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Alterations in the Rat Serum Proteome Induced by Prepubertal Exposure to Bisphenol A and Genistein
[Image: see text] Humans are exposed to an array of chemicals via the food, drink and air, including a significant number that can mimic endogenous hormones. One such chemical is Bisphenol A (BPA), a synthetic chemical that has been shown to cause developmental alterations and to predispose for mamm...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical
Society
2014
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993963/ https://www.ncbi.nlm.nih.gov/pubmed/24552547 http://dx.doi.org/10.1021/pr401027q |
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author | Betancourt, Angela Mobley, James A. Wang, Jun Jenkins, Sarah Chen, Dongquan Kojima, Kyoko Russo, Jose Lamartiniere, Coral A. |
author_facet | Betancourt, Angela Mobley, James A. Wang, Jun Jenkins, Sarah Chen, Dongquan Kojima, Kyoko Russo, Jose Lamartiniere, Coral A. |
author_sort | Betancourt, Angela |
collection | PubMed |
description | [Image: see text] Humans are exposed to an array of chemicals via the food, drink and air, including a significant number that can mimic endogenous hormones. One such chemical is Bisphenol A (BPA), a synthetic chemical that has been shown to cause developmental alterations and to predispose for mammary cancer in rodent models. In contrast, the phytochemical genistein has been reported to suppress chemically induced mammary cancer in rodents, and Asians ingesting a diet high in soy containing genistein have lower incidence of breast and prostate cancers. In this study, we sought to: (1) identify protein biomarkers of susceptibility from blood sera of rats exposed prepubertally to BPA or genistein using Isobaric Tandem Mass Tags quantitative mass spectrometry (TMT-MS) combined with MudPIT technology and, (2) explore the relevance of these proteins to carcinogenesis. Prepubertal exposures to BPA and genistein resulted in altered expression of 63 and 28 proteins in rat sera at postnatal day (PND) 21, and of 9 and 18 proteins in sera at PND35, respectively. This study demonstrates the value of using quantitative proteomic techniques to explore the effect of chemical exposure on the rat serum proteome and its potential for unraveling cellular targets altered by BPA and genistein involved in carcinogenesis. |
format | Online Article Text |
id | pubmed-3993963 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | American Chemical
Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-39939632015-02-05 Alterations in the Rat Serum Proteome Induced by Prepubertal Exposure to Bisphenol A and Genistein Betancourt, Angela Mobley, James A. Wang, Jun Jenkins, Sarah Chen, Dongquan Kojima, Kyoko Russo, Jose Lamartiniere, Coral A. J Proteome Res [Image: see text] Humans are exposed to an array of chemicals via the food, drink and air, including a significant number that can mimic endogenous hormones. One such chemical is Bisphenol A (BPA), a synthetic chemical that has been shown to cause developmental alterations and to predispose for mammary cancer in rodent models. In contrast, the phytochemical genistein has been reported to suppress chemically induced mammary cancer in rodents, and Asians ingesting a diet high in soy containing genistein have lower incidence of breast and prostate cancers. In this study, we sought to: (1) identify protein biomarkers of susceptibility from blood sera of rats exposed prepubertally to BPA or genistein using Isobaric Tandem Mass Tags quantitative mass spectrometry (TMT-MS) combined with MudPIT technology and, (2) explore the relevance of these proteins to carcinogenesis. Prepubertal exposures to BPA and genistein resulted in altered expression of 63 and 28 proteins in rat sera at postnatal day (PND) 21, and of 9 and 18 proteins in sera at PND35, respectively. This study demonstrates the value of using quantitative proteomic techniques to explore the effect of chemical exposure on the rat serum proteome and its potential for unraveling cellular targets altered by BPA and genistein involved in carcinogenesis. American Chemical Society 2014-02-05 2014-03-07 /pmc/articles/PMC3993963/ /pubmed/24552547 http://dx.doi.org/10.1021/pr401027q Text en Copyright © 2014 American Chemical Society |
spellingShingle | Betancourt, Angela Mobley, James A. Wang, Jun Jenkins, Sarah Chen, Dongquan Kojima, Kyoko Russo, Jose Lamartiniere, Coral A. Alterations in the Rat Serum Proteome Induced by Prepubertal Exposure to Bisphenol A and Genistein |
title | Alterations in the Rat Serum
Proteome Induced by Prepubertal
Exposure to Bisphenol A and Genistein |
title_full | Alterations in the Rat Serum
Proteome Induced by Prepubertal
Exposure to Bisphenol A and Genistein |
title_fullStr | Alterations in the Rat Serum
Proteome Induced by Prepubertal
Exposure to Bisphenol A and Genistein |
title_full_unstemmed | Alterations in the Rat Serum
Proteome Induced by Prepubertal
Exposure to Bisphenol A and Genistein |
title_short | Alterations in the Rat Serum
Proteome Induced by Prepubertal
Exposure to Bisphenol A and Genistein |
title_sort | alterations in the rat serum
proteome induced by prepubertal
exposure to bisphenol a and genistein |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993963/ https://www.ncbi.nlm.nih.gov/pubmed/24552547 http://dx.doi.org/10.1021/pr401027q |
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