Cargando…
Synthesis and in vitro antineoplastic evaluation of silver nanoparticles mediated by Agrimoniae herba extract
A rapid synthesis of silver nanoparticles (AgNPs) using Agrimoniae herba extract as reducing agent and stabilizer (A. herba-conjugated AgNPs [AH-AgNPs]) were designed, characterized, and evaluated for antitumor therapy feasibility. In this study, critical factors in the preparation of silver nanopar...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998862/ https://www.ncbi.nlm.nih.gov/pubmed/24790429 http://dx.doi.org/10.2147/IJN.S58732 |
_version_ | 1782313424233955328 |
---|---|
author | Qu, Ding Sun, Wenjie Chen, Yan Zhou, Jing Liu, Congyan |
author_facet | Qu, Ding Sun, Wenjie Chen, Yan Zhou, Jing Liu, Congyan |
author_sort | Qu, Ding |
collection | PubMed |
description | A rapid synthesis of silver nanoparticles (AgNPs) using Agrimoniae herba extract as reducing agent and stabilizer (A. herba-conjugated AgNPs [AH-AgNPs]) were designed, characterized, and evaluated for antitumor therapy feasibility. In this study, critical factors in the preparation of silver nanoparticles, including extraction time, reaction temperature, the concentration of AgNO(3), and A. herba extract amount, were investigated using ultraviolet-visible spectroscopy. AH-AgNPs with well-defined spherical shape, homogeneous distributional small size (30.34 nm), narrow polydispersity index (0.142), and high negative zeta potential (−36.8 mV) were observed by transmission electron microscopy and dynamic light scattering. Furthermore, the results of X-ray diffraction and Fourier-transform infrared spectroscopy further indicated successful preparation of AH-AgNPs. Acceptable long-term storage stability of AH-AgNPs was also confirmed. More importantly, AH-AgNPs displayed significantly higher antiproliferative effect against a human lung carcinoma cell line (A549 cells) compared with A. herba extract and bare AgNPs prepared by sodium citrate. The half-maximal inhibitory concentrations of AH-AgNPs, bare AgNPs, and A. herba extract were 38.13 μg · mL(−1), 184.87 μg · mL(−1), and 1.147 × 10(4) μg · mL(−1), respectively. It is suggested that AH-AgNPs exhibit a strong antineoplastic effect on A549 cells, pointing to feasibility of antitumor treatment in the future. |
format | Online Article Text |
id | pubmed-3998862 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-39988622014-04-30 Synthesis and in vitro antineoplastic evaluation of silver nanoparticles mediated by Agrimoniae herba extract Qu, Ding Sun, Wenjie Chen, Yan Zhou, Jing Liu, Congyan Int J Nanomedicine Original Research A rapid synthesis of silver nanoparticles (AgNPs) using Agrimoniae herba extract as reducing agent and stabilizer (A. herba-conjugated AgNPs [AH-AgNPs]) were designed, characterized, and evaluated for antitumor therapy feasibility. In this study, critical factors in the preparation of silver nanoparticles, including extraction time, reaction temperature, the concentration of AgNO(3), and A. herba extract amount, were investigated using ultraviolet-visible spectroscopy. AH-AgNPs with well-defined spherical shape, homogeneous distributional small size (30.34 nm), narrow polydispersity index (0.142), and high negative zeta potential (−36.8 mV) were observed by transmission electron microscopy and dynamic light scattering. Furthermore, the results of X-ray diffraction and Fourier-transform infrared spectroscopy further indicated successful preparation of AH-AgNPs. Acceptable long-term storage stability of AH-AgNPs was also confirmed. More importantly, AH-AgNPs displayed significantly higher antiproliferative effect against a human lung carcinoma cell line (A549 cells) compared with A. herba extract and bare AgNPs prepared by sodium citrate. The half-maximal inhibitory concentrations of AH-AgNPs, bare AgNPs, and A. herba extract were 38.13 μg · mL(−1), 184.87 μg · mL(−1), and 1.147 × 10(4) μg · mL(−1), respectively. It is suggested that AH-AgNPs exhibit a strong antineoplastic effect on A549 cells, pointing to feasibility of antitumor treatment in the future. Dove Medical Press 2014-04-15 /pmc/articles/PMC3998862/ /pubmed/24790429 http://dx.doi.org/10.2147/IJN.S58732 Text en © 2014 Qu et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Qu, Ding Sun, Wenjie Chen, Yan Zhou, Jing Liu, Congyan Synthesis and in vitro antineoplastic evaluation of silver nanoparticles mediated by Agrimoniae herba extract |
title | Synthesis and in vitro antineoplastic evaluation of silver nanoparticles mediated by Agrimoniae herba extract |
title_full | Synthesis and in vitro antineoplastic evaluation of silver nanoparticles mediated by Agrimoniae herba extract |
title_fullStr | Synthesis and in vitro antineoplastic evaluation of silver nanoparticles mediated by Agrimoniae herba extract |
title_full_unstemmed | Synthesis and in vitro antineoplastic evaluation of silver nanoparticles mediated by Agrimoniae herba extract |
title_short | Synthesis and in vitro antineoplastic evaluation of silver nanoparticles mediated by Agrimoniae herba extract |
title_sort | synthesis and in vitro antineoplastic evaluation of silver nanoparticles mediated by agrimoniae herba extract |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998862/ https://www.ncbi.nlm.nih.gov/pubmed/24790429 http://dx.doi.org/10.2147/IJN.S58732 |
work_keys_str_mv | AT quding synthesisandinvitroantineoplasticevaluationofsilvernanoparticlesmediatedbyagrimoniaeherbaextract AT sunwenjie synthesisandinvitroantineoplasticevaluationofsilvernanoparticlesmediatedbyagrimoniaeherbaextract AT chenyan synthesisandinvitroantineoplasticevaluationofsilvernanoparticlesmediatedbyagrimoniaeherbaextract AT zhoujing synthesisandinvitroantineoplasticevaluationofsilvernanoparticlesmediatedbyagrimoniaeherbaextract AT liucongyan synthesisandinvitroantineoplasticevaluationofsilvernanoparticlesmediatedbyagrimoniaeherbaextract |