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Antigen-Specific Memory B-cell Responses to Enterotoxigenic Escherichia coli Infection in Bangladeshi Adults

BACKGROUND: Multiple infections with diverse enterotoxigenic E. coli (ETEC) strains lead to broad spectrum protection against ETEC diarrhea. However, the precise mechanism of protection against ETEC infection is still unknown. Therefore, memory B cell responses and affinity maturation of antibodies...

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Autores principales: Alam, Mohammad Murshid, Aktar, Amena, Afrin, Sadia, Rahman, Mohammad Arif, Aktar, Sarmin, Uddin, Taher, Rahman, M. Arifur, Mahbuba, Deena Al, Chowdhury, Fahima, Khan, Ashraful Islam, Bhuiyan, Taufiqur Rahman, Begum, Yasmin Ara, Ryan, Edward T., Calderwood, Stephen B., Svennerholm, Ann-Mari, Qadri, Firdausi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998937/
https://www.ncbi.nlm.nih.gov/pubmed/24762744
http://dx.doi.org/10.1371/journal.pntd.0002822
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author Alam, Mohammad Murshid
Aktar, Amena
Afrin, Sadia
Rahman, Mohammad Arif
Aktar, Sarmin
Uddin, Taher
Rahman, M. Arifur
Mahbuba, Deena Al
Chowdhury, Fahima
Khan, Ashraful Islam
Bhuiyan, Taufiqur Rahman
Begum, Yasmin Ara
Ryan, Edward T.
Calderwood, Stephen B.
Svennerholm, Ann-Mari
Qadri, Firdausi
author_facet Alam, Mohammad Murshid
Aktar, Amena
Afrin, Sadia
Rahman, Mohammad Arif
Aktar, Sarmin
Uddin, Taher
Rahman, M. Arifur
Mahbuba, Deena Al
Chowdhury, Fahima
Khan, Ashraful Islam
Bhuiyan, Taufiqur Rahman
Begum, Yasmin Ara
Ryan, Edward T.
Calderwood, Stephen B.
Svennerholm, Ann-Mari
Qadri, Firdausi
author_sort Alam, Mohammad Murshid
collection PubMed
description BACKGROUND: Multiple infections with diverse enterotoxigenic E. coli (ETEC) strains lead to broad spectrum protection against ETEC diarrhea. However, the precise mechanism of protection against ETEC infection is still unknown. Therefore, memory B cell responses and affinity maturation of antibodies to the specific ETEC antigens might be important to understand the mechanism of protection. METHODOLOGY: In this study, we investigated the heat labile toxin B subunit (LTB) and colonization factor antigens (CFA/I and CS6) specific IgA and IgG memory B cell responses in Bangladeshi adults (n = 52) who were infected with ETEC. We also investigated the avidity of IgA and IgG antibodies that developed after infection to these antigens. PRINCIPAL FINDINGS: Patients infected with ETEC expressing LT or LT+heat stable toxin (ST) and CFA/I group or CS6 colonization factors developed LTB, CFA/I or CS6 specific memory B cell responses at day 30 after infection. Similarly, these patients developed high avidity IgA and IgG antibodies to LTB, CFA/I or CS6 at day 7 that remained significantly elevated at day 30 when compared to the avidity of these specific antibodies at the acute stage of infection (day 2). The memory B cell responses, antibody avidity and other immune responses to CFA/I not only developed in patients infected with ETEC expressing CFA/I but also in those infected with ETEC expressing CFA/I cross-reacting epitopes. We also detected a significant positive correlation of LTB, CFA/I and CS6 specific memory B cell responses with the corresponding increase in antibody avidity. CONCLUSION: This study demonstrates that natural infection with ETEC induces memory B cells and high avidity antibodies to LTB and colonization factor CFA/I and CS6 antigens that could mediate anamnestic responses on re-exposure to ETEC and may help in understanding the requirements to design an effective vaccination strategies.
