Cargando…

Magnetic isolation of Plasmodium falciparum schizonts iRBCs to generate a high parasitaemia and synchronized in vitro culture

BACKGROUND: The establishment of methods for an in vitro continuous culture of Plasmodium falciparum is essential for gaining knowledge into its biology and for the development of new treatments. Previously, several techniques have been used to synchronize, enrich and concentrate P. falciparum, alth...

Descripción completa

Detalles Bibliográficos
Autores principales:	Mata-Cantero, Lydia, Lafuente, Maria J, Sanz, Laura, Rodriguez, Manuel S
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2014
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3994429/ https://www.ncbi.nlm.nih.gov/pubmed/24655321 http://dx.doi.org/10.1186/1475-2875-13-112

Ejemplares similares

Cryogenically preserved RBCs support gametocytogenesis of Plasmodium falciparum in vitro and gametogenesis in mosquitoes
por: Pathak, Ashutosh K., et al.
Publicado: (2018)

Comparison of the in vitro invasive capabilities of Plasmodium falciparum schizonts isolated by Percoll gradient or using magnetic based separation
por: Spadafora, Carmenza, et al.
Publicado: (2011)

Developing Gametocytes and Schizonts of Plasmodium Falciparum
Publicado: (1944)

Purification of native histidine-rich protein 2 (nHRP2) from Plasmodium falciparum culture supernatant, infected RBCs, and parasite lysate
por: Singh, Balwan, et al.
Publicado: (2021)

High-throughput tri-colour flow cytometry technique to assess Plasmodium falciparum parasitaemia in bioassays
por: Tiendrebeogo, Regis W, et al.
Publicado: (2014)

Optimization of parasite DNA enrichment approaches to generate whole genome sequencing data for Plasmodium falciparum from low parasitaemia samples
por: Shah, Zalak, et al.
Publicado: (2020)

New synchronization method for Plasmodium falciparum
por: Ranford-Cartwright, Lisa C, et al.
Publicado: (2010)

Plasmodium falciparum rosetting protects schizonts against artemisinin
por: Lee, Wenn-Chyau, et al.
Publicado: (2021)

Stochastic expression of invasion genes in Plasmodium falciparum schizonts
por: Tripathi, Jaishree, et al.
Publicado: (2022)

An alternative protocol for Plasmodium falciparum culture synchronization and a new method for synchrony confirmation
por: Childs, Ronnie A, et al.
Publicado: (2013)

Plasmodium falciparum Rab1A Localizes to Rhoptries in Schizonts
por: Morse, David, et al.
Publicado: (2016)

Determinants on surface proteins of Plasmodium knowlesi merozoites common to Plasmodium falciparum schizonts
Publicado: (1980)

A Plasmodium falciparum FcB1-schizont-EST collection providing clues to schizont specific gene structure and polymorphism
por: Florent, Isabelle, et al.
Publicado: (2009)

ARAM: an automated image analysis software to determine rosetting parameters and parasitaemia in Plasmodium samples
por: Kudella, Patrick Wolfgang, et al.
Publicado: (2016)

Ubiquitin activation is essential for schizont maturation in Plasmodium falciparum blood-stage development
por: Green, Judith L., et al.
Publicado: (2020)

Mid-Liver Stage Arrest of Plasmodium falciparum Schizonts in Primary Porcine Hepatocytes
por: van der Boor, Saskia C., et al.
Publicado: (2022)

Haemoglobin interference and increased sensitivity of fluorimetric assays for quantification of low-parasitaemia Plasmodium infected erythrocytes
por: Moneriz, Carlos, et al.
Publicado: (2009)

A method to preserve low parasitaemia Plasmodium-infected avian blood for host and vector infectivity assays
por: Carlson, Jenny S., et al.
Publicado: (2016)

Expression profiling of the schizont and trophozoite stages of Plasmodium falciparum with a long-oligonucleotide microarray
por: Bozdech, Zbynek, et al.
Publicado: (2003)

Efficient synchronization of Plasmodium knowlesi in vitro cultures using guanidine hydrochloride
por: Ngernna, Sutharinee, et al.
Publicado: (2019)

SURFIN(4.1), a schizont-merozoite associated protein in the SURFIN family of Plasmodium falciparum
por: Mphande, Fingani A, et al.
Publicado: (2008)

Polymorphism of a high molecular weight schizont antigen of the human malaria parasite Plasmodium falciparum
Publicado: (1985)

Developing Gametocytes and Schizonts of Plasmodium Falciparum: A Case Showing All Stages in the Peripheral Circulation
por: Gupta, B. M. Das, et al.
Publicado: (1944)

A regulatable transgene expression system for cultured Plasmodium falciparum parasites
por: Epp, Christian, et al.
Publicado: (2008)

Plate-based transfection and culturing technique for genetic manipulation of Plasmodium falciparum
por: Caro, Florence, et al.
Publicado: (2012)

Literacy and recent history of diarrhoea are predictive of Plasmodium falciparum parasitaemia in Kenyan adults
por: Coldren, Rodney L, et al.
Publicado: (2006)

Estimating the parasitaemia of Plasmodium falciparum: experience from a national EQA scheme
por: Manser, Monika, et al.
Publicado: (2013)

Biosynthesis and processing of a Plasmodium falciparum schizont antigen recognized by immune serum and a monoclonal antibody
Publicado: (1982)

Subcellular Location, Phosphorylation and Assembly into the Motor Complex of GAP45 during Plasmodium falciparum Schizont Development
por: Ridzuan, Mohd A. Mohd, et al.
Publicado: (2012)

Schizont transcriptome variation among clinical isolates and laboratory-adapted clones of the malaria parasite Plasmodium falciparum
por: Tarr, Sarah J., et al.
Publicado: (2018)

Purification and production of Plasmodium falciparum zygotes from in vitro culture using magnetic column and Percoll density gradient
por: Zhou, Yaxian, et al.
Publicado: (2020)

Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures
por: Ferrer, Jordi, et al.
Publicado: (2008)

Risk of Plasmodium vivax parasitaemia after Plasmodium falciparum infection: a systematic review and meta-analysis
por: Commons, Robert J, et al.
Publicado: (2019)

In vivo transcriptional profiling of Plasmodium falciparum
por: Daily, Johanna P, et al.
Publicado: (2004)

Laser-induced inactivation of Plasmodium falciparum
por: LeBlanc, Danielle, et al.
Publicado: (2012)

Comparison of Plasmodium falciparum transfection methods
por: Skinner-Adams, Tina S, et al.
Publicado: (2003)

Reduction in the proportion of fevers associated with Plasmodium falciparum parasitaemia in Africa: a systematic review
por: D'Acremont, Valérie, et al.
Publicado: (2010)

A nuclear targeting system in Plasmodium falciparum
por: Wittayacom, Kanjana, et al.
Publicado: (2010)

Automated counting for Plasmodium falciparum cytoadherence experiments
por: Paton, Douglas, et al.
Publicado: (2011)

Comparison of three molecular methods for the detection and speciation of Plasmodium vivax and Plasmodium falciparum
por: Boonma, Prapaporn, et al.
Publicado: (2007)

Cannot write session to /tmp/vufind_sessions/sess_b43uu9qj8h9dl0jtmlnggau7n1