Cargando…

CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells

Maintenance, repair and renewal of the epidermis are thought to depend on a pool of dedicated epidermal stem cells. Like for many somatic tissues, isolation of a nearly pure population of stem cells is a primary goal in cutaneous biology. We used a quantitative transplantation assay, using injection...

Descripción completa

Detalles Bibliográficos
Autores principales: Charruyer, A, Strachan, LR, Yue, L, Toth, AS, Mancianti, ML, Ghadially, R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997791/
https://www.ncbi.nlm.nih.gov/pubmed/22763787
http://dx.doi.org/10.1038/jid.2012.196
_version_ 1782313231969157120
author Charruyer, A
Strachan, LR
Yue, L
Toth, AS
Mancianti, ML
Ghadially, R
author_facet Charruyer, A
Strachan, LR
Yue, L
Toth, AS
Mancianti, ML
Ghadially, R
author_sort Charruyer, A
collection PubMed
description Maintenance, repair and renewal of the epidermis are thought to depend on a pool of dedicated epidermal stem cells. Like for many somatic tissues, isolation of a nearly pure population of stem cells is a primary goal in cutaneous biology. We used a quantitative transplantation assay, using injection of keratinocytes into subcutis combined with limiting dilution analysis, to assess the long-term repopulating ability of putative murine epidermal stem populations. Putative epidermal stem cell populations were isolated by FACS sorting. The CD133(+) population and the subpopulation of CD133(+) cells that exhibits high mitochondrial membrane potential (DΨm(hi)), were enriched for long-term repopulating epidermal stem cells vs. unfractionated cells (3.9 and 5.2-fold, respectively). Evidence for self-renewal capacity was obtained by serial transplantation of long-term epidermal repopulating units derived from CD133(+) and CD133(+)ΔΨm(hi) keratinocytes. CD133(+) keratinocytes were multipotent and produced significantly more hair follicles than CD133(−) cells. CD133(+) cells were a subset of the previously described integrin α6(+)CD34(+) bulge cell population and 28.9±8.6% were label retaining cells. Thus, murine keratinocytes within the CD133(+) and CD133(+)ΔΨm(hi) populations contain epidermal stem cells that regenerate epidermis for the long-term, are self-renewing, multipotent, and label-retaining cells.
format Online
Article
Text
id pubmed-3997791
institution National Center for Biotechnology Information
language English
publishDate 2012
record_format MEDLINE/PubMed
spelling pubmed-39977912014-04-24 CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells Charruyer, A Strachan, LR Yue, L Toth, AS Mancianti, ML Ghadially, R J Invest Dermatol Article Maintenance, repair and renewal of the epidermis are thought to depend on a pool of dedicated epidermal stem cells. Like for many somatic tissues, isolation of a nearly pure population of stem cells is a primary goal in cutaneous biology. We used a quantitative transplantation assay, using injection of keratinocytes into subcutis combined with limiting dilution analysis, to assess the long-term repopulating ability of putative murine epidermal stem populations. Putative epidermal stem cell populations were isolated by FACS sorting. The CD133(+) population and the subpopulation of CD133(+) cells that exhibits high mitochondrial membrane potential (DΨm(hi)), were enriched for long-term repopulating epidermal stem cells vs. unfractionated cells (3.9 and 5.2-fold, respectively). Evidence for self-renewal capacity was obtained by serial transplantation of long-term epidermal repopulating units derived from CD133(+) and CD133(+)ΔΨm(hi) keratinocytes. CD133(+) keratinocytes were multipotent and produced significantly more hair follicles than CD133(−) cells. CD133(+) cells were a subset of the previously described integrin α6(+)CD34(+) bulge cell population and 28.9±8.6% were label retaining cells. Thus, murine keratinocytes within the CD133(+) and CD133(+)ΔΨm(hi) populations contain epidermal stem cells that regenerate epidermis for the long-term, are self-renewing, multipotent, and label-retaining cells. 2012-07-05 2012-11 /pmc/articles/PMC3997791/ /pubmed/22763787 http://dx.doi.org/10.1038/jid.2012.196 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Charruyer, A
Strachan, LR
Yue, L
Toth, AS
Mancianti, ML
Ghadially, R
CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells
title CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells
title_full CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells
title_fullStr CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells
title_full_unstemmed CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells
title_short CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells
title_sort cd133 is a marker for long-term repopulating murine epidermal stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997791/
https://www.ncbi.nlm.nih.gov/pubmed/22763787
http://dx.doi.org/10.1038/jid.2012.196
work_keys_str_mv AT charruyera cd133isamarkerforlongtermrepopulatingmurineepidermalstemcells
AT strachanlr cd133isamarkerforlongtermrepopulatingmurineepidermalstemcells
AT yuel cd133isamarkerforlongtermrepopulatingmurineepidermalstemcells
AT tothas cd133isamarkerforlongtermrepopulatingmurineepidermalstemcells
AT manciantiml cd133isamarkerforlongtermrepopulatingmurineepidermalstemcells
AT ghadiallyr cd133isamarkerforlongtermrepopulatingmurineepidermalstemcells