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Numb Promotes Cell Proliferation and Correlates with Poor Prognosis in Hepatocellular Carcinoma
BACKGROUND: Numb is an evolutionary conserved protein that plays critical roles in cell fate determination, cell adhesion, cell migration and a number of signaling pathways, but evidence for a substantial involvement of Numb in HCC has remained unclear. The present study was aimed to investigate the...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4000222/ https://www.ncbi.nlm.nih.gov/pubmed/24770339 http://dx.doi.org/10.1371/journal.pone.0095849 |
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author | Wu, Jian Shen, Shun-Li Chen, Bin Nie, Jing Peng, Bao-Gang |
author_facet | Wu, Jian Shen, Shun-Li Chen, Bin Nie, Jing Peng, Bao-Gang |
author_sort | Wu, Jian |
collection | PubMed |
description | BACKGROUND: Numb is an evolutionary conserved protein that plays critical roles in cell fate determination, cell adhesion, cell migration and a number of signaling pathways, but evidence for a substantial involvement of Numb in HCC has remained unclear. The present study was aimed to investigate the clinical and prognostic significance of Numb and its role in hepatocellular carcinoma (HCC). METHODOLOGY: The expression of Numb was detected in 107 cases of clinical paraffin-embedded hepatocellular carcinoma tissues,5 matched paris of fresh tissues and six hepatocellular cell lines by immunohistochemistry with clinicopathological analyses,RT-PCR or Western blot. Moreover, loss of function and gain of function assays were performed to evaluate the effect of Numb on cell proliferation in vitro. CONCLUSIONS: We found that Numb was obviously up-regulated in HCC tissues and cell lines (p<0.05). The Numb up-regulation correlated significantly with poor prognosis, and Numb status was identified as an independent prognostic factor. Over-expression of Numb increased proliferation in SMMC-7721 and BEL-7402 cells, while knock-down of Numb showed the opposite effect. Our study indicates that Numb up-regulation significantly correlates with cell proliferation and poor prognosis in hepatocellular carcinoma patients. It may be a useful biomarker for therapeutic strategy in hepatocellular carcinoma treatment. |
format | Online Article Text |
id | pubmed-4000222 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-40002222014-04-29 Numb Promotes Cell Proliferation and Correlates with Poor Prognosis in Hepatocellular Carcinoma Wu, Jian Shen, Shun-Li Chen, Bin Nie, Jing Peng, Bao-Gang PLoS One Research Article BACKGROUND: Numb is an evolutionary conserved protein that plays critical roles in cell fate determination, cell adhesion, cell migration and a number of signaling pathways, but evidence for a substantial involvement of Numb in HCC has remained unclear. The present study was aimed to investigate the clinical and prognostic significance of Numb and its role in hepatocellular carcinoma (HCC). METHODOLOGY: The expression of Numb was detected in 107 cases of clinical paraffin-embedded hepatocellular carcinoma tissues,5 matched paris of fresh tissues and six hepatocellular cell lines by immunohistochemistry with clinicopathological analyses,RT-PCR or Western blot. Moreover, loss of function and gain of function assays were performed to evaluate the effect of Numb on cell proliferation in vitro. CONCLUSIONS: We found that Numb was obviously up-regulated in HCC tissues and cell lines (p<0.05). The Numb up-regulation correlated significantly with poor prognosis, and Numb status was identified as an independent prognostic factor. Over-expression of Numb increased proliferation in SMMC-7721 and BEL-7402 cells, while knock-down of Numb showed the opposite effect. Our study indicates that Numb up-regulation significantly correlates with cell proliferation and poor prognosis in hepatocellular carcinoma patients. It may be a useful biomarker for therapeutic strategy in hepatocellular carcinoma treatment. Public Library of Science 2014-04-25 /pmc/articles/PMC4000222/ /pubmed/24770339 http://dx.doi.org/10.1371/journal.pone.0095849 Text en © 2014 Wu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Wu, Jian Shen, Shun-Li Chen, Bin Nie, Jing Peng, Bao-Gang Numb Promotes Cell Proliferation and Correlates with Poor Prognosis in Hepatocellular Carcinoma |
title | Numb Promotes Cell Proliferation and Correlates with Poor Prognosis in Hepatocellular Carcinoma |
title_full | Numb Promotes Cell Proliferation and Correlates with Poor Prognosis in Hepatocellular Carcinoma |
title_fullStr | Numb Promotes Cell Proliferation and Correlates with Poor Prognosis in Hepatocellular Carcinoma |
title_full_unstemmed | Numb Promotes Cell Proliferation and Correlates with Poor Prognosis in Hepatocellular Carcinoma |
title_short | Numb Promotes Cell Proliferation and Correlates with Poor Prognosis in Hepatocellular Carcinoma |
title_sort | numb promotes cell proliferation and correlates with poor prognosis in hepatocellular carcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4000222/ https://www.ncbi.nlm.nih.gov/pubmed/24770339 http://dx.doi.org/10.1371/journal.pone.0095849 |
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