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Asymmetric localization of natural antisense RNA of neuropeptide sensorin in Aplysia sensory neurons during aging and activity

Despite the advances in our understanding of transcriptome, regulation and function of its non-coding components continue to be poorly understood. Here we searched for natural antisense transcript for sensorin (NAT-SRN), a neuropeptide expressed in the presynaptic sensory neurons of gill-withdrawal...

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Autores principales: Kadakkuzha, Beena M., Liu, Xin-An, Narvaez, Maria, Kaye, Alexandra, Akhmedov, Komolitdin, Puthanveettil, Sathyanarayanan V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4001032/
https://www.ncbi.nlm.nih.gov/pubmed/24795747
http://dx.doi.org/10.3389/fgene.2014.00084
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author Kadakkuzha, Beena M.
Liu, Xin-An
Narvaez, Maria
Kaye, Alexandra
Akhmedov, Komolitdin
Puthanveettil, Sathyanarayanan V.
author_facet Kadakkuzha, Beena M.
Liu, Xin-An
Narvaez, Maria
Kaye, Alexandra
Akhmedov, Komolitdin
Puthanveettil, Sathyanarayanan V.
author_sort Kadakkuzha, Beena M.
collection PubMed
description Despite the advances in our understanding of transcriptome, regulation and function of its non-coding components continue to be poorly understood. Here we searched for natural antisense transcript for sensorin (NAT-SRN), a neuropeptide expressed in the presynaptic sensory neurons of gill-withdrawal reflex of the marine snail Aplysia californica. Sensorin (SRN) has a key role in learning and long-term memory storage in Aplysia. We have now identified NAT-SRN in the central nervous system (CNS) and have confirmed its expression by northern blotting and fluorescent RNA in situ hybridization. Quantitative analysis of NAT-SRN in micro-dissected cell bodies and processes of sensory neurons suggest that NAT-SRN is present in the distal neuronal processes along with sense transcripts. Importantly, aging is associated with reduction in levels of NAT-SRN in sensory neuron processes. Furthermore, we find that forskolin, an activator of CREB signaling, differentially alters the distribution of SRN and NAT-SRN. These studies reveal novel insights into physiological regulation of natural antisense RNAs.
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spelling pubmed-40010322014-05-02 Asymmetric localization of natural antisense RNA of neuropeptide sensorin in Aplysia sensory neurons during aging and activity Kadakkuzha, Beena M. Liu, Xin-An Narvaez, Maria Kaye, Alexandra Akhmedov, Komolitdin Puthanveettil, Sathyanarayanan V. Front Genet Genetics Despite the advances in our understanding of transcriptome, regulation and function of its non-coding components continue to be poorly understood. Here we searched for natural antisense transcript for sensorin (NAT-SRN), a neuropeptide expressed in the presynaptic sensory neurons of gill-withdrawal reflex of the marine snail Aplysia californica. Sensorin (SRN) has a key role in learning and long-term memory storage in Aplysia. We have now identified NAT-SRN in the central nervous system (CNS) and have confirmed its expression by northern blotting and fluorescent RNA in situ hybridization. Quantitative analysis of NAT-SRN in micro-dissected cell bodies and processes of sensory neurons suggest that NAT-SRN is present in the distal neuronal processes along with sense transcripts. Importantly, aging is associated with reduction in levels of NAT-SRN in sensory neuron processes. Furthermore, we find that forskolin, an activator of CREB signaling, differentially alters the distribution of SRN and NAT-SRN. These studies reveal novel insights into physiological regulation of natural antisense RNAs. Frontiers Media S.A. 2014-04-22 /pmc/articles/PMC4001032/ /pubmed/24795747 http://dx.doi.org/10.3389/fgene.2014.00084 Text en Copyright © 2014 Kadakkuzha, Liu, Narvaez, Kaye, Akhmedov and Puthanveettil. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Kadakkuzha, Beena M.
Liu, Xin-An
Narvaez, Maria
Kaye, Alexandra
Akhmedov, Komolitdin
Puthanveettil, Sathyanarayanan V.
Asymmetric localization of natural antisense RNA of neuropeptide sensorin in Aplysia sensory neurons during aging and activity
title Asymmetric localization of natural antisense RNA of neuropeptide sensorin in Aplysia sensory neurons during aging and activity
title_full Asymmetric localization of natural antisense RNA of neuropeptide sensorin in Aplysia sensory neurons during aging and activity
title_fullStr Asymmetric localization of natural antisense RNA of neuropeptide sensorin in Aplysia sensory neurons during aging and activity
title_full_unstemmed Asymmetric localization of natural antisense RNA of neuropeptide sensorin in Aplysia sensory neurons during aging and activity
title_short Asymmetric localization of natural antisense RNA of neuropeptide sensorin in Aplysia sensory neurons during aging and activity
title_sort asymmetric localization of natural antisense rna of neuropeptide sensorin in aplysia sensory neurons during aging and activity
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4001032/
https://www.ncbi.nlm.nih.gov/pubmed/24795747
http://dx.doi.org/10.3389/fgene.2014.00084
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