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Evaluation of Propidium Monoazide Real-Time PCR for Early Detection of Viable Mycobacterium tuberculosis in Clinical Respiratory Specimens

BACKGROUND: Conventional acid-fast bacilli (AFB) staining cannot differentiate viable from dead cells. Propidium monoazide (PMA) is a photoreactive DNA-binding dye that inhibits PCR amplification by DNA modification. We evaluated whether PMA real-time PCR is suitable for the early detection of viabl...

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Detalles Bibliográficos
Autores principales: Kim, Young Jin, Lee, Sun Min, Park, Byung Kyu, Kim, Sung Soo, Yi, Jongyoun, Kim, Hyung Hoi, Lee, Eun Yup, Chang, Chulhun Ludgerus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Laboratory Medicine 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999318/
https://www.ncbi.nlm.nih.gov/pubmed/24790907
http://dx.doi.org/10.3343/alm.2014.34.3.203

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