Reductive Methylation and Mutation of an Anthrax Toxin Fusion Protein Modulates its Stability and Cytotoxicity

We characterized an anti-cancer fusion protein consisting of anthrax lethal factor (LF) and the catalytic domain of Pseudomonas exotoxin A by (i) mutating the N-terminal amino acids and by (ii) reductive methylation to dimethylate all lysines. Dimethylation of lysines was achieved quantitatively and...

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Detalles Bibliográficos
Autores principales: Bachran, Christopher, Gupta, Pradeep K., Bachran, Silke, Leysath, Clinton E., Hoover, Benjamin, Fattah, Rasem J., Leppla, Stephen H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3996465/
https://www.ncbi.nlm.nih.gov/pubmed/24755540
http://dx.doi.org/10.1038/srep04754

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