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spelling pubmed-39989372014-04-29 Antigen-Specific Memory B-cell Responses to Enterotoxigenic Escherichia coli Infection in Bangladeshi Adults Alam, Mohammad Murshid Aktar, Amena Afrin, Sadia Rahman, Mohammad Arif Aktar, Sarmin Uddin, Taher Rahman, M. Arifur Mahbuba, Deena Al Chowdhury, Fahima Khan, Ashraful Islam Bhuiyan, Taufiqur Rahman Begum, Yasmin Ara Ryan, Edward T. Calderwood, Stephen B. Svennerholm, Ann-Mari Qadri, Firdausi PLoS Negl Trop Dis Research Article BACKGROUND: Multiple infections with diverse enterotoxigenic E. coli (ETEC) strains lead to broad spectrum protection against ETEC diarrhea. However, the precise mechanism of protection against ETEC infection is still unknown. Therefore, memory B cell responses and affinity maturation of antibodies to the specific ETEC antigens might be important to understand the mechanism of protection. METHODOLOGY: In this study, we investigated the heat labile toxin B subunit (LTB) and colonization factor antigens (CFA/I and CS6) specific IgA and IgG memory B cell responses in Bangladeshi adults (n = 52) who were infected with ETEC. We also investigated the avidity of IgA and IgG antibodies that developed after infection to these antigens. PRINCIPAL FINDINGS: Patients infected with ETEC expressing LT or LT+heat stable toxin (ST) and CFA/I group or CS6 colonization factors developed LTB, CFA/I or CS6 specific memory B cell responses at day 30 after infection. Similarly, these patients developed high avidity IgA and IgG antibodies to LTB, CFA/I or CS6 at day 7 that remained significantly elevated at day 30 when compared to the avidity of these specific antibodies at the acute stage of infection (day 2). The memory B cell responses, antibody avidity and other immune responses to CFA/I not only developed in patients infected with ETEC expressing CFA/I but also in those infected with ETEC expressing CFA/I cross-reacting epitopes. We also detected a significant positive correlation of LTB, CFA/I and CS6 specific memory B cell responses with the corresponding increase in antibody avidity. CONCLUSION: This study demonstrates that natural infection with ETEC induces memory B cells and high avidity antibodies to LTB and colonization factor CFA/I and CS6 antigens that could mediate anamnestic responses on re-exposure to ETEC and may help in understanding the requirements to design an effective vaccination strategies. Public Library of Science 2014-04-24 /pmc/articles/PMC3998937/ /pubmed/24762744 http://dx.doi.org/10.1371/journal.pntd.0002822 Text en © 2014 Alam et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Alam, Mohammad Murshid
Aktar, Amena
Afrin, Sadia
Rahman, Mohammad Arif
Aktar, Sarmin
Uddin, Taher
Rahman, M. Arifur
Mahbuba, Deena Al
Chowdhury, Fahima
Khan, Ashraful Islam
Bhuiyan, Taufiqur Rahman
Begum, Yasmin Ara
Ryan, Edward T.
Calderwood, Stephen B.
Svennerholm, Ann-Mari
Qadri, Firdausi
Antigen-Specific Memory B-cell Responses to Enterotoxigenic Escherichia coli Infection in Bangladeshi Adults
title Antigen-Specific Memory B-cell Responses to Enterotoxigenic Escherichia coli Infection in Bangladeshi Adults
title_full Antigen-Specific Memory B-cell Responses to Enterotoxigenic Escherichia coli Infection in Bangladeshi Adults
title_fullStr Antigen-Specific Memory B-cell Responses to Enterotoxigenic Escherichia coli Infection in Bangladeshi Adults
title_full_unstemmed Antigen-Specific Memory B-cell Responses to Enterotoxigenic Escherichia coli Infection in Bangladeshi Adults
title_short Antigen-Specific Memory B-cell Responses to Enterotoxigenic Escherichia coli Infection in Bangladeshi Adults
title_sort antigen-specific memory b-cell responses to enterotoxigenic escherichia coli infection in bangladeshi adults
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3998937/
https://www.ncbi.nlm.nih.gov/pubmed/24762744
http://dx.doi.org/10.1371/journal.pntd.0002822
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