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Plasmodium genetic loci linked to host cytokine and chemokine responses
Both host and parasite factors contribute to disease severity of malaria infection; however, the molecular mechanisms responsible for the disease and the host-parasite interactions involved remain largely unresolved. To investigate effects of parasite factors on host immune responses and pathogenesi...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999244/ https://www.ncbi.nlm.nih.gov/pubmed/24452266 http://dx.doi.org/10.1038/gene.2013.74 |
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author | Pattaradilokrat, Sittiporn Li, Jian Wu, Jian Qi, Yanwei Eastman, Richard T. Zilversmit, Martine Nair, Sethu C. Huaman, Maria Cecilia Quinones, Mariam Jiang, Hongying Li, Na Zhu, Jun Zhao, Keji Kaneko, Osamu Long, Carole A. Su, Xin-zhuan |
author_facet | Pattaradilokrat, Sittiporn Li, Jian Wu, Jian Qi, Yanwei Eastman, Richard T. Zilversmit, Martine Nair, Sethu C. Huaman, Maria Cecilia Quinones, Mariam Jiang, Hongying Li, Na Zhu, Jun Zhao, Keji Kaneko, Osamu Long, Carole A. Su, Xin-zhuan |
author_sort | Pattaradilokrat, Sittiporn |
collection | PubMed |
description | Both host and parasite factors contribute to disease severity of malaria infection; however, the molecular mechanisms responsible for the disease and the host-parasite interactions involved remain largely unresolved. To investigate effects of parasite factors on host immune responses and pathogenesis, we measured levels of plasma cytokines/chemokines (CC) and growth rates in mice infected with two Plasmodium yoelii strains having different virulence phenotypes and in progeny from a genetic cross of the two parasites. Quantitative trait loci (QTL) analysis linked levels of many CCs, particularly IL-1β, IP-10, IFN-γ, MCP-1, and MIG, and early parasite growth rate to loci on multiple parasite chromosomes, including chromosomes 7, 9, 10, 12, and 13. Comparison of the genome sequences spanning the mapped loci revealed various candidate genes. The loci on chromosome 7 and 13 had significant (p < 0.005) additive effects on IL-1β, IL-5, and IP-10 responses, and the chromosome 9 and 12 loci had significant (p = 0.017) interaction. Infection of knockout mice showed critical roles of MCP-1 and IL-10 in parasitemia control and host mortality. These results provide important information for better understanding of malaria pathogenesis and can be used to examine the role of these factors in human malaria infection. |
format | Online Article Text |
id | pubmed-3999244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
record_format | MEDLINE/PubMed |
spelling | pubmed-39992442014-10-01 Plasmodium genetic loci linked to host cytokine and chemokine responses Pattaradilokrat, Sittiporn Li, Jian Wu, Jian Qi, Yanwei Eastman, Richard T. Zilversmit, Martine Nair, Sethu C. Huaman, Maria Cecilia Quinones, Mariam Jiang, Hongying Li, Na Zhu, Jun Zhao, Keji Kaneko, Osamu Long, Carole A. Su, Xin-zhuan Genes Immun Article Both host and parasite factors contribute to disease severity of malaria infection; however, the molecular mechanisms responsible for the disease and the host-parasite interactions involved remain largely unresolved. To investigate effects of parasite factors on host immune responses and pathogenesis, we measured levels of plasma cytokines/chemokines (CC) and growth rates in mice infected with two Plasmodium yoelii strains having different virulence phenotypes and in progeny from a genetic cross of the two parasites. Quantitative trait loci (QTL) analysis linked levels of many CCs, particularly IL-1β, IP-10, IFN-γ, MCP-1, and MIG, and early parasite growth rate to loci on multiple parasite chromosomes, including chromosomes 7, 9, 10, 12, and 13. Comparison of the genome sequences spanning the mapped loci revealed various candidate genes. The loci on chromosome 7 and 13 had significant (p < 0.005) additive effects on IL-1β, IL-5, and IP-10 responses, and the chromosome 9 and 12 loci had significant (p = 0.017) interaction. Infection of knockout mice showed critical roles of MCP-1 and IL-10 in parasitemia control and host mortality. These results provide important information for better understanding of malaria pathogenesis and can be used to examine the role of these factors in human malaria infection. 2014-01-23 2014 /pmc/articles/PMC3999244/ /pubmed/24452266 http://dx.doi.org/10.1038/gene.2013.74 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Pattaradilokrat, Sittiporn Li, Jian Wu, Jian Qi, Yanwei Eastman, Richard T. Zilversmit, Martine Nair, Sethu C. Huaman, Maria Cecilia Quinones, Mariam Jiang, Hongying Li, Na Zhu, Jun Zhao, Keji Kaneko, Osamu Long, Carole A. Su, Xin-zhuan Plasmodium genetic loci linked to host cytokine and chemokine responses |
title | Plasmodium genetic loci linked to host cytokine and chemokine responses |
title_full | Plasmodium genetic loci linked to host cytokine and chemokine responses |
title_fullStr | Plasmodium genetic loci linked to host cytokine and chemokine responses |
title_full_unstemmed | Plasmodium genetic loci linked to host cytokine and chemokine responses |
title_short | Plasmodium genetic loci linked to host cytokine and chemokine responses |
title_sort | plasmodium genetic loci linked to host cytokine and chemokine responses |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999244/ https://www.ncbi.nlm.nih.gov/pubmed/24452266 http://dx.doi.org/10.1038/gene.2013.74 |
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