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The effect of inhibition of host MreB on the infection of thermophilic phage GVE2 in high temperature environment

In eukaryotes, the manipulation of the host actin cytoskeleton is a necessary strategy for viral pathogens to invade host cells. Increasing evidence indicates that the actin homolog MreB of bacteria plays key roles in cell shape formation, cell polarity, cell wall biosynthesis, and chromosome segreg...

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Detalles Bibliográficos
Autores principales: Jin, Min, Chen, Yanjiang, Xu, Chenxi, Zhang, Xiaobo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4001104/
https://www.ncbi.nlm.nih.gov/pubmed/24769758
http://dx.doi.org/10.1038/srep04823
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author Jin, Min
Chen, Yanjiang
Xu, Chenxi
Zhang, Xiaobo
author_facet Jin, Min
Chen, Yanjiang
Xu, Chenxi
Zhang, Xiaobo
author_sort Jin, Min
collection PubMed
description In eukaryotes, the manipulation of the host actin cytoskeleton is a necessary strategy for viral pathogens to invade host cells. Increasing evidence indicates that the actin homolog MreB of bacteria plays key roles in cell shape formation, cell polarity, cell wall biosynthesis, and chromosome segregation. However, the role of bacterial MreB in the bacteriophage infection is not extensively investigated. To address this issue, in this study, the MreB of thermophilic Geobacillus sp. E263 from a deep-sea hydrothermal field was characterized by inhibiting the MreB polymerization and subsequently evaluating the bacteriophage GVE2 infection. The results showed that the host MreB played important roles in the bacteriophage infection at high temperature. After the host cells were treated with small molecule drug A22 or MP265, the specific inhibitors of MreB polymerization, the adsorption of GVE2 and the replication of GVE2 genome were significantly repressed. The confocal microscopy data revealed that MreB facilitated the GVE2 infection by inducing the polar distribution of virions during the phage infection. Our study contributed novel information to understand the molecular events of the host in response to bacteriophage challenge and extended our knowledge about the host-virus interaction in deep-sea vent ecosystems.
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spelling pubmed-40011042014-04-28 The effect of inhibition of host MreB on the infection of thermophilic phage GVE2 in high temperature environment Jin, Min Chen, Yanjiang Xu, Chenxi Zhang, Xiaobo Sci Rep Article In eukaryotes, the manipulation of the host actin cytoskeleton is a necessary strategy for viral pathogens to invade host cells. Increasing evidence indicates that the actin homolog MreB of bacteria plays key roles in cell shape formation, cell polarity, cell wall biosynthesis, and chromosome segregation. However, the role of bacterial MreB in the bacteriophage infection is not extensively investigated. To address this issue, in this study, the MreB of thermophilic Geobacillus sp. E263 from a deep-sea hydrothermal field was characterized by inhibiting the MreB polymerization and subsequently evaluating the bacteriophage GVE2 infection. The results showed that the host MreB played important roles in the bacteriophage infection at high temperature. After the host cells were treated with small molecule drug A22 or MP265, the specific inhibitors of MreB polymerization, the adsorption of GVE2 and the replication of GVE2 genome were significantly repressed. The confocal microscopy data revealed that MreB facilitated the GVE2 infection by inducing the polar distribution of virions during the phage infection. Our study contributed novel information to understand the molecular events of the host in response to bacteriophage challenge and extended our knowledge about the host-virus interaction in deep-sea vent ecosystems. Nature Publishing Group 2014-04-28 /pmc/articles/PMC4001104/ /pubmed/24769758 http://dx.doi.org/10.1038/srep04823 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images in this article are included in the article's Creative Commons license, unless indicated otherwise in the image credit; if the image is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the image. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Article
Jin, Min
Chen, Yanjiang
Xu, Chenxi
Zhang, Xiaobo
The effect of inhibition of host MreB on the infection of thermophilic phage GVE2 in high temperature environment
title The effect of inhibition of host MreB on the infection of thermophilic phage GVE2 in high temperature environment
title_full The effect of inhibition of host MreB on the infection of thermophilic phage GVE2 in high temperature environment
title_fullStr The effect of inhibition of host MreB on the infection of thermophilic phage GVE2 in high temperature environment
title_full_unstemmed The effect of inhibition of host MreB on the infection of thermophilic phage GVE2 in high temperature environment
title_short The effect of inhibition of host MreB on the infection of thermophilic phage GVE2 in high temperature environment
title_sort effect of inhibition of host mreb on the infection of thermophilic phage gve2 in high temperature environment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4001104/
https://www.ncbi.nlm.nih.gov/pubmed/24769758
http://dx.doi.org/10.1038/srep04823
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