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LIN28 Expression in Rat Spinal Cord After Injury
LIN28, an RNA-binding protein, is known to be involved in the regulation of many cellular processes, such as embryonic stem cell proliferation, cell fate succession, developmental timing, and oncogenesis. However, its expression and function in central nervous system still unclear. In this study, we...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4000414/ https://www.ncbi.nlm.nih.gov/pubmed/24700281 http://dx.doi.org/10.1007/s11064-014-1278-2 |
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author | Yue, Ying Zhang, Dongmei Jiang, Shengyang Li, Aihong Guo, Aisong Wu, Xinming Xia, Xiaopeng Cheng, Hongbing Tao, Tao Gu, Xingxing |
author_facet | Yue, Ying Zhang, Dongmei Jiang, Shengyang Li, Aihong Guo, Aisong Wu, Xinming Xia, Xiaopeng Cheng, Hongbing Tao, Tao Gu, Xingxing |
author_sort | Yue, Ying |
collection | PubMed |
description | LIN28, an RNA-binding protein, is known to be involved in the regulation of many cellular processes, such as embryonic stem cell proliferation, cell fate succession, developmental timing, and oncogenesis. However, its expression and function in central nervous system still unclear. In this study, we performed an acute spinal cord contusion injury (SCI) model in adult rats and investigated the dynamic changes of LIN28 expression in spinal cord. Western blot and immunohistochemistry analysis revealed that LIN28 was present in normal spinal cord. It gradually increased, reached a peak at 3 day, and then nearly declined to the basal level at 14 days after SCI. Double immunofluorescence staining showed that LIN28 immunoreactivity was found in neurons, astrocytes and a handful of microglia. Interestingly, LIN28 expression was increased predominantly in astrocytes but not in neurons. Moreover, the colocalization of LIN28 and proliferating cell nuclear antigen was detected after injury. Western blot showed that LIN28 participated in lipopolysaccharide (LPS) induced astrocytes inflammatory responses by NF-κB signaling pathway. These results suggested that LIN28 may be involved in the pathologic process of SCI, and further research is needed to have a good understanding of its function and mechanism. |
format | Online Article Text |
id | pubmed-4000414 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-40004142014-05-07 LIN28 Expression in Rat Spinal Cord After Injury Yue, Ying Zhang, Dongmei Jiang, Shengyang Li, Aihong Guo, Aisong Wu, Xinming Xia, Xiaopeng Cheng, Hongbing Tao, Tao Gu, Xingxing Neurochem Res Original Paper LIN28, an RNA-binding protein, is known to be involved in the regulation of many cellular processes, such as embryonic stem cell proliferation, cell fate succession, developmental timing, and oncogenesis. However, its expression and function in central nervous system still unclear. In this study, we performed an acute spinal cord contusion injury (SCI) model in adult rats and investigated the dynamic changes of LIN28 expression in spinal cord. Western blot and immunohistochemistry analysis revealed that LIN28 was present in normal spinal cord. It gradually increased, reached a peak at 3 day, and then nearly declined to the basal level at 14 days after SCI. Double immunofluorescence staining showed that LIN28 immunoreactivity was found in neurons, astrocytes and a handful of microglia. Interestingly, LIN28 expression was increased predominantly in astrocytes but not in neurons. Moreover, the colocalization of LIN28 and proliferating cell nuclear antigen was detected after injury. Western blot showed that LIN28 participated in lipopolysaccharide (LPS) induced astrocytes inflammatory responses by NF-κB signaling pathway. These results suggested that LIN28 may be involved in the pathologic process of SCI, and further research is needed to have a good understanding of its function and mechanism. Springer US 2014-04-04 2014 /pmc/articles/PMC4000414/ /pubmed/24700281 http://dx.doi.org/10.1007/s11064-014-1278-2 Text en © The Author(s) 2014 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Original Paper Yue, Ying Zhang, Dongmei Jiang, Shengyang Li, Aihong Guo, Aisong Wu, Xinming Xia, Xiaopeng Cheng, Hongbing Tao, Tao Gu, Xingxing LIN28 Expression in Rat Spinal Cord After Injury |
title | LIN28 Expression in Rat Spinal Cord After Injury |
title_full | LIN28 Expression in Rat Spinal Cord After Injury |
title_fullStr | LIN28 Expression in Rat Spinal Cord After Injury |
title_full_unstemmed | LIN28 Expression in Rat Spinal Cord After Injury |
title_short | LIN28 Expression in Rat Spinal Cord After Injury |
title_sort | lin28 expression in rat spinal cord after injury |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4000414/ https://www.ncbi.nlm.nih.gov/pubmed/24700281 http://dx.doi.org/10.1007/s11064-014-1278-2 |
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