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Evaluation of Propidium Monoazide Real-Time PCR for Early Detection of Viable Mycobacterium tuberculosis in Clinical Respiratory Specimens
BACKGROUND: Conventional acid-fast bacilli (AFB) staining cannot differentiate viable from dead cells. Propidium monoazide (PMA) is a photoreactive DNA-binding dye that inhibits PCR amplification by DNA modification. We evaluated whether PMA real-time PCR is suitable for the early detection of viabl...
Autores principales: | Kim, Young Jin, Lee, Sun Min, Park, Byung Kyu, Kim, Sung Soo, Yi, Jongyoun, Kim, Hyung Hoi, Lee, Eun Yup, Chang, Chulhun Ludgerus |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Laboratory Medicine
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999318/ https://www.ncbi.nlm.nih.gov/pubmed/24790907 http://dx.doi.org/10.3343/alm.2014.34.3.203 |
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