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Palatal mucosa derived fibroblasts present an adaptive behavior regarding cytokine secretion when grafted onto the gingival margin

BACKGROUND: Considering that grafted gingival tissue might have to be adapted to the receptor area and that fibroblasts have the ability to respond to bacterial stimuli through the release of various cytokines, this study investigated whether fibroblasts from the palatal mucosa behave differently wh...

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Autores principales: Azevedo, Fabíola Pontes, Morandini, Ana Carolina Faria, Sipert, Carla Renata, Dionísio, Thiago José, Santos, Carlos Ferreira, Damante, Carla Andreotti, de Rezende, Maria Lúcia Rubo, Sant’Ana, Adriana Campos Passanezi, Greghi, Sebastião Luiz Aguiar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3994569/
https://www.ncbi.nlm.nih.gov/pubmed/24646168
http://dx.doi.org/10.1186/1472-6831-14-21
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author Azevedo, Fabíola Pontes
Morandini, Ana Carolina Faria
Sipert, Carla Renata
Dionísio, Thiago José
Santos, Carlos Ferreira
Damante, Carla Andreotti
de Rezende, Maria Lúcia Rubo
Sant’Ana, Adriana Campos Passanezi
Greghi, Sebastião Luiz Aguiar
author_facet Azevedo, Fabíola Pontes
Morandini, Ana Carolina Faria
Sipert, Carla Renata
Dionísio, Thiago José
Santos, Carlos Ferreira
Damante, Carla Andreotti
de Rezende, Maria Lúcia Rubo
Sant’Ana, Adriana Campos Passanezi
Greghi, Sebastião Luiz Aguiar
author_sort Azevedo, Fabíola Pontes
collection PubMed
description BACKGROUND: Considering that grafted gingival tissue might have to be adapted to the receptor area and that fibroblasts have the ability to respond to bacterial stimuli through the release of various cytokines, this study investigated whether fibroblasts from the palatal mucosa behave differently when grafted onto the gingival margin regarding cytokine secretion. METHODS: Biopsies from the palatal mucosa were collected at the time of free gingival graft surgery, and after four months re-collection was performed upon surgery for root coverage. Fibroblasts were isolated by the explant technique, cultured and stimulated with Porphyromonas gingivalis (Pg) and Escherichia coli (Ec) LPS for 24 or 48 h for comparative evaluation of the secretion of cytokines and chemokines, such as IL-6, IL-8/CXCL8, MIP-1α/CCL3, TGF-β, VEGF and CXCL16. Unstimulated cells were used as the control group. Cells were tested for viability through MTT assay, and secretion of cytokines and chemokines was evaluated in the cell supernatants by Enzyme-Linked Immunosorbent Assay (ELISA). RESULTS: Fibroblasts from the palatal mucosa maintained the same secretion pattern of IL-6 when grafted onto the gingival margin. On the contrary, fibroblasts from the marginal gingival graft showed increased secretion of IL-8/CXCL8 even in the absence of stimulation. Interestingly, MIP-1α/CCL3 secretion by fibroblasts from the marginal gingival graft was significantly increased after 48 hours of stimulation with Pg LPS and after 24 h with Ec LPS. Only fibroblasts from the marginal gingival graft showed secretion of TGF-β. VEGF and CXCL16 secretion were not detected by both subsets of fibroblasts. CONCLUSION: Fibroblasts from the palatal mucosa seem to be adapted to local conditions of the site microenvironment when grafted onto the gingival marginal area. This evidence supports the effective participation of fibroblasts in the homeostasis of the marginal periodontium through secretion modulation of important inflammatory mediators.
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spelling pubmed-39945692014-04-23 Palatal mucosa derived fibroblasts present an adaptive behavior regarding cytokine secretion when grafted onto the gingival margin Azevedo, Fabíola Pontes Morandini, Ana Carolina Faria Sipert, Carla Renata Dionísio, Thiago José Santos, Carlos Ferreira Damante, Carla Andreotti de Rezende, Maria Lúcia Rubo Sant’Ana, Adriana Campos Passanezi Greghi, Sebastião Luiz Aguiar BMC Oral Health Research Article BACKGROUND: Considering that grafted gingival tissue might have to be adapted to the receptor area and that fibroblasts have the ability to respond to bacterial stimuli through the release of various cytokines, this study investigated whether fibroblasts from the palatal mucosa behave differently when grafted onto the gingival margin regarding cytokine secretion. METHODS: Biopsies from the palatal mucosa were collected at the time of free gingival graft surgery, and after four months re-collection was performed upon surgery for root coverage. Fibroblasts were isolated by the explant technique, cultured and stimulated with Porphyromonas gingivalis (Pg) and Escherichia coli (Ec) LPS for 24 or 48 h for comparative evaluation of the secretion of cytokines and chemokines, such as IL-6, IL-8/CXCL8, MIP-1α/CCL3, TGF-β, VEGF and CXCL16. Unstimulated cells were used as the control group. Cells were tested for viability through MTT assay, and secretion of cytokines and chemokines was evaluated in the cell supernatants by Enzyme-Linked Immunosorbent Assay (ELISA). RESULTS: Fibroblasts from the palatal mucosa maintained the same secretion pattern of IL-6 when grafted onto the gingival margin. On the contrary, fibroblasts from the marginal gingival graft showed increased secretion of IL-8/CXCL8 even in the absence of stimulation. Interestingly, MIP-1α/CCL3 secretion by fibroblasts from the marginal gingival graft was significantly increased after 48 hours of stimulation with Pg LPS and after 24 h with Ec LPS. Only fibroblasts from the marginal gingival graft showed secretion of TGF-β. VEGF and CXCL16 secretion were not detected by both subsets of fibroblasts. CONCLUSION: Fibroblasts from the palatal mucosa seem to be adapted to local conditions of the site microenvironment when grafted onto the gingival marginal area. This evidence supports the effective participation of fibroblasts in the homeostasis of the marginal periodontium through secretion modulation of important inflammatory mediators. BioMed Central 2014-03-20 /pmc/articles/PMC3994569/ /pubmed/24646168 http://dx.doi.org/10.1186/1472-6831-14-21 Text en Copyright © 2014 Azevedo et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Azevedo, Fabíola Pontes
Morandini, Ana Carolina Faria
Sipert, Carla Renata
Dionísio, Thiago José
Santos, Carlos Ferreira
Damante, Carla Andreotti
de Rezende, Maria Lúcia Rubo
Sant’Ana, Adriana Campos Passanezi
Greghi, Sebastião Luiz Aguiar
Palatal mucosa derived fibroblasts present an adaptive behavior regarding cytokine secretion when grafted onto the gingival margin
title Palatal mucosa derived fibroblasts present an adaptive behavior regarding cytokine secretion when grafted onto the gingival margin
title_full Palatal mucosa derived fibroblasts present an adaptive behavior regarding cytokine secretion when grafted onto the gingival margin
title_fullStr Palatal mucosa derived fibroblasts present an adaptive behavior regarding cytokine secretion when grafted onto the gingival margin
title_full_unstemmed Palatal mucosa derived fibroblasts present an adaptive behavior regarding cytokine secretion when grafted onto the gingival margin
title_short Palatal mucosa derived fibroblasts present an adaptive behavior regarding cytokine secretion when grafted onto the gingival margin
title_sort palatal mucosa derived fibroblasts present an adaptive behavior regarding cytokine secretion when grafted onto the gingival margin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3994569/
https://www.ncbi.nlm.nih.gov/pubmed/24646168
http://dx.doi.org/10.1186/1472-6831-14-21
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