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Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining

The bacterial community and genes involved in geobiocycling of arsenic (As) from sediment impacted by long-term gold mining were characterized through culture-based analysis of As-transforming bacteria and metagenomic studies of the arsC, arrA, and aioA genes. Sediment was collected from the histori...

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Autores principales: Costa, Patrícia S., Scholte, Larissa L. S., Reis, Mariana P., Chaves, Anderson V., Oliveira, Pollyanna L., Itabayana, Luiza B., Suhadolnik, Maria Luiza S., Barbosa, Francisco A. R., Chartone-Souza, Edmar, Nascimento, Andréa M. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995719/
https://www.ncbi.nlm.nih.gov/pubmed/24755825
http://dx.doi.org/10.1371/journal.pone.0095655
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author Costa, Patrícia S.
Scholte, Larissa L. S.
Reis, Mariana P.
Chaves, Anderson V.
Oliveira, Pollyanna L.
Itabayana, Luiza B.
Suhadolnik, Maria Luiza S.
Barbosa, Francisco A. R.
Chartone-Souza, Edmar
Nascimento, Andréa M. A.
author_facet Costa, Patrícia S.
Scholte, Larissa L. S.
Reis, Mariana P.
Chaves, Anderson V.
Oliveira, Pollyanna L.
Itabayana, Luiza B.
Suhadolnik, Maria Luiza S.
Barbosa, Francisco A. R.
Chartone-Souza, Edmar
Nascimento, Andréa M. A.
author_sort Costa, Patrícia S.
collection PubMed
description The bacterial community and genes involved in geobiocycling of arsenic (As) from sediment impacted by long-term gold mining were characterized through culture-based analysis of As-transforming bacteria and metagenomic studies of the arsC, arrA, and aioA genes. Sediment was collected from the historically gold mining impacted Mina stream, located in one of the world’s largest mining regions known as the “Iron Quadrangle”. A total of 123 As-resistant bacteria were recovered from the enrichment cultures, which were phenotypically and genotypically characterized for As-transformation. A diverse As-resistant bacteria community was found through phylogenetic analyses of the 16S rRNA gene. Bacterial isolates were affiliated with Proteobacteria, Firmicutes, and Actinobacteria and were represented by 20 genera. Most were AsV-reducing (72%), whereas AsIII-oxidizing accounted for 20%. Bacteria harboring the arsC gene predominated (85%), followed by aioA (20%) and arrA (7%). Additionally, we identified two novel As-transforming genera, Thermomonas and Pannonibacter. Metagenomic analysis of arsC, aioA, and arrA sequences confirmed the presence of these genes, with arrA sequences being more closely related to uncultured organisms. Evolutionary analyses revealed high genetic similarity between some arsC and aioA sequences obtained from isolates and clone libraries, suggesting that those isolates may represent environmentally important bacteria acting in As speciation. In addition, our findings show that the diversity of arrA genes is wider than earlier described, once none arrA-OTUs were affiliated with known reference strains. Therefore, the molecular diversity of arrA genes is far from being fully explored deserving further attention.
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spelling pubmed-39957192014-04-25 Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining Costa, Patrícia S. Scholte, Larissa L. S. Reis, Mariana P. Chaves, Anderson V. Oliveira, Pollyanna L. Itabayana, Luiza B. Suhadolnik, Maria Luiza S. Barbosa, Francisco A. R. Chartone-Souza, Edmar Nascimento, Andréa M. A. PLoS One Research Article The bacterial community and genes involved in geobiocycling of arsenic (As) from sediment impacted by long-term gold mining were characterized through culture-based analysis of As-transforming bacteria and metagenomic studies of the arsC, arrA, and aioA genes. Sediment was collected from the historically gold mining impacted Mina stream, located in one of the world’s largest mining regions known as the “Iron Quadrangle”. A total of 123 As-resistant bacteria were recovered from the enrichment cultures, which were phenotypically and genotypically characterized for As-transformation. A diverse As-resistant bacteria community was found through phylogenetic analyses of the 16S rRNA gene. Bacterial isolates were affiliated with Proteobacteria, Firmicutes, and Actinobacteria and were represented by 20 genera. Most were AsV-reducing (72%), whereas AsIII-oxidizing accounted for 20%. Bacteria harboring the arsC gene predominated (85%), followed by aioA (20%) and arrA (7%). Additionally, we identified two novel As-transforming genera, Thermomonas and Pannonibacter. Metagenomic analysis of arsC, aioA, and arrA sequences confirmed the presence of these genes, with arrA sequences being more closely related to uncultured organisms. Evolutionary analyses revealed high genetic similarity between some arsC and aioA sequences obtained from isolates and clone libraries, suggesting that those isolates may represent environmentally important bacteria acting in As speciation. In addition, our findings show that the diversity of arrA genes is wider than earlier described, once none arrA-OTUs were affiliated with known reference strains. Therefore, the molecular diversity of arrA genes is far from being fully explored deserving further attention. Public Library of Science 2014-04-22 /pmc/articles/PMC3995719/ /pubmed/24755825 http://dx.doi.org/10.1371/journal.pone.0095655 Text en © 2014 Costa et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Costa, Patrícia S.
Scholte, Larissa L. S.
Reis, Mariana P.
Chaves, Anderson V.
Oliveira, Pollyanna L.
Itabayana, Luiza B.
Suhadolnik, Maria Luiza S.
Barbosa, Francisco A. R.
Chartone-Souza, Edmar
Nascimento, Andréa M. A.
Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining
title Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining
title_full Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining
title_fullStr Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining
title_full_unstemmed Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining
title_short Bacteria and Genes Involved in Arsenic Speciation in Sediment Impacted by Long-Term Gold Mining
title_sort bacteria and genes involved in arsenic speciation in sediment impacted by long-term gold mining
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995719/
https://www.ncbi.nlm.nih.gov/pubmed/24755825
http://dx.doi.org/10.1371/journal.pone.0095655
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