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Cell Wall Composition of Bacillus subtilis Changes as a Function of pH and Zn(2+) Exposure: Insights from Cryo-XPS Measurements

[Image: see text] Bacteria play an important role in the biogeochemical cycling of metals in the environment. Consequently, there is an interest to understand how the bacterial surfaces interact with metals in solution and how this affects the bacterial surface. In this work we have used a surface-s...

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Autores principales: Ramstedt, Madeleine, Leone, Laura, Persson, Per, Shchukarev, Andrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998515/
https://www.ncbi.nlm.nih.gov/pubmed/24670213
http://dx.doi.org/10.1021/la5002573
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author Ramstedt, Madeleine
Leone, Laura
Persson, Per
Shchukarev, Andrey
author_facet Ramstedt, Madeleine
Leone, Laura
Persson, Per
Shchukarev, Andrey
author_sort Ramstedt, Madeleine
collection PubMed
description [Image: see text] Bacteria play an important role in the biogeochemical cycling of metals in the environment. Consequently, there is an interest to understand how the bacterial surfaces interact with metals in solution and how this affects the bacterial surface. In this work we have used a surface-sensitive analysis technique, cryogenic X-ray photoelectron spectroscopy (cryo-XPS), to monitor the surface of Bacillus subtilis cells as a function of pH and Zn(2+) content in saline solution. The objective of the study was twofold: (1) to investigate the agreement between two data treatment methods for XPS, as well as investigate to what extent sample pretreatment may influence XPS data of bacterial samples, and (2) to characterize how the surface chemistry of bacterial cells is influenced by different external conditions. (1) It was found that the two data treatment methods gave rise to comparable results. However, identical samples analyzed fast-frozen or dry exhibited larger differences in surface chemistry, indicating that sample pretreatment can to large extents influence the obtained surface composition of bacterial samples. (2) The bacterial cell wall (in fast-frozen samples) undergoes dramatic compositional changes with pH and with Zn(2+) exposure. The compositional changes are interpreted as an adaptive metal resistance response changing the biochemical composition of the bacterial cell wall. These results have implications for how adsorption processes at the surface of bacterial cells are analyzed, understood, modeled, and predicted.
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spelling pubmed-39985152014-04-28 Cell Wall Composition of Bacillus subtilis Changes as a Function of pH and Zn(2+) Exposure: Insights from Cryo-XPS Measurements Ramstedt, Madeleine Leone, Laura Persson, Per Shchukarev, Andrey Langmuir [Image: see text] Bacteria play an important role in the biogeochemical cycling of metals in the environment. Consequently, there is an interest to understand how the bacterial surfaces interact with metals in solution and how this affects the bacterial surface. In this work we have used a surface-sensitive analysis technique, cryogenic X-ray photoelectron spectroscopy (cryo-XPS), to monitor the surface of Bacillus subtilis cells as a function of pH and Zn(2+) content in saline solution. The objective of the study was twofold: (1) to investigate the agreement between two data treatment methods for XPS, as well as investigate to what extent sample pretreatment may influence XPS data of bacterial samples, and (2) to characterize how the surface chemistry of bacterial cells is influenced by different external conditions. (1) It was found that the two data treatment methods gave rise to comparable results. However, identical samples analyzed fast-frozen or dry exhibited larger differences in surface chemistry, indicating that sample pretreatment can to large extents influence the obtained surface composition of bacterial samples. (2) The bacterial cell wall (in fast-frozen samples) undergoes dramatic compositional changes with pH and with Zn(2+) exposure. The compositional changes are interpreted as an adaptive metal resistance response changing the biochemical composition of the bacterial cell wall. These results have implications for how adsorption processes at the surface of bacterial cells are analyzed, understood, modeled, and predicted. American Chemical Society 2014-03-26 2014-04-22 /pmc/articles/PMC3998515/ /pubmed/24670213 http://dx.doi.org/10.1021/la5002573 Text en Copyright © 2014 American Chemical Society Terms of Use CC-BY (http://pubs.acs.org/page/policy/authorchoice_ccby_termsofuse.html)
spellingShingle Ramstedt, Madeleine
Leone, Laura
Persson, Per
Shchukarev, Andrey
Cell Wall Composition of Bacillus subtilis Changes as a Function of pH and Zn(2+) Exposure: Insights from Cryo-XPS Measurements
title Cell Wall Composition of Bacillus subtilis Changes as a Function of pH and Zn(2+) Exposure: Insights from Cryo-XPS Measurements
title_full Cell Wall Composition of Bacillus subtilis Changes as a Function of pH and Zn(2+) Exposure: Insights from Cryo-XPS Measurements
title_fullStr Cell Wall Composition of Bacillus subtilis Changes as a Function of pH and Zn(2+) Exposure: Insights from Cryo-XPS Measurements
title_full_unstemmed Cell Wall Composition of Bacillus subtilis Changes as a Function of pH and Zn(2+) Exposure: Insights from Cryo-XPS Measurements
title_short Cell Wall Composition of Bacillus subtilis Changes as a Function of pH and Zn(2+) Exposure: Insights from Cryo-XPS Measurements
title_sort cell wall composition of bacillus subtilis changes as a function of ph and zn(2+) exposure: insights from cryo-xps measurements
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998515/
https://www.ncbi.nlm.nih.gov/pubmed/24670213
http://dx.doi.org/10.1021/la5002573
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