Cargando…

The Morphology and Functions of Articular Chondrocytes on a Honeycomb-Patterned Surface

The present study investigated the potential of a novel micropatterned substrate for neocartilage formation. Articular chondrocytes were cultured on poly(ɛ-caprolactone) materials whose surfaces were either flat or honeycomb-patterned. The latter was prepared using a novel self-organization techniqu...

Descripción completa

Detalles Bibliográficos
Autores principales: Eniwumide, Joshua O., Tanaka, Masaru, Nagai, Nobuhiro, Morita, Yuka, de Bruijn, Joost, Yamamoto, Sadaaki, Onodera, Shin, Kondo, Eiji, Yasuda, Kazunori, Shimomura, Masatsugu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997153/
https://www.ncbi.nlm.nih.gov/pubmed/24804237
http://dx.doi.org/10.1155/2014/710354
_version_ 1782313149533257728
author Eniwumide, Joshua O.
Tanaka, Masaru
Nagai, Nobuhiro
Morita, Yuka
de Bruijn, Joost
Yamamoto, Sadaaki
Onodera, Shin
Kondo, Eiji
Yasuda, Kazunori
Shimomura, Masatsugu
author_facet Eniwumide, Joshua O.
Tanaka, Masaru
Nagai, Nobuhiro
Morita, Yuka
de Bruijn, Joost
Yamamoto, Sadaaki
Onodera, Shin
Kondo, Eiji
Yasuda, Kazunori
Shimomura, Masatsugu
author_sort Eniwumide, Joshua O.
collection PubMed
description The present study investigated the potential of a novel micropatterned substrate for neocartilage formation. Articular chondrocytes were cultured on poly(ɛ-caprolactone) materials whose surfaces were either flat or honeycomb-patterned. The latter was prepared using a novel self-organization technique, while the former, was prepared by spin-coating. The chondrocytes attached and proliferated on both surfaces. On the honeycomb films, chondrocytes were found at the top surface and encased within the 10 μm pores. Meanwhile, chondrocytes on the spin-coated surface flattened out. Accumulation of DNA and keratin sulphate was comparatively higher on the honeycomb films within the first 7 days. At their respective peaks, DNA concentration increased on the honeycomb and flat surfaces by approximately 210% and 400% of their day 1 values, respectively. However, cultures on the flat surface took longer to peak. Extracellular Matrix (ECM) concentrations peaked at 900% and 320% increases for the honeycomb and flat cultures. Type II collagen was upregulated on the honeycomb and flat surfaces by as much as 28% and 25% of their day 1 values, while aggrecan was downregulated with time, by 3.4% and 7.4%. These initial results demonstrate the potential usefulness of honeycomb-based scaffolds during early cultures neocartilage and soft tissue engineering.
format Online
Article
Text
id pubmed-3997153
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-39971532014-05-06 The Morphology and Functions of Articular Chondrocytes on a Honeycomb-Patterned Surface Eniwumide, Joshua O. Tanaka, Masaru Nagai, Nobuhiro Morita, Yuka de Bruijn, Joost Yamamoto, Sadaaki Onodera, Shin Kondo, Eiji Yasuda, Kazunori Shimomura, Masatsugu Biomed Res Int Research Article The present study investigated the potential of a novel micropatterned substrate for neocartilage formation. Articular chondrocytes were cultured on poly(ɛ-caprolactone) materials whose surfaces were either flat or honeycomb-patterned. The latter was prepared using a novel self-organization technique, while the former, was prepared by spin-coating. The chondrocytes attached and proliferated on both surfaces. On the honeycomb films, chondrocytes were found at the top surface and encased within the 10 μm pores. Meanwhile, chondrocytes on the spin-coated surface flattened out. Accumulation of DNA and keratin sulphate was comparatively higher on the honeycomb films within the first 7 days. At their respective peaks, DNA concentration increased on the honeycomb and flat surfaces by approximately 210% and 400% of their day 1 values, respectively. However, cultures on the flat surface took longer to peak. Extracellular Matrix (ECM) concentrations peaked at 900% and 320% increases for the honeycomb and flat cultures. Type II collagen was upregulated on the honeycomb and flat surfaces by as much as 28% and 25% of their day 1 values, while aggrecan was downregulated with time, by 3.4% and 7.4%. These initial results demonstrate the potential usefulness of honeycomb-based scaffolds during early cultures neocartilage and soft tissue engineering. Hindawi Publishing Corporation 2014 2014-04-06 /pmc/articles/PMC3997153/ /pubmed/24804237 http://dx.doi.org/10.1155/2014/710354 Text en Copyright © 2014 Joshua O. Eniwumide et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Eniwumide, Joshua O.
Tanaka, Masaru
Nagai, Nobuhiro
Morita, Yuka
de Bruijn, Joost
Yamamoto, Sadaaki
Onodera, Shin
Kondo, Eiji
Yasuda, Kazunori
Shimomura, Masatsugu
The Morphology and Functions of Articular Chondrocytes on a Honeycomb-Patterned Surface
title The Morphology and Functions of Articular Chondrocytes on a Honeycomb-Patterned Surface
title_full The Morphology and Functions of Articular Chondrocytes on a Honeycomb-Patterned Surface
title_fullStr The Morphology and Functions of Articular Chondrocytes on a Honeycomb-Patterned Surface
title_full_unstemmed The Morphology and Functions of Articular Chondrocytes on a Honeycomb-Patterned Surface
title_short The Morphology and Functions of Articular Chondrocytes on a Honeycomb-Patterned Surface
title_sort morphology and functions of articular chondrocytes on a honeycomb-patterned surface
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997153/
https://www.ncbi.nlm.nih.gov/pubmed/24804237
http://dx.doi.org/10.1155/2014/710354
work_keys_str_mv AT eniwumidejoshuao themorphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT tanakamasaru themorphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT nagainobuhiro themorphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT moritayuka themorphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT debruijnjoost themorphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT yamamotosadaaki themorphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT onoderashin themorphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT kondoeiji themorphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT yasudakazunori themorphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT shimomuramasatsugu themorphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT eniwumidejoshuao morphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT tanakamasaru morphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT nagainobuhiro morphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT moritayuka morphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT debruijnjoost morphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT yamamotosadaaki morphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT onoderashin morphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT kondoeiji morphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT yasudakazunori morphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface
AT shimomuramasatsugu morphologyandfunctionsofarticularchondrocytesonahoneycombpatternedsurface