Rational Domain Swaps Reveal Insights about Chain Length Control by Ketosynthase Domains in Fungal Nonreducing Polyketide Synthases

[Image: see text] A facile genetic methodology in the filamentous fungus Aspergillus nidulans allowed exchange of the starter unit ACP transacylase (SAT) domain in the nonreduced polyketide synthase (NR-PKS) AfoE of the asperfuranone pathway with the SAT domains from 10 other NR-PKSs. The newly crea...

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Autores principales: Liu, Ting, Sanchez, James F., Chiang, Yi-Ming, Oakley, Berl R., Wang, Clay C. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993715/
https://www.ncbi.nlm.nih.gov/pubmed/24593241
http://dx.doi.org/10.1021/ol5003384
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author Liu, Ting
Sanchez, James F.
Chiang, Yi-Ming
Oakley, Berl R.
Wang, Clay C. C.
author_facet Liu, Ting
Sanchez, James F.
Chiang, Yi-Ming
Oakley, Berl R.
Wang, Clay C. C.
author_sort Liu, Ting
collection PubMed
description [Image: see text] A facile genetic methodology in the filamentous fungus Aspergillus nidulans allowed exchange of the starter unit ACP transacylase (SAT) domain in the nonreduced polyketide synthase (NR-PKS) AfoE of the asperfuranone pathway with the SAT domains from 10 other NR-PKSs. The newly created hybrid with the NR-PKS AN3386 is able to accept a longer starter unit in place of the native substrate to create a novel aromatic polyketide in vivo.
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spelling pubmed-39937152015-03-05 Rational Domain Swaps Reveal Insights about Chain Length Control by Ketosynthase Domains in Fungal Nonreducing Polyketide Synthases Liu, Ting Sanchez, James F. Chiang, Yi-Ming Oakley, Berl R. Wang, Clay C. C. Org Lett [Image: see text] A facile genetic methodology in the filamentous fungus Aspergillus nidulans allowed exchange of the starter unit ACP transacylase (SAT) domain in the nonreduced polyketide synthase (NR-PKS) AfoE of the asperfuranone pathway with the SAT domains from 10 other NR-PKSs. The newly created hybrid with the NR-PKS AN3386 is able to accept a longer starter unit in place of the native substrate to create a novel aromatic polyketide in vivo. American Chemical Society 2014-03-05 2014-03-21 /pmc/articles/PMC3993715/ /pubmed/24593241 http://dx.doi.org/10.1021/ol5003384 Text en Copyright © 2014 American Chemical Society
spellingShingle Liu, Ting
Sanchez, James F.
Chiang, Yi-Ming
Oakley, Berl R.
Wang, Clay C. C.
Rational Domain Swaps Reveal Insights about Chain Length Control by Ketosynthase Domains in Fungal Nonreducing Polyketide Synthases
title Rational Domain Swaps Reveal Insights about Chain Length Control by Ketosynthase Domains in Fungal Nonreducing Polyketide Synthases
title_full Rational Domain Swaps Reveal Insights about Chain Length Control by Ketosynthase Domains in Fungal Nonreducing Polyketide Synthases
title_fullStr Rational Domain Swaps Reveal Insights about Chain Length Control by Ketosynthase Domains in Fungal Nonreducing Polyketide Synthases
title_full_unstemmed Rational Domain Swaps Reveal Insights about Chain Length Control by Ketosynthase Domains in Fungal Nonreducing Polyketide Synthases
title_short Rational Domain Swaps Reveal Insights about Chain Length Control by Ketosynthase Domains in Fungal Nonreducing Polyketide Synthases
title_sort rational domain swaps reveal insights about chain length control by ketosynthase domains in fungal nonreducing polyketide synthases
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993715/
https://www.ncbi.nlm.nih.gov/pubmed/24593241
http://dx.doi.org/10.1021/ol5003384
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