. (Asteraceae) on developmental basis. The homogalacturonans (HGAs) (labeled by JIM5) and the arabinans (labeled by LM6) were detected either in non-galled leaves or in young galls, and indicated stiffening of epidermal cell walls, which is an important step for cell redifferentiation. The labeling of HGAs by JIM7 changed from young to senescent stage, with an increase in the rigidity of cell walls, which is important for the acquaintance of the final gall shape and for the mechanical opening of the gall. The variation on the degree of HGAs during gall development indicated differential PMEs activity during gall development. The epitopes recognized by LM2 (AGP glycan) and LM5 (1–4-β-D-galactans) had poor alterations from non-galled leaves towards gall maturation and senescence. Moreover, the dynamics of pectin and AGPs on two comparable mature kidney-shaped galls on B. dracunculifolia and on B. reticularia revealed specific peculiarities. Our results indicate that similar gall morphotypes in cogeneric host species may present distinct cell responses in the subcelular level, and also corroborate the functions proposed in literature for HGAs.
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spelling pubmed-39916462014-04-21 Variation in the Degree of Pectin Methylesterification during the Development of Baccharis dracunculifolia Kidney-Shaped Gall de Oliveira, Denis Coelho Magalhães, Thiago Alves Ferreira, Bruno Garcia Teixeira, Cristiane Trindade Formiga, Anete Teixeira Fernandes, Geraldo Wilson Isaias, Rosy Mary dos Santos PLoS One Research Article Insect galls may be study models to test the distribution of pectins and arabinogalactan-proteins (AGPs) and their related functions during plant cell cycles. These molecules are herein histochemically and immunocitochemically investigated in the kidney-shaped gall induced by Baccharopelma dracunculifoliae (Psyllidae) on leaves of Baccharis dracunculifolia DC. (Asteraceae) on developmental basis. The homogalacturonans (HGAs) (labeled by JIM5) and the arabinans (labeled by LM6) were detected either in non-galled leaves or in young galls, and indicated stiffening of epidermal cell walls, which is an important step for cell redifferentiation. The labeling of HGAs by JIM7 changed from young to senescent stage, with an increase in the rigidity of cell walls, which is important for the acquaintance of the final gall shape and for the mechanical opening of the gall. The variation on the degree of HGAs during gall development indicated differential PMEs activity during gall development. The epitopes recognized by LM2 (AGP glycan) and LM5 (1–4-β-D-galactans) had poor alterations from non-galled leaves towards gall maturation and senescence. Moreover, the dynamics of pectin and AGPs on two comparable mature kidney-shaped galls on B. dracunculifolia and on B. reticularia revealed specific peculiarities. Our results indicate that similar gall morphotypes in cogeneric host species may present distinct cell responses in the subcelular level, and also corroborate the functions proposed in literature for HGAs. Public Library of Science 2014-04-18 /pmc/articles/PMC3991646/ /pubmed/24747777 http://dx.doi.org/10.1371/journal.pone.0094588 Text en © 2014 de Oliveira et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
de Oliveira, Denis Coelho
Magalhães, Thiago Alves
Ferreira, Bruno Garcia
Teixeira, Cristiane Trindade
Formiga, Anete Teixeira
Fernandes, Geraldo Wilson
Isaias, Rosy Mary dos Santos
Variation in the Degree of Pectin Methylesterification during the Development of Baccharis dracunculifolia Kidney-Shaped Gall
title Variation in the Degree of Pectin Methylesterification during the Development of Baccharis dracunculifolia Kidney-Shaped Gall
title_full Variation in the Degree of Pectin Methylesterification during the Development of Baccharis dracunculifolia Kidney-Shaped Gall
title_fullStr Variation in the Degree of Pectin Methylesterification during the Development of Baccharis dracunculifolia Kidney-Shaped Gall
title_full_unstemmed Variation in the Degree of Pectin Methylesterification during the Development of Baccharis dracunculifolia Kidney-Shaped Gall
title_short Variation in the Degree of Pectin Methylesterification during the Development of Baccharis dracunculifolia Kidney-Shaped Gall
title_sort variation in the degree of pectin methylesterification during the development of baccharis dracunculifolia kidney-shaped gall
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3991646/
https://www.ncbi.nlm.nih.gov/pubmed/24747777
http://dx.doi.org/10.1371/journal.pone.0094588
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