Cargando…

DNA Sequence Heterogeneity of Campylobacter jejuni CJIE4 Prophages and Expression of Prophage Genes

Campylobacter jejuni carry temperate bacteriophages that can affect the biology or virulence of the host bacterium. Known effects include genomic rearrangements and resistance to DNA transformation. C. jejuni prophage CJIE1 shows sequence variability and variability in the content of morons. Homolog...

Descripción completa

Detalles Bibliográficos
Autores principales: Clark, Clifford G., Chong, Patrick M., McCorrister, Stuart J., Mabon, Philip, Walker, Matthew, Westmacott, Garrett R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995785/
https://www.ncbi.nlm.nih.gov/pubmed/24756024
http://dx.doi.org/10.1371/journal.pone.0095349
_version_ 1782312936243462144
author Clark, Clifford G.
Chong, Patrick M.
McCorrister, Stuart J.
Mabon, Philip
Walker, Matthew
Westmacott, Garrett R.
author_facet Clark, Clifford G.
Chong, Patrick M.
McCorrister, Stuart J.
Mabon, Philip
Walker, Matthew
Westmacott, Garrett R.
author_sort Clark, Clifford G.
collection PubMed
description Campylobacter jejuni carry temperate bacteriophages that can affect the biology or virulence of the host bacterium. Known effects include genomic rearrangements and resistance to DNA transformation. C. jejuni prophage CJIE1 shows sequence variability and variability in the content of morons. Homologs of the CJIE1 prophage enhance both adherence and invasion to cells in culture and increase the expression of a specific subset of bacterial genes. Other C. jejuni temperate phages have so far not been well characterized. In this study we describe investigations into the DNA sequence variability and protein expression in a second prophage, CJIE4. CJIE4 sequences were obtained de novo from DNA sequencing of five C. jejuni isolates, as well as from whole genome sequences submitted to GenBank by other research groups. These CJIE4 DNA sequences were heterogenous, with several different insertions/deletions (indels) in different parts of the prophage genome. Two variants of a 3–4 kb region inserted within CJIE4 had different gene content that distinguished two major conserved CJIE4 prophage families. Additional indels were detected throughout the prophage. Detection of proteins in the five isolates characterized in our laboratory in isobaric Tags for Relative and Absolute Quantitation (iTRAQ) experiments indicated that prophage proteins within each of the two large indel variants were expressed during growth of the bacteria on Mueller Hinton agar plates. These proteins included the extracellular DNase associated with resistance to DNA transformation and prophage repressor proteins. Other proteins associated with known or suspected roles in prophage biology were also expressed from CJIE4, including capsid protein, the phage integrase, and MazF, a type II toxin-antitoxin system protein. Together with the results previously obtained for the CJIE1 prophage these results demonstrate that sequence variability and expression of moron genes are both general properties of temperate bacteriophages in C. jejuni.
format Online
Article
Text
id pubmed-3995785
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-39957852014-04-25 DNA Sequence Heterogeneity of Campylobacter jejuni CJIE4 Prophages and Expression of Prophage Genes Clark, Clifford G. Chong, Patrick M. McCorrister, Stuart J. Mabon, Philip Walker, Matthew Westmacott, Garrett R. PLoS One Research Article Campylobacter jejuni carry temperate bacteriophages that can affect the biology or virulence of the host bacterium. Known effects include genomic rearrangements and resistance to DNA transformation. C. jejuni prophage CJIE1 shows sequence variability and variability in the content of morons. Homologs of the CJIE1 prophage enhance both adherence and invasion to cells in culture and increase the expression of a specific subset of bacterial genes. Other C. jejuni temperate phages have so far not been well characterized. In this study we describe investigations into the DNA sequence variability and protein expression in a second prophage, CJIE4. CJIE4 sequences were obtained de novo from DNA sequencing of five C. jejuni isolates, as well as from whole genome sequences submitted to GenBank by other research groups. These CJIE4 DNA sequences were heterogenous, with several different insertions/deletions (indels) in different parts of the prophage genome. Two variants of a 3–4 kb region inserted within CJIE4 had different gene content that distinguished two major conserved CJIE4 prophage families. Additional indels were detected throughout the prophage. Detection of proteins in the five isolates characterized in our laboratory in isobaric Tags for Relative and Absolute Quantitation (iTRAQ) experiments indicated that prophage proteins within each of the two large indel variants were expressed during growth of the bacteria on Mueller Hinton agar plates. These proteins included the extracellular DNase associated with resistance to DNA transformation and prophage repressor proteins. Other proteins associated with known or suspected roles in prophage biology were also expressed from CJIE4, including capsid protein, the phage integrase, and MazF, a type II toxin-antitoxin system protein. Together with the results previously obtained for the CJIE1 prophage these results demonstrate that sequence variability and expression of moron genes are both general properties of temperate bacteriophages in C. jejuni. Public Library of Science 2014-04-22 /pmc/articles/PMC3995785/ /pubmed/24756024 http://dx.doi.org/10.1371/journal.pone.0095349 Text en © 2014 Clark et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Clark, Clifford G.
Chong, Patrick M.
McCorrister, Stuart J.
Mabon, Philip
Walker, Matthew
Westmacott, Garrett R.
DNA Sequence Heterogeneity of Campylobacter jejuni CJIE4 Prophages and Expression of Prophage Genes
title DNA Sequence Heterogeneity of Campylobacter jejuni CJIE4 Prophages and Expression of Prophage Genes
title_full DNA Sequence Heterogeneity of Campylobacter jejuni CJIE4 Prophages and Expression of Prophage Genes
title_fullStr DNA Sequence Heterogeneity of Campylobacter jejuni CJIE4 Prophages and Expression of Prophage Genes
title_full_unstemmed DNA Sequence Heterogeneity of Campylobacter jejuni CJIE4 Prophages and Expression of Prophage Genes
title_short DNA Sequence Heterogeneity of Campylobacter jejuni CJIE4 Prophages and Expression of Prophage Genes
title_sort dna sequence heterogeneity of campylobacter jejuni cjie4 prophages and expression of prophage genes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3995785/
https://www.ncbi.nlm.nih.gov/pubmed/24756024
http://dx.doi.org/10.1371/journal.pone.0095349
work_keys_str_mv AT clarkcliffordg dnasequenceheterogeneityofcampylobacterjejunicjie4prophagesandexpressionofprophagegenes
AT chongpatrickm dnasequenceheterogeneityofcampylobacterjejunicjie4prophagesandexpressionofprophagegenes
AT mccorristerstuartj dnasequenceheterogeneityofcampylobacterjejunicjie4prophagesandexpressionofprophagegenes
AT mabonphilip dnasequenceheterogeneityofcampylobacterjejunicjie4prophagesandexpressionofprophagegenes
AT walkermatthew dnasequenceheterogeneityofcampylobacterjejunicjie4prophagesandexpressionofprophagegenes
AT westmacottgarrettr dnasequenceheterogeneityofcampylobacterjejunicjie4prophagesandexpressionofprophagegenes