Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells

Dermal papilla (DP) cells are unique regional stem cells of the skin that induce formation of a hair follicle and its regeneration cycle. DP are multipotent stem cells; therefore we supposed that the efficiency of DPC reprogramming could exceed that of dermal fibroblasts reprogramming. We generated...

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Autores principales: Muchkaeva, I. A., Dashinimaev, E. B., Artyuhov, A. S., Myagkova, E. P., Vorotelyak, E. A., Yegorov, Y. Y., Vishnyakova, K. S., Kravchenko, I. E., Chumakov, P. M., Terskikh, V. V., Vasiliev, A. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999465/
https://www.ncbi.nlm.nih.gov/pubmed/24772326
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author Muchkaeva, I. A.
Dashinimaev, E. B.
Artyuhov, A. S.
Myagkova, E. P.
Vorotelyak, E. A.
Yegorov, Y. Y.
Vishnyakova, K. S.
Kravchenko, I. E.
Chumakov, P. M.
Terskikh, V. V.
Vasiliev, A. V.
author_facet Muchkaeva, I. A.
Dashinimaev, E. B.
Artyuhov, A. S.
Myagkova, E. P.
Vorotelyak, E. A.
Yegorov, Y. Y.
Vishnyakova, K. S.
Kravchenko, I. E.
Chumakov, P. M.
Terskikh, V. V.
Vasiliev, A. V.
author_sort Muchkaeva, I. A.
collection PubMed
description Dermal papilla (DP) cells are unique regional stem cells of the skin that induce formation of a hair follicle and its regeneration cycle. DP are multipotent stem cells; therefore we supposed that the efficiency of DPC reprogramming could exceed that of dermal fibroblasts reprogramming. We generated induced pluripotent stem cells from human DP cells using lentiviral transfection with Oct4, Sox2, Klf4, and c-Myc, and cultivation of cells both in a medium supplemented with valproic acid and at a physiological level of oxygen (5%). The efficiency of DP cells reprogramming was ~0.03%, while the efficiency of dermal fibroblast reprogramming under the same conditions was ~0.01%. Therefore, we demonstrated the suitability of DP cells as an alternative source of iPS cells.
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spelling pubmed-39994652014-04-25 Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells Muchkaeva, I. A. Dashinimaev, E. B. Artyuhov, A. S. Myagkova, E. P. Vorotelyak, E. A. Yegorov, Y. Y. Vishnyakova, K. S. Kravchenko, I. E. Chumakov, P. M. Terskikh, V. V. Vasiliev, A. V. Acta Naturae Research Article Dermal papilla (DP) cells are unique regional stem cells of the skin that induce formation of a hair follicle and its regeneration cycle. DP are multipotent stem cells; therefore we supposed that the efficiency of DPC reprogramming could exceed that of dermal fibroblasts reprogramming. We generated induced pluripotent stem cells from human DP cells using lentiviral transfection with Oct4, Sox2, Klf4, and c-Myc, and cultivation of cells both in a medium supplemented with valproic acid and at a physiological level of oxygen (5%). The efficiency of DP cells reprogramming was ~0.03%, while the efficiency of dermal fibroblast reprogramming under the same conditions was ~0.01%. Therefore, we demonstrated the suitability of DP cells as an alternative source of iPS cells. A.I. Gordeyev 2014 /pmc/articles/PMC3999465/ /pubmed/24772326 Text en Copyright ® 2014 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Muchkaeva, I. A.
Dashinimaev, E. B.
Artyuhov, A. S.
Myagkova, E. P.
Vorotelyak, E. A.
Yegorov, Y. Y.
Vishnyakova, K. S.
Kravchenko, I. E.
Chumakov, P. M.
Terskikh, V. V.
Vasiliev, A. V.
Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells
title Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells
title_full Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells
title_fullStr Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells
title_full_unstemmed Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells
title_short Generation of iPS Cells from Human Hair Follice Dermal Papilla Cells
title_sort generation of ips cells from human hair follice dermal papilla cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999465/
https://www.ncbi.nlm.nih.gov/pubmed/24772326
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