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Quantification of verbascoside in medicinal species of Phlomis and their genetic relationships

BACKGROUND: The genus Phlomis (Lamiaceae) is introduced by its valuable medicinal species, of which 17 species are growing wildly and ten of them are exclusively endemic of Iran. The main phytochemical characteristic of this genus is presence of iridoid glycosides including ipolamide, auroside, lami...

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Autores principales: Sarkhail, Parisa, Nikan, Marjan, Sarkheil, Pantea, Gohari, Ahmad R, Ajani, Yousef, Hosseini, Rohollah, Hadjiakhoondi, Abbass, Saeidnia, Soodabeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998186/
https://www.ncbi.nlm.nih.gov/pubmed/24650578
http://dx.doi.org/10.1186/2008-2231-22-32
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author Sarkhail, Parisa
Nikan, Marjan
Sarkheil, Pantea
Gohari, Ahmad R
Ajani, Yousef
Hosseini, Rohollah
Hadjiakhoondi, Abbass
Saeidnia, Soodabeh
author_facet Sarkhail, Parisa
Nikan, Marjan
Sarkheil, Pantea
Gohari, Ahmad R
Ajani, Yousef
Hosseini, Rohollah
Hadjiakhoondi, Abbass
Saeidnia, Soodabeh
author_sort Sarkhail, Parisa
collection PubMed
description BACKGROUND: The genus Phlomis (Lamiaceae) is introduced by its valuable medicinal species, of which 17 species are growing wildly and ten of them are exclusively endemic of Iran. The main phytochemical characteristic of this genus is presence of iridoid glycosides including ipolamide, auroside, lamiide and also phenylethanoids such as verbascoside (acetoside) found in Lamiales order. Due to the broad range of biological and pharmacological activities of verbascoside and lack of any report on quantification of this compound within Iranian species of Phlomis, we conducted a research to achieve two main goals, finding a genetic biodiversity by RAPD (Randomly Amplified Polymorphic DNA), as well as detecting and quantifying verbascoside in nine species of Phlomis growing wildly in Iran. RESULTS: The results showed that various samples of P.olivieri possess different genetic distances from each other. Also, various species of P.olivieri display close relationships to P.anisodonta and P. persica. Phytoanalysis of Phlomis species by means of TLC scanner using verbascoside as a phytochemical marker showed that the highest concentration of verbascoside was found in P. anisodonta, however, P. bruguieri and P. olivieri (from Mazandaran) were in the second and third places. Interestingly, the lowest concentration of verbascoside was detected in P. olivieri (from Azerbayjan), exhibiting the effect of various growing areas and conditions on the measured levels of this compound. CONCLUSIONS: verbascoside can be found in various species of Iranian Phlomis, of which P. anisodonta, P. bruguieri and P. olivieri might be the best choices. In addition, although the concentration of verbascoside in these plants may be affected by the growing areas and conditions, there are a good agreement between genetic relations and verbascoside levels.
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spelling pubmed-39981862014-05-08 Quantification of verbascoside in medicinal species of Phlomis and their genetic relationships Sarkhail, Parisa Nikan, Marjan Sarkheil, Pantea Gohari, Ahmad R Ajani, Yousef Hosseini, Rohollah Hadjiakhoondi, Abbass Saeidnia, Soodabeh Daru Research Article BACKGROUND: The genus Phlomis (Lamiaceae) is introduced by its valuable medicinal species, of which 17 species are growing wildly and ten of them are exclusively endemic of Iran. The main phytochemical characteristic of this genus is presence of iridoid glycosides including ipolamide, auroside, lamiide and also phenylethanoids such as verbascoside (acetoside) found in Lamiales order. Due to the broad range of biological and pharmacological activities of verbascoside and lack of any report on quantification of this compound within Iranian species of Phlomis, we conducted a research to achieve two main goals, finding a genetic biodiversity by RAPD (Randomly Amplified Polymorphic DNA), as well as detecting and quantifying verbascoside in nine species of Phlomis growing wildly in Iran. RESULTS: The results showed that various samples of P.olivieri possess different genetic distances from each other. Also, various species of P.olivieri display close relationships to P.anisodonta and P. persica. Phytoanalysis of Phlomis species by means of TLC scanner using verbascoside as a phytochemical marker showed that the highest concentration of verbascoside was found in P. anisodonta, however, P. bruguieri and P. olivieri (from Mazandaran) were in the second and third places. Interestingly, the lowest concentration of verbascoside was detected in P. olivieri (from Azerbayjan), exhibiting the effect of various growing areas and conditions on the measured levels of this compound. CONCLUSIONS: verbascoside can be found in various species of Iranian Phlomis, of which P. anisodonta, P. bruguieri and P. olivieri might be the best choices. In addition, although the concentration of verbascoside in these plants may be affected by the growing areas and conditions, there are a good agreement between genetic relations and verbascoside levels. BioMed Central 2014-03-20 /pmc/articles/PMC3998186/ /pubmed/24650578 http://dx.doi.org/10.1186/2008-2231-22-32 Text en Copyright © 2014 Sarkhail et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Sarkhail, Parisa
Nikan, Marjan
Sarkheil, Pantea
Gohari, Ahmad R
Ajani, Yousef
Hosseini, Rohollah
Hadjiakhoondi, Abbass
Saeidnia, Soodabeh
Quantification of verbascoside in medicinal species of Phlomis and their genetic relationships
title Quantification of verbascoside in medicinal species of Phlomis and their genetic relationships
title_full Quantification of verbascoside in medicinal species of Phlomis and their genetic relationships
title_fullStr Quantification of verbascoside in medicinal species of Phlomis and their genetic relationships
title_full_unstemmed Quantification of verbascoside in medicinal species of Phlomis and their genetic relationships
title_short Quantification of verbascoside in medicinal species of Phlomis and their genetic relationships
title_sort quantification of verbascoside in medicinal species of phlomis and their genetic relationships
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998186/
https://www.ncbi.nlm.nih.gov/pubmed/24650578
http://dx.doi.org/10.1186/2008-2231-22-32
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