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Widespread Occurrence of Expressed Fungal Secretory Peroxidases in Forest Soils

Fungal secretory peroxidases mediate fundamental ecological functions in the conversion and degradation of plant biomass. Many of these enzymes have strong oxidizing activities towards aromatic compounds and are involved in the degradation of plant cell wall (lignin) and humus. They comprise three m...

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Autores principales: Kellner, Harald, Luis, Patricia, Pecyna, Marek J., Barbi, Florian, Kapturska, Danuta, Krüger, Dirk, Zak, Donald R., Marmeisse, Roland, Vandenbol, Micheline, Hofrichter, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999038/
https://www.ncbi.nlm.nih.gov/pubmed/24763280
http://dx.doi.org/10.1371/journal.pone.0095557
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author Kellner, Harald
Luis, Patricia
Pecyna, Marek J.
Barbi, Florian
Kapturska, Danuta
Krüger, Dirk
Zak, Donald R.
Marmeisse, Roland
Vandenbol, Micheline
Hofrichter, Martin
author_facet Kellner, Harald
Luis, Patricia
Pecyna, Marek J.
Barbi, Florian
Kapturska, Danuta
Krüger, Dirk
Zak, Donald R.
Marmeisse, Roland
Vandenbol, Micheline
Hofrichter, Martin
author_sort Kellner, Harald
collection PubMed
description Fungal secretory peroxidases mediate fundamental ecological functions in the conversion and degradation of plant biomass. Many of these enzymes have strong oxidizing activities towards aromatic compounds and are involved in the degradation of plant cell wall (lignin) and humus. They comprise three major groups: class II peroxidases (including lignin peroxidase, manganese peroxidase, versatile peroxidase and generic peroxidase), dye-decolorizing peroxidases, and heme-thiolate peroxidases (e.g. unspecific/aromatic peroxygenase, chloroperoxidase). Here, we have repeatedly observed a widespread expression of all major peroxidase groups in leaf and needle litter across a range of forest ecosystems (e.g. Fagus, Picea, Acer, Quercus, and Populus spp.), which are widespread in Europe and North America. Manganese peroxidases and unspecific peroxygenases were found expressed in all nine investigated forest sites, and dye-decolorizing peroxidases were observed in five of the nine sites, thereby indicating biological significance of these enzymes for fungal physiology and ecosystem processes. Transcripts of selected secretory peroxidase genes were also analyzed in pure cultures of several litter-decomposing species and other fungi. Using this information, we were able to match, in environmental litter samples, two manganese peroxidase sequences to Mycena galopus and Mycena epipterygia and one unspecific peroxygenase transcript to Mycena galopus, suggesting an important role of this litter- and coarse woody debris-dwelling genus in the disintegration and transformation of litter aromatics and organic matter formation.
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spelling pubmed-39990382014-04-29 Widespread Occurrence of Expressed Fungal Secretory Peroxidases in Forest Soils Kellner, Harald Luis, Patricia Pecyna, Marek J. Barbi, Florian Kapturska, Danuta Krüger, Dirk Zak, Donald R. Marmeisse, Roland Vandenbol, Micheline Hofrichter, Martin PLoS One Research Article Fungal secretory peroxidases mediate fundamental ecological functions in the conversion and degradation of plant biomass. Many of these enzymes have strong oxidizing activities towards aromatic compounds and are involved in the degradation of plant cell wall (lignin) and humus. They comprise three major groups: class II peroxidases (including lignin peroxidase, manganese peroxidase, versatile peroxidase and generic peroxidase), dye-decolorizing peroxidases, and heme-thiolate peroxidases (e.g. unspecific/aromatic peroxygenase, chloroperoxidase). Here, we have repeatedly observed a widespread expression of all major peroxidase groups in leaf and needle litter across a range of forest ecosystems (e.g. Fagus, Picea, Acer, Quercus, and Populus spp.), which are widespread in Europe and North America. Manganese peroxidases and unspecific peroxygenases were found expressed in all nine investigated forest sites, and dye-decolorizing peroxidases were observed in five of the nine sites, thereby indicating biological significance of these enzymes for fungal physiology and ecosystem processes. Transcripts of selected secretory peroxidase genes were also analyzed in pure cultures of several litter-decomposing species and other fungi. Using this information, we were able to match, in environmental litter samples, two manganese peroxidase sequences to Mycena galopus and Mycena epipterygia and one unspecific peroxygenase transcript to Mycena galopus, suggesting an important role of this litter- and coarse woody debris-dwelling genus in the disintegration and transformation of litter aromatics and organic matter formation. Public Library of Science 2014-04-24 /pmc/articles/PMC3999038/ /pubmed/24763280 http://dx.doi.org/10.1371/journal.pone.0095557 Text en © 2014 Kellner et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kellner, Harald
Luis, Patricia
Pecyna, Marek J.
Barbi, Florian
Kapturska, Danuta
Krüger, Dirk
Zak, Donald R.
Marmeisse, Roland
Vandenbol, Micheline
Hofrichter, Martin
Widespread Occurrence of Expressed Fungal Secretory Peroxidases in Forest Soils
title Widespread Occurrence of Expressed Fungal Secretory Peroxidases in Forest Soils
title_full Widespread Occurrence of Expressed Fungal Secretory Peroxidases in Forest Soils
title_fullStr Widespread Occurrence of Expressed Fungal Secretory Peroxidases in Forest Soils
title_full_unstemmed Widespread Occurrence of Expressed Fungal Secretory Peroxidases in Forest Soils
title_short Widespread Occurrence of Expressed Fungal Secretory Peroxidases in Forest Soils
title_sort widespread occurrence of expressed fungal secretory peroxidases in forest soils
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999038/
https://www.ncbi.nlm.nih.gov/pubmed/24763280
http://dx.doi.org/10.1371/journal.pone.0095557
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