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Reliable reference miRNAs for quantitative gene expression analysis of stress responses in Caenorhabditis elegans

BACKGROUND: Quantitative real-time PCR (qPCR) has become the “gold standard” for measuring expression levels of individual miRNAs. However, little is known about the validity of reference miRNAs, the improper use of which can result in misleading interpretation of data. RESULTS: Here we undertook a...

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Detalles Bibliográficos
Autores principales: Kagias, Konstantinos, Podolska, Agnieszka, Pocock, Roger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3997968/
https://www.ncbi.nlm.nih.gov/pubmed/24656064
http://dx.doi.org/10.1186/1471-2164-15-222