Cargando…
Interaction of Hyaluronan Binding Peptides with Glycosaminoglycans in Poly(ethylene glycol) Hydrogels
[Image: see text] This study investigates the incorporation of hyaluronan (HA) binding peptides into poly(ethylene glycol) (PEG) hydrogels as a mechanism to bind and retain hyaluronan for applications in tissue engineering. The specificity of the peptide sequence (native RYPISRPRKRC vs non-native RP...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2014
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993952/ https://www.ncbi.nlm.nih.gov/pubmed/24597474 http://dx.doi.org/10.1021/bm401524h |
_version_ | 1782312642992406528 |
---|---|
author | Roberts, Justine J. Elder, Robert M. Neumann, Alexander J. Jayaraman, Arthi Bryant, Stephanie J. |
author_facet | Roberts, Justine J. Elder, Robert M. Neumann, Alexander J. Jayaraman, Arthi Bryant, Stephanie J. |
author_sort | Roberts, Justine J. |
collection | PubMed |
description | [Image: see text] This study investigates the incorporation of hyaluronan (HA) binding peptides into poly(ethylene glycol) (PEG) hydrogels as a mechanism to bind and retain hyaluronan for applications in tissue engineering. The specificity of the peptide sequence (native RYPISRPRKRC vs non-native RPSRPRIRYKC), the role of basic amino acids, and specificity to hyaluronan over other GAGs in contributing to the peptide–hyaluronan interaction were probed through experiments and simulations. Hydrogels containing the native or non-native peptide retained hyaluronan in a dose-dependent manner. Ionic interactions were the dominating mechanism. In diH(2)O the peptides interacted strongly with HA and chondroitin sulfate, but in phosphate buffered saline the peptides interacted more strongly with HA. For cartilage tissue engineering, chondrocyte-laden PEG hydrogels containing increasing amounts of HA binding peptide and exogenous HA had increased retention and decreased loss of cell-secreted proteoglycans in and from the hydrogel at 28 days. This new matrix-interactive hydrogel platform holds promise for tissue regeneration. |
format | Online Article Text |
id | pubmed-3993952 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-39939522015-03-06 Interaction of Hyaluronan Binding Peptides with Glycosaminoglycans in Poly(ethylene glycol) Hydrogels Roberts, Justine J. Elder, Robert M. Neumann, Alexander J. Jayaraman, Arthi Bryant, Stephanie J. Biomacromolecules [Image: see text] This study investigates the incorporation of hyaluronan (HA) binding peptides into poly(ethylene glycol) (PEG) hydrogels as a mechanism to bind and retain hyaluronan for applications in tissue engineering. The specificity of the peptide sequence (native RYPISRPRKRC vs non-native RPSRPRIRYKC), the role of basic amino acids, and specificity to hyaluronan over other GAGs in contributing to the peptide–hyaluronan interaction were probed through experiments and simulations. Hydrogels containing the native or non-native peptide retained hyaluronan in a dose-dependent manner. Ionic interactions were the dominating mechanism. In diH(2)O the peptides interacted strongly with HA and chondroitin sulfate, but in phosphate buffered saline the peptides interacted more strongly with HA. For cartilage tissue engineering, chondrocyte-laden PEG hydrogels containing increasing amounts of HA binding peptide and exogenous HA had increased retention and decreased loss of cell-secreted proteoglycans in and from the hydrogel at 28 days. This new matrix-interactive hydrogel platform holds promise for tissue regeneration. American Chemical Society 2014-03-06 2014-04-14 /pmc/articles/PMC3993952/ /pubmed/24597474 http://dx.doi.org/10.1021/bm401524h Text en Copyright © 2014 American Chemical Society |
spellingShingle | Roberts, Justine J. Elder, Robert M. Neumann, Alexander J. Jayaraman, Arthi Bryant, Stephanie J. Interaction of Hyaluronan Binding Peptides with Glycosaminoglycans in Poly(ethylene glycol) Hydrogels |
title | Interaction of Hyaluronan Binding Peptides with Glycosaminoglycans
in Poly(ethylene glycol) Hydrogels |
title_full | Interaction of Hyaluronan Binding Peptides with Glycosaminoglycans
in Poly(ethylene glycol) Hydrogels |
title_fullStr | Interaction of Hyaluronan Binding Peptides with Glycosaminoglycans
in Poly(ethylene glycol) Hydrogels |
title_full_unstemmed | Interaction of Hyaluronan Binding Peptides with Glycosaminoglycans
in Poly(ethylene glycol) Hydrogels |
title_short | Interaction of Hyaluronan Binding Peptides with Glycosaminoglycans
in Poly(ethylene glycol) Hydrogels |
title_sort | interaction of hyaluronan binding peptides with glycosaminoglycans
in poly(ethylene glycol) hydrogels |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993952/ https://www.ncbi.nlm.nih.gov/pubmed/24597474 http://dx.doi.org/10.1021/bm401524h |
work_keys_str_mv | AT robertsjustinej interactionofhyaluronanbindingpeptideswithglycosaminoglycansinpolyethyleneglycolhydrogels AT elderrobertm interactionofhyaluronanbindingpeptideswithglycosaminoglycansinpolyethyleneglycolhydrogels AT neumannalexanderj interactionofhyaluronanbindingpeptideswithglycosaminoglycansinpolyethyleneglycolhydrogels AT jayaramanarthi interactionofhyaluronanbindingpeptideswithglycosaminoglycansinpolyethyleneglycolhydrogels AT bryantstephaniej interactionofhyaluronanbindingpeptideswithglycosaminoglycansinpolyethyleneglycolhydrogels |