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Both contractile axial and lateral traction force dynamics drive amoeboid cell motility

Chemotaxing Dictyostelium discoideum cells adapt their morphology and migration speed in response to intrinsic and extrinsic cues. Using Fourier traction force microscopy, we measured the spatiotemporal evolution of shape and traction stresses and constructed traction tension kymographs to analyze c...

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Autores principales: Bastounis, Effie, Meili, Ruedi, Álvarez-González, Begoña, Francois, Joshua, del Álamo, Juan C., Firtel, Richard A., Lasheras, Juan C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998796/
https://www.ncbi.nlm.nih.gov/pubmed/24637328
http://dx.doi.org/10.1083/jcb.201307106
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author Bastounis, Effie
Meili, Ruedi
Álvarez-González, Begoña
Francois, Joshua
del Álamo, Juan C.
Firtel, Richard A.
Lasheras, Juan C.
author_facet Bastounis, Effie
Meili, Ruedi
Álvarez-González, Begoña
Francois, Joshua
del Álamo, Juan C.
Firtel, Richard A.
Lasheras, Juan C.
author_sort Bastounis, Effie
collection PubMed
description Chemotaxing Dictyostelium discoideum cells adapt their morphology and migration speed in response to intrinsic and extrinsic cues. Using Fourier traction force microscopy, we measured the spatiotemporal evolution of shape and traction stresses and constructed traction tension kymographs to analyze cell motility as a function of the dynamics of the cell’s mechanically active traction adhesions. We show that wild-type cells migrate in a step-wise fashion, mainly forming stationary traction adhesions along their anterior–posterior axes and exerting strong contractile axial forces. We demonstrate that lateral forces are also important for motility, especially for migration on highly adhesive substrates. Analysis of two mutant strains lacking distinct actin cross-linkers (mhcA(−) and abp120(−) cells) on normal and highly adhesive substrates supports a key role for lateral contractions in amoeboid cell motility, whereas the differences in their traction adhesion dynamics suggest that these two strains use distinct mechanisms to achieve migration. Finally, we provide evidence that the above patterns of migration may be conserved in mammalian amoeboid cells.
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spelling pubmed-39987962014-09-17 Both contractile axial and lateral traction force dynamics drive amoeboid cell motility Bastounis, Effie Meili, Ruedi Álvarez-González, Begoña Francois, Joshua del Álamo, Juan C. Firtel, Richard A. Lasheras, Juan C. J Cell Biol Research Articles Chemotaxing Dictyostelium discoideum cells adapt their morphology and migration speed in response to intrinsic and extrinsic cues. Using Fourier traction force microscopy, we measured the spatiotemporal evolution of shape and traction stresses and constructed traction tension kymographs to analyze cell motility as a function of the dynamics of the cell’s mechanically active traction adhesions. We show that wild-type cells migrate in a step-wise fashion, mainly forming stationary traction adhesions along their anterior–posterior axes and exerting strong contractile axial forces. We demonstrate that lateral forces are also important for motility, especially for migration on highly adhesive substrates. Analysis of two mutant strains lacking distinct actin cross-linkers (mhcA(−) and abp120(−) cells) on normal and highly adhesive substrates supports a key role for lateral contractions in amoeboid cell motility, whereas the differences in their traction adhesion dynamics suggest that these two strains use distinct mechanisms to achieve migration. Finally, we provide evidence that the above patterns of migration may be conserved in mammalian amoeboid cells. The Rockefeller University Press 2014-03-17 /pmc/articles/PMC3998796/ /pubmed/24637328 http://dx.doi.org/10.1083/jcb.201307106 Text en © 2014 Bastounis et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Bastounis, Effie
Meili, Ruedi
Álvarez-González, Begoña
Francois, Joshua
del Álamo, Juan C.
Firtel, Richard A.
Lasheras, Juan C.
Both contractile axial and lateral traction force dynamics drive amoeboid cell motility
title Both contractile axial and lateral traction force dynamics drive amoeboid cell motility
title_full Both contractile axial and lateral traction force dynamics drive amoeboid cell motility
title_fullStr Both contractile axial and lateral traction force dynamics drive amoeboid cell motility
title_full_unstemmed Both contractile axial and lateral traction force dynamics drive amoeboid cell motility
title_short Both contractile axial and lateral traction force dynamics drive amoeboid cell motility
title_sort both contractile axial and lateral traction force dynamics drive amoeboid cell motility
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998796/
https://www.ncbi.nlm.nih.gov/pubmed/24637328
http://dx.doi.org/10.1083/jcb.201307106
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