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Extracellular biosynthesis of gadolinium oxide (Gd(2)O(3)) nanoparticles, their biodistribution and bioconjugation with the chemically modified anticancer drug taxol

As a part of our programme to develop nanobioconjugates for the treatment of cancer, we first synthesized extracellular, protein-capped, highly stable and well-dispersed gadolinium oxide (Gd(2)O(3)) nanoparticles by using thermophilic fungus Humicola sp. The biodistribution of the nanoparticles in r...

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Autores principales: Khan, Shadab Ali, Gambhir, Sanjay, Ahmad, Absar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Beilstein-Institut 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999844/
https://www.ncbi.nlm.nih.gov/pubmed/24778946
http://dx.doi.org/10.3762/bjnano.5.27
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author Khan, Shadab Ali
Gambhir, Sanjay
Ahmad, Absar
author_facet Khan, Shadab Ali
Gambhir, Sanjay
Ahmad, Absar
author_sort Khan, Shadab Ali
collection PubMed
description As a part of our programme to develop nanobioconjugates for the treatment of cancer, we first synthesized extracellular, protein-capped, highly stable and well-dispersed gadolinium oxide (Gd(2)O(3)) nanoparticles by using thermophilic fungus Humicola sp. The biodistribution of the nanoparticles in rats was checked by radiolabelling with Tc-99m. Finally, these nanoparticles were bioconjugated with the chemically modified anticancer drug taxol with the aim of characterizing the role of this bioconjugate in the treatment of cancer. The biosynthesized Gd(2)O(3) nanoparticles were characterized by UV–vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) and X-ray photoemission spectroscopy (XPS). The Gd(2)O(3)–taxol bioconjugate was confirmed by UV–vis spectroscopy and fluorescence microscopy and was purified by using high performance liquid chromatography (HPLC).
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spelling pubmed-39998442014-04-28 Extracellular biosynthesis of gadolinium oxide (Gd(2)O(3)) nanoparticles, their biodistribution and bioconjugation with the chemically modified anticancer drug taxol Khan, Shadab Ali Gambhir, Sanjay Ahmad, Absar Beilstein J Nanotechnol Full Research Paper As a part of our programme to develop nanobioconjugates for the treatment of cancer, we first synthesized extracellular, protein-capped, highly stable and well-dispersed gadolinium oxide (Gd(2)O(3)) nanoparticles by using thermophilic fungus Humicola sp. The biodistribution of the nanoparticles in rats was checked by radiolabelling with Tc-99m. Finally, these nanoparticles were bioconjugated with the chemically modified anticancer drug taxol with the aim of characterizing the role of this bioconjugate in the treatment of cancer. The biosynthesized Gd(2)O(3) nanoparticles were characterized by UV–vis spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD) and X-ray photoemission spectroscopy (XPS). The Gd(2)O(3)–taxol bioconjugate was confirmed by UV–vis spectroscopy and fluorescence microscopy and was purified by using high performance liquid chromatography (HPLC). Beilstein-Institut 2014-03-07 /pmc/articles/PMC3999844/ /pubmed/24778946 http://dx.doi.org/10.3762/bjnano.5.27 Text en Copyright © 2014, Khan et al. https://creativecommons.org/licenses/by/2.0https://www.beilstein-journals.org/bjnano/termsThis is an Open Access article under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The license is subject to the Beilstein Journal of Nanotechnology terms and conditions: (https://www.beilstein-journals.org/bjnano/terms)
spellingShingle Full Research Paper
Khan, Shadab Ali
Gambhir, Sanjay
Ahmad, Absar
Extracellular biosynthesis of gadolinium oxide (Gd(2)O(3)) nanoparticles, their biodistribution and bioconjugation with the chemically modified anticancer drug taxol
title Extracellular biosynthesis of gadolinium oxide (Gd(2)O(3)) nanoparticles, their biodistribution and bioconjugation with the chemically modified anticancer drug taxol
title_full Extracellular biosynthesis of gadolinium oxide (Gd(2)O(3)) nanoparticles, their biodistribution and bioconjugation with the chemically modified anticancer drug taxol
title_fullStr Extracellular biosynthesis of gadolinium oxide (Gd(2)O(3)) nanoparticles, their biodistribution and bioconjugation with the chemically modified anticancer drug taxol
title_full_unstemmed Extracellular biosynthesis of gadolinium oxide (Gd(2)O(3)) nanoparticles, their biodistribution and bioconjugation with the chemically modified anticancer drug taxol
title_short Extracellular biosynthesis of gadolinium oxide (Gd(2)O(3)) nanoparticles, their biodistribution and bioconjugation with the chemically modified anticancer drug taxol
title_sort extracellular biosynthesis of gadolinium oxide (gd(2)o(3)) nanoparticles, their biodistribution and bioconjugation with the chemically modified anticancer drug taxol
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3999844/
https://www.ncbi.nlm.nih.gov/pubmed/24778946
http://dx.doi.org/10.3762/bjnano.5.27
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