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Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study
BACKGROUND: Nano-hydroxyapatite (nHA) is a potential ideal biomaterial for bone regeneration. However, studies have yet to characterize the behavior of human osteoblasts derived from alveolar bone on nHA. Thus, the aim of the present study was to evaluate the influence of nHA on the adhesion, prolif...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3994545/ https://www.ncbi.nlm.nih.gov/pubmed/24650194 http://dx.doi.org/10.1186/1472-6831-14-22 |
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author | Pilloni, Andrea Pompa, Giorgio Saccucci, Matteo Di Carlo, Gabriele Rimondini, Lia Brama, Marina Zeza, Blerina Wannenes, Francesca Migliaccio, Silvia |
author_facet | Pilloni, Andrea Pompa, Giorgio Saccucci, Matteo Di Carlo, Gabriele Rimondini, Lia Brama, Marina Zeza, Blerina Wannenes, Francesca Migliaccio, Silvia |
author_sort | Pilloni, Andrea |
collection | PubMed |
description | BACKGROUND: Nano-hydroxyapatite (nHA) is a potential ideal biomaterial for bone regeneration. However, studies have yet to characterize the behavior of human osteoblasts derived from alveolar bone on nHA. Thus, the aim of the present study was to evaluate the influence of nHA on the adhesion, proliferation and differentiation of these alveolar bone-derived cells. METHODS: Primary human alveolar osteoblasts were collected from the alveolar ridge of a male periodontal patient during osseous resective surgery and grown on culture plates coated with either polylysine or polylysine with nano-hydroxyapatite (POL/nHA) composite. The cells were grown and observed for 14 days, and then assessed for potential modifications to osteoblasts homeostasis as evaluated by quantitative reverse transcriptase-polymerase chain reaction (real time RT-PCR), scanning electron microscopy and atomic force microscopy. RESULTS: Real time PCR revealed a significant increase in the expression of the selected markers of osteoblast differentiation (bone morphogenetic protein (BMP)-2,-5,-7, ALP, COLL-1A2, OC, ON) in cells grown on the POL/nHA substrate. In addition, as compared with the POL surface, cells grown on the POL/nHA substrate demonstrated better osteoconductive properties, as demonstrated by the increase in adhesion and spreading, likely as a result of the increased surface roughness of the composite. CONCLUSIONS: The increased expression of BMPs and osteoinductive biomarkers suggest that nano-hydroxyapatite may stimulate the proliferation and differentiation of local alveolar osteoblasts and thus encourage bone regeneration at sites of alveolar bone regeneration. |
format | Online Article Text |
id | pubmed-3994545 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-39945452014-04-23 Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study Pilloni, Andrea Pompa, Giorgio Saccucci, Matteo Di Carlo, Gabriele Rimondini, Lia Brama, Marina Zeza, Blerina Wannenes, Francesca Migliaccio, Silvia BMC Oral Health Research Article BACKGROUND: Nano-hydroxyapatite (nHA) is a potential ideal biomaterial for bone regeneration. However, studies have yet to characterize the behavior of human osteoblasts derived from alveolar bone on nHA. Thus, the aim of the present study was to evaluate the influence of nHA on the adhesion, proliferation and differentiation of these alveolar bone-derived cells. METHODS: Primary human alveolar osteoblasts were collected from the alveolar ridge of a male periodontal patient during osseous resective surgery and grown on culture plates coated with either polylysine or polylysine with nano-hydroxyapatite (POL/nHA) composite. The cells were grown and observed for 14 days, and then assessed for potential modifications to osteoblasts homeostasis as evaluated by quantitative reverse transcriptase-polymerase chain reaction (real time RT-PCR), scanning electron microscopy and atomic force microscopy. RESULTS: Real time PCR revealed a significant increase in the expression of the selected markers of osteoblast differentiation (bone morphogenetic protein (BMP)-2,-5,-7, ALP, COLL-1A2, OC, ON) in cells grown on the POL/nHA substrate. In addition, as compared with the POL surface, cells grown on the POL/nHA substrate demonstrated better osteoconductive properties, as demonstrated by the increase in adhesion and spreading, likely as a result of the increased surface roughness of the composite. CONCLUSIONS: The increased expression of BMPs and osteoinductive biomarkers suggest that nano-hydroxyapatite may stimulate the proliferation and differentiation of local alveolar osteoblasts and thus encourage bone regeneration at sites of alveolar bone regeneration. BioMed Central 2014-03-20 /pmc/articles/PMC3994545/ /pubmed/24650194 http://dx.doi.org/10.1186/1472-6831-14-22 Text en Copyright © 2014 Pilloni et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Pilloni, Andrea Pompa, Giorgio Saccucci, Matteo Di Carlo, Gabriele Rimondini, Lia Brama, Marina Zeza, Blerina Wannenes, Francesca Migliaccio, Silvia Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study |
title | Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study |
title_full | Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study |
title_fullStr | Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study |
title_full_unstemmed | Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study |
title_short | Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study |
title_sort | analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3994545/ https://www.ncbi.nlm.nih.gov/pubmed/24650194 http://dx.doi.org/10.1186/1472-6831-14-22 |
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