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Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold

Cells, growth factors, and scaffolds are the three main factors required to create a tissue-engineered construct. After the appearance of bovine spongiform encephalopathy (BSE), considerable attention has therefore been focused on nonbovine materials. In this study, we examined the properties of a c...

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Autores principales: Ikeda, Takeshi, Ikeda, Kahori, Yamamoto, Kouhei, Ishizaki, Hidetaka, Yoshizawa, Yuu, Yanagiguchi, Kajiro, Yamada, Shizuka, Hayashi, Yoshihiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997083/
https://www.ncbi.nlm.nih.gov/pubmed/24804246
http://dx.doi.org/10.1155/2014/786892
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author Ikeda, Takeshi
Ikeda, Kahori
Yamamoto, Kouhei
Ishizaki, Hidetaka
Yoshizawa, Yuu
Yanagiguchi, Kajiro
Yamada, Shizuka
Hayashi, Yoshihiko
author_facet Ikeda, Takeshi
Ikeda, Kahori
Yamamoto, Kouhei
Ishizaki, Hidetaka
Yoshizawa, Yuu
Yanagiguchi, Kajiro
Yamada, Shizuka
Hayashi, Yoshihiko
author_sort Ikeda, Takeshi
collection PubMed
description Cells, growth factors, and scaffolds are the three main factors required to create a tissue-engineered construct. After the appearance of bovine spongiform encephalopathy (BSE), considerable attention has therefore been focused on nonbovine materials. In this study, we examined the properties of a chitosan porous scaffold. A porous chitosan sponge was prepared by the controlled freezing and lyophilization of different concentrations of chitosan solutions. The materials were examined by scanning electron microscopy, and the porosity, tensile strength, and basic fibroblast growth factor (bFGF) release profiles from chitosan sponge were examined in vitro. The morphology of the chitosan scaffolds presented a typical microporous structure, with the pore size ranging from 50 to 200 μm. The porosity of chitosan scaffolds with different concentrations was approximately 75–85%. A decreasing tendency for porosity was observed as the concentration of the chitosan increased. The relationship between the tensile properties and chitosan concentration indicated that the ultimate tensile strength for the sponge increased with a higher concentration. The in vitro bFGF release study showed that the higher the concentration of chitosan solution became, the longer the releasing time of the bFGF from the chitosan sponge was.
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spelling pubmed-39970832014-05-06 Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold Ikeda, Takeshi Ikeda, Kahori Yamamoto, Kouhei Ishizaki, Hidetaka Yoshizawa, Yuu Yanagiguchi, Kajiro Yamada, Shizuka Hayashi, Yoshihiko Biomed Res Int Research Article Cells, growth factors, and scaffolds are the three main factors required to create a tissue-engineered construct. After the appearance of bovine spongiform encephalopathy (BSE), considerable attention has therefore been focused on nonbovine materials. In this study, we examined the properties of a chitosan porous scaffold. A porous chitosan sponge was prepared by the controlled freezing and lyophilization of different concentrations of chitosan solutions. The materials were examined by scanning electron microscopy, and the porosity, tensile strength, and basic fibroblast growth factor (bFGF) release profiles from chitosan sponge were examined in vitro. The morphology of the chitosan scaffolds presented a typical microporous structure, with the pore size ranging from 50 to 200 μm. The porosity of chitosan scaffolds with different concentrations was approximately 75–85%. A decreasing tendency for porosity was observed as the concentration of the chitosan increased. The relationship between the tensile properties and chitosan concentration indicated that the ultimate tensile strength for the sponge increased with a higher concentration. The in vitro bFGF release study showed that the higher the concentration of chitosan solution became, the longer the releasing time of the bFGF from the chitosan sponge was. Hindawi Publishing Corporation 2014 2014-04-06 /pmc/articles/PMC3997083/ /pubmed/24804246 http://dx.doi.org/10.1155/2014/786892 Text en Copyright © 2014 Takeshi Ikeda et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ikeda, Takeshi
Ikeda, Kahori
Yamamoto, Kouhei
Ishizaki, Hidetaka
Yoshizawa, Yuu
Yanagiguchi, Kajiro
Yamada, Shizuka
Hayashi, Yoshihiko
Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold
title Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold
title_full Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold
title_fullStr Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold
title_full_unstemmed Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold
title_short Fabrication and Characteristics of Chitosan Sponge as a Tissue Engineering Scaffold
title_sort fabrication and characteristics of chitosan sponge as a tissue engineering scaffold
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997083/
https://www.ncbi.nlm.nih.gov/pubmed/24804246
http://dx.doi.org/10.1155/2014/786892
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