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Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold
Cells, growth factors, and scaffolds are the three main factors required to create a tissue-engineered construct. After the appearance of bovine spongiform encephalopathy (BSE), considerable attention has therefore been focused on nonbovine materials. In this study, we examined the properties of a c...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997083/ https://www.ncbi.nlm.nih.gov/pubmed/24804246 http://dx.doi.org/10.1155/2014/786892 |
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author | Ikeda, Takeshi Ikeda, Kahori Yamamoto, Kouhei Ishizaki, Hidetaka Yoshizawa, Yuu Yanagiguchi, Kajiro Yamada, Shizuka Hayashi, Yoshihiko |
author_facet | Ikeda, Takeshi Ikeda, Kahori Yamamoto, Kouhei Ishizaki, Hidetaka Yoshizawa, Yuu Yanagiguchi, Kajiro Yamada, Shizuka Hayashi, Yoshihiko |
author_sort | Ikeda, Takeshi |
collection | PubMed |
description | Cells, growth factors, and scaffolds are the three main factors required to create a tissue-engineered construct. After the appearance of bovine spongiform encephalopathy (BSE), considerable attention has therefore been focused on nonbovine materials. In this study, we examined the properties of a chitosan porous scaffold. A porous chitosan sponge was prepared by the controlled freezing and lyophilization of different concentrations of chitosan solutions. The materials were examined by scanning electron microscopy, and the porosity, tensile strength, and basic fibroblast growth factor (bFGF) release profiles from chitosan sponge were examined in vitro. The morphology of the chitosan scaffolds presented a typical microporous structure, with the pore size ranging from 50 to 200 μm. The porosity of chitosan scaffolds with different concentrations was approximately 75–85%. A decreasing tendency for porosity was observed as the concentration of the chitosan increased. The relationship between the tensile properties and chitosan concentration indicated that the ultimate tensile strength for the sponge increased with a higher concentration. The in vitro bFGF release study showed that the higher the concentration of chitosan solution became, the longer the releasing time of the bFGF from the chitosan sponge was. |
format | Online Article Text |
id | pubmed-3997083 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-39970832014-05-06 Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold Ikeda, Takeshi Ikeda, Kahori Yamamoto, Kouhei Ishizaki, Hidetaka Yoshizawa, Yuu Yanagiguchi, Kajiro Yamada, Shizuka Hayashi, Yoshihiko Biomed Res Int Research Article Cells, growth factors, and scaffolds are the three main factors required to create a tissue-engineered construct. After the appearance of bovine spongiform encephalopathy (BSE), considerable attention has therefore been focused on nonbovine materials. In this study, we examined the properties of a chitosan porous scaffold. A porous chitosan sponge was prepared by the controlled freezing and lyophilization of different concentrations of chitosan solutions. The materials were examined by scanning electron microscopy, and the porosity, tensile strength, and basic fibroblast growth factor (bFGF) release profiles from chitosan sponge were examined in vitro. The morphology of the chitosan scaffolds presented a typical microporous structure, with the pore size ranging from 50 to 200 μm. The porosity of chitosan scaffolds with different concentrations was approximately 75–85%. A decreasing tendency for porosity was observed as the concentration of the chitosan increased. The relationship between the tensile properties and chitosan concentration indicated that the ultimate tensile strength for the sponge increased with a higher concentration. The in vitro bFGF release study showed that the higher the concentration of chitosan solution became, the longer the releasing time of the bFGF from the chitosan sponge was. Hindawi Publishing Corporation 2014 2014-04-06 /pmc/articles/PMC3997083/ /pubmed/24804246 http://dx.doi.org/10.1155/2014/786892 Text en Copyright © 2014 Takeshi Ikeda et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ikeda, Takeshi Ikeda, Kahori Yamamoto, Kouhei Ishizaki, Hidetaka Yoshizawa, Yuu Yanagiguchi, Kajiro Yamada, Shizuka Hayashi, Yoshihiko Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold |
title | Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold |
title_full | Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold |
title_fullStr | Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold |
title_full_unstemmed | Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold |
title_short | Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold |
title_sort | fabrication and characteristics of chitosan sponge as a tissue engineering scaffold |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997083/ https://www.ncbi.nlm.nih.gov/pubmed/24804246 http://dx.doi.org/10.1155/2014/786892 |
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