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Bacteriophage T7 DNA polymerase – sequenase

An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: (1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; (2) high processivity; (3) high fidelity in the absence of proofreading/exonuc...

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Detalles Bibliográficos
Autor principal: Zhu, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997047/
https://www.ncbi.nlm.nih.gov/pubmed/24795710
http://dx.doi.org/10.3389/fmicb.2014.00181
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author Zhu, Bin
author_facet Zhu, Bin
author_sort Zhu, Bin
collection PubMed
description An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: (1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; (2) high processivity; (3) high fidelity in the absence of proofreading/exonuclease activity; and (4) production of clear and uniform signals for detection. The DNA polymerase encoded by bacteriophage T7 is naturally endowed with or can be engineered to have all these characteristics. The chemically or genetically modified enzyme (Sequenase) expedited significantly the development of DNA sequencing technology. This article reviews the history of studies on T7 DNA polymerase with emphasis on the serial key steps leading to its use in DNA sequencing. Lessons from the study and development of T7 DNA polymerase have and will continue to enlighten the characterization of novel DNA polymerases from newly discovered microbes and their modification for use in biotechnology.
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spelling pubmed-39970472014-05-02 Bacteriophage T7 DNA polymerase – sequenase Zhu, Bin Front Microbiol Microbiology An ideal DNA polymerase for chain-terminating DNA sequencing should possess the following features: (1) incorporate dideoxy- and other modified nucleotides at an efficiency similar to that of the cognate deoxynucleotides; (2) high processivity; (3) high fidelity in the absence of proofreading/exonuclease activity; and (4) production of clear and uniform signals for detection. The DNA polymerase encoded by bacteriophage T7 is naturally endowed with or can be engineered to have all these characteristics. The chemically or genetically modified enzyme (Sequenase) expedited significantly the development of DNA sequencing technology. This article reviews the history of studies on T7 DNA polymerase with emphasis on the serial key steps leading to its use in DNA sequencing. Lessons from the study and development of T7 DNA polymerase have and will continue to enlighten the characterization of novel DNA polymerases from newly discovered microbes and their modification for use in biotechnology. Frontiers Media S.A. 2014-04-16 /pmc/articles/PMC3997047/ /pubmed/24795710 http://dx.doi.org/10.3389/fmicb.2014.00181 Text en Copyright © 2014 Zhu. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhu, Bin
Bacteriophage T7 DNA polymerase – sequenase
title Bacteriophage T7 DNA polymerase – sequenase
title_full Bacteriophage T7 DNA polymerase – sequenase
title_fullStr Bacteriophage T7 DNA polymerase – sequenase
title_full_unstemmed Bacteriophage T7 DNA polymerase – sequenase
title_short Bacteriophage T7 DNA polymerase – sequenase
title_sort bacteriophage t7 dna polymerase – sequenase
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997047/
https://www.ncbi.nlm.nih.gov/pubmed/24795710
http://dx.doi.org/10.3389/fmicb.2014.00181
work_keys_str_mv AT zhubin bacteriophaget7dnapolymerasesequenase