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Transcriptional mechanisms of cell fate decisions revealed by single cell expression profiling
Transcriptional networks regulate cell fate decisions, which occur at the level of individual cells. However, much of what we know about their structure and function comes from studies averaging measurements over large populations of cells, many of which are functionally heterogeneous. Such studies...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
WILEY Periodicals, Inc.
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3992849/ https://www.ncbi.nlm.nih.gov/pubmed/24470343 http://dx.doi.org/10.1002/bies.201300102 |
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author | Moignard, Victoria Göttgens, Berthold |
author_facet | Moignard, Victoria Göttgens, Berthold |
author_sort | Moignard, Victoria |
collection | PubMed |
description | Transcriptional networks regulate cell fate decisions, which occur at the level of individual cells. However, much of what we know about their structure and function comes from studies averaging measurements over large populations of cells, many of which are functionally heterogeneous. Such studies conceal the variability between cells and so prevent us from determining the nature of heterogeneity at the molecular level. In recent years, many protocols and platforms have been developed that allow the high throughput analysis of gene expression in single cells, opening the door to a new era of biology. Here, we discuss the need for single cell gene expression analysis to gain deeper insights into the transcriptional control of cell fate decisions, and consider the insights it has provided so far into transcriptional regulatory networks in development. |
format | Online Article Text |
id | pubmed-3992849 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | WILEY Periodicals, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-39928492014-04-22 Transcriptional mechanisms of cell fate decisions revealed by single cell expression profiling Moignard, Victoria Göttgens, Berthold Bioessays Prospects & Overviews Transcriptional networks regulate cell fate decisions, which occur at the level of individual cells. However, much of what we know about their structure and function comes from studies averaging measurements over large populations of cells, many of which are functionally heterogeneous. Such studies conceal the variability between cells and so prevent us from determining the nature of heterogeneity at the molecular level. In recent years, many protocols and platforms have been developed that allow the high throughput analysis of gene expression in single cells, opening the door to a new era of biology. Here, we discuss the need for single cell gene expression analysis to gain deeper insights into the transcriptional control of cell fate decisions, and consider the insights it has provided so far into transcriptional regulatory networks in development. WILEY Periodicals, Inc. 2014-04 2014-01-28 /pmc/articles/PMC3992849/ /pubmed/24470343 http://dx.doi.org/10.1002/bies.201300102 Text en © 2014 The Authors. Bioessays published by WILEY Periodicals, Inc. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Prospects & Overviews Moignard, Victoria Göttgens, Berthold Transcriptional mechanisms of cell fate decisions revealed by single cell expression profiling |
title | Transcriptional mechanisms of cell fate decisions revealed by single cell expression profiling |
title_full | Transcriptional mechanisms of cell fate decisions revealed by single cell expression profiling |
title_fullStr | Transcriptional mechanisms of cell fate decisions revealed by single cell expression profiling |
title_full_unstemmed | Transcriptional mechanisms of cell fate decisions revealed by single cell expression profiling |
title_short | Transcriptional mechanisms of cell fate decisions revealed by single cell expression profiling |
title_sort | transcriptional mechanisms of cell fate decisions revealed by single cell expression profiling |
topic | Prospects & Overviews |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3992849/ https://www.ncbi.nlm.nih.gov/pubmed/24470343 http://dx.doi.org/10.1002/bies.201300102 |
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