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CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells
Maintenance, repair and renewal of the epidermis are thought to depend on a pool of dedicated epidermal stem cells. Like for many somatic tissues, isolation of a nearly pure population of stem cells is a primary goal in cutaneous biology. We used a quantitative transplantation assay, using injection...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997791/ https://www.ncbi.nlm.nih.gov/pubmed/22763787 http://dx.doi.org/10.1038/jid.2012.196 |
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author | Charruyer, A Strachan, LR Yue, L Toth, AS Mancianti, ML Ghadially, R |
author_facet | Charruyer, A Strachan, LR Yue, L Toth, AS Mancianti, ML Ghadially, R |
author_sort | Charruyer, A |
collection | PubMed |
description | Maintenance, repair and renewal of the epidermis are thought to depend on a pool of dedicated epidermal stem cells. Like for many somatic tissues, isolation of a nearly pure population of stem cells is a primary goal in cutaneous biology. We used a quantitative transplantation assay, using injection of keratinocytes into subcutis combined with limiting dilution analysis, to assess the long-term repopulating ability of putative murine epidermal stem populations. Putative epidermal stem cell populations were isolated by FACS sorting. The CD133(+) population and the subpopulation of CD133(+) cells that exhibits high mitochondrial membrane potential (DΨm(hi)), were enriched for long-term repopulating epidermal stem cells vs. unfractionated cells (3.9 and 5.2-fold, respectively). Evidence for self-renewal capacity was obtained by serial transplantation of long-term epidermal repopulating units derived from CD133(+) and CD133(+)ΔΨm(hi) keratinocytes. CD133(+) keratinocytes were multipotent and produced significantly more hair follicles than CD133(−) cells. CD133(+) cells were a subset of the previously described integrin α6(+)CD34(+) bulge cell population and 28.9±8.6% were label retaining cells. Thus, murine keratinocytes within the CD133(+) and CD133(+)ΔΨm(hi) populations contain epidermal stem cells that regenerate epidermis for the long-term, are self-renewing, multipotent, and label-retaining cells. |
format | Online Article Text |
id | pubmed-3997791 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
record_format | MEDLINE/PubMed |
spelling | pubmed-39977912014-04-24 CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells Charruyer, A Strachan, LR Yue, L Toth, AS Mancianti, ML Ghadially, R J Invest Dermatol Article Maintenance, repair and renewal of the epidermis are thought to depend on a pool of dedicated epidermal stem cells. Like for many somatic tissues, isolation of a nearly pure population of stem cells is a primary goal in cutaneous biology. We used a quantitative transplantation assay, using injection of keratinocytes into subcutis combined with limiting dilution analysis, to assess the long-term repopulating ability of putative murine epidermal stem populations. Putative epidermal stem cell populations were isolated by FACS sorting. The CD133(+) population and the subpopulation of CD133(+) cells that exhibits high mitochondrial membrane potential (DΨm(hi)), were enriched for long-term repopulating epidermal stem cells vs. unfractionated cells (3.9 and 5.2-fold, respectively). Evidence for self-renewal capacity was obtained by serial transplantation of long-term epidermal repopulating units derived from CD133(+) and CD133(+)ΔΨm(hi) keratinocytes. CD133(+) keratinocytes were multipotent and produced significantly more hair follicles than CD133(−) cells. CD133(+) cells were a subset of the previously described integrin α6(+)CD34(+) bulge cell population and 28.9±8.6% were label retaining cells. Thus, murine keratinocytes within the CD133(+) and CD133(+)ΔΨm(hi) populations contain epidermal stem cells that regenerate epidermis for the long-term, are self-renewing, multipotent, and label-retaining cells. 2012-07-05 2012-11 /pmc/articles/PMC3997791/ /pubmed/22763787 http://dx.doi.org/10.1038/jid.2012.196 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Charruyer, A Strachan, LR Yue, L Toth, AS Mancianti, ML Ghadially, R CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells |
title | CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells |
title_full | CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells |
title_fullStr | CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells |
title_full_unstemmed | CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells |
title_short | CD133 Is a Marker For Long-Term Repopulating Murine Epidermal Stem Cells |
title_sort | cd133 is a marker for long-term repopulating murine epidermal stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997791/ https://www.ncbi.nlm.nih.gov/pubmed/22763787 http://dx.doi.org/10.1038/jid.2012.196 |
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