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LIN28 Expression in Rat Spinal Cord After Injury

LIN28, an RNA-binding protein, is known to be involved in the regulation of many cellular processes, such as embryonic stem cell proliferation, cell fate succession, developmental timing, and oncogenesis. However, its expression and function in central nervous system still unclear. In this study, we...

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Autores principales: Yue, Ying, Zhang, Dongmei, Jiang, Shengyang, Li, Aihong, Guo, Aisong, Wu, Xinming, Xia, Xiaopeng, Cheng, Hongbing, Tao, Tao, Gu, Xingxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4000414/
https://www.ncbi.nlm.nih.gov/pubmed/24700281
http://dx.doi.org/10.1007/s11064-014-1278-2
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author Yue, Ying
Zhang, Dongmei
Jiang, Shengyang
Li, Aihong
Guo, Aisong
Wu, Xinming
Xia, Xiaopeng
Cheng, Hongbing
Tao, Tao
Gu, Xingxing
author_facet Yue, Ying
Zhang, Dongmei
Jiang, Shengyang
Li, Aihong
Guo, Aisong
Wu, Xinming
Xia, Xiaopeng
Cheng, Hongbing
Tao, Tao
Gu, Xingxing
author_sort Yue, Ying
collection PubMed
description LIN28, an RNA-binding protein, is known to be involved in the regulation of many cellular processes, such as embryonic stem cell proliferation, cell fate succession, developmental timing, and oncogenesis. However, its expression and function in central nervous system still unclear. In this study, we performed an acute spinal cord contusion injury (SCI) model in adult rats and investigated the dynamic changes of LIN28 expression in spinal cord. Western blot and immunohistochemistry analysis revealed that LIN28 was present in normal spinal cord. It gradually increased, reached a peak at 3 day, and then nearly declined to the basal level at 14 days after SCI. Double immunofluorescence staining showed that LIN28 immunoreactivity was found in neurons, astrocytes and a handful of microglia. Interestingly, LIN28 expression was increased predominantly in astrocytes but not in neurons. Moreover, the colocalization of LIN28 and proliferating cell nuclear antigen was detected after injury. Western blot showed that LIN28 participated in lipopolysaccharide (LPS) induced astrocytes inflammatory responses by NF-κB signaling pathway. These results suggested that LIN28 may be involved in the pathologic process of SCI, and further research is needed to have a good understanding of its function and mechanism.
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spelling pubmed-40004142014-05-07 LIN28 Expression in Rat Spinal Cord After Injury Yue, Ying Zhang, Dongmei Jiang, Shengyang Li, Aihong Guo, Aisong Wu, Xinming Xia, Xiaopeng Cheng, Hongbing Tao, Tao Gu, Xingxing Neurochem Res Original Paper LIN28, an RNA-binding protein, is known to be involved in the regulation of many cellular processes, such as embryonic stem cell proliferation, cell fate succession, developmental timing, and oncogenesis. However, its expression and function in central nervous system still unclear. In this study, we performed an acute spinal cord contusion injury (SCI) model in adult rats and investigated the dynamic changes of LIN28 expression in spinal cord. Western blot and immunohistochemistry analysis revealed that LIN28 was present in normal spinal cord. It gradually increased, reached a peak at 3 day, and then nearly declined to the basal level at 14 days after SCI. Double immunofluorescence staining showed that LIN28 immunoreactivity was found in neurons, astrocytes and a handful of microglia. Interestingly, LIN28 expression was increased predominantly in astrocytes but not in neurons. Moreover, the colocalization of LIN28 and proliferating cell nuclear antigen was detected after injury. Western blot showed that LIN28 participated in lipopolysaccharide (LPS) induced astrocytes inflammatory responses by NF-κB signaling pathway. These results suggested that LIN28 may be involved in the pathologic process of SCI, and further research is needed to have a good understanding of its function and mechanism. Springer US 2014-04-04 2014 /pmc/articles/PMC4000414/ /pubmed/24700281 http://dx.doi.org/10.1007/s11064-014-1278-2 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Paper
Yue, Ying
Zhang, Dongmei
Jiang, Shengyang
Li, Aihong
Guo, Aisong
Wu, Xinming
Xia, Xiaopeng
Cheng, Hongbing
Tao, Tao
Gu, Xingxing
LIN28 Expression in Rat Spinal Cord After Injury
title LIN28 Expression in Rat Spinal Cord After Injury
title_full LIN28 Expression in Rat Spinal Cord After Injury
title_fullStr LIN28 Expression in Rat Spinal Cord After Injury
title_full_unstemmed LIN28 Expression in Rat Spinal Cord After Injury
title_short LIN28 Expression in Rat Spinal Cord After Injury
title_sort lin28 expression in rat spinal cord after injury
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4000414/
https://www.ncbi.nlm.nih.gov/pubmed/24700281
http://dx.doi.org/10.1007/s11064-014-1278-2
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