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Thoracic epidural anesthesia decreases endotoxin-induced endothelial injury

BACKGROUND: The sympathetic nervous system is considered to modulate the endotoxin-induced activation of immune cells. Here we investigate whether thoracic epidural anesthesia with its regional symapathetic blocking effect alters endotoxin-induced leukocyte-endothelium activation and interaction wit...

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Autores principales: Enigk, Fabian, Wagner, Antje, Samapati, Rudi, Rittner, Heike, Brack, Alexander, Mousa, Shaaban A, Schäfer, Michael, Habazettl, Helmut, Schäper, Jörn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4001105/
https://www.ncbi.nlm.nih.gov/pubmed/24708631
http://dx.doi.org/10.1186/1471-2253-14-23
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author Enigk, Fabian
Wagner, Antje
Samapati, Rudi
Rittner, Heike
Brack, Alexander
Mousa, Shaaban A
Schäfer, Michael
Habazettl, Helmut
Schäper, Jörn
author_facet Enigk, Fabian
Wagner, Antje
Samapati, Rudi
Rittner, Heike
Brack, Alexander
Mousa, Shaaban A
Schäfer, Michael
Habazettl, Helmut
Schäper, Jörn
author_sort Enigk, Fabian
collection PubMed
description BACKGROUND: The sympathetic nervous system is considered to modulate the endotoxin-induced activation of immune cells. Here we investigate whether thoracic epidural anesthesia with its regional symapathetic blocking effect alters endotoxin-induced leukocyte-endothelium activation and interaction with subsequent endothelial injury. METHODS: Sprague Dawley rats were anesthetized, cannulated and hemodynamically monitored. E. coli lipopolysaccharide (Serotype 0127:B8, 1.5 mg x kg(-1) x h(-1)) or isotonic saline (controls) was infused for 300 minutes. An epidural catheter was inserted for continuous application of lidocaine or normal saline in endotoxemic animals and saline in controls. After 300 minutes we measured catecholamine and cytokine plasma concentrations, adhesion molecule expression, leukocyte adhesion, and intestinal tissue edema. RESULTS: In endotoxemic animals with epidural saline, LPS significantly increased the interleukin-1β plasma concentration (48%), the expression of endothelial adhesion molecules E-selectin (34%) and ICAM-1 (42%), and the number of adherent leukocytes (40%) with an increase in intestinal myeloperoxidase activity (26%) and tissue edema (75%) when compared to healthy controls. In endotoxemic animals with epidural infusion of lidocaine the values were similar to those in control animals, while epinephrine plasma concentration was 32% lower compared to endotoxemic animals with epidural saline. CONCLUSIONS: Thoracic epidural anesthesia attenuated the endotoxin-induced increase of IL-1β concentration, adhesion molecule expression and leukocyte-adhesion with subsequent endothelial injury. A potential mechanism is the reduction in the plasma concentration of epinephrine.
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spelling pubmed-40011052014-04-29 Thoracic epidural anesthesia decreases endotoxin-induced endothelial injury Enigk, Fabian Wagner, Antje Samapati, Rudi Rittner, Heike Brack, Alexander Mousa, Shaaban A Schäfer, Michael Habazettl, Helmut Schäper, Jörn BMC Anesthesiol Research Article BACKGROUND: The sympathetic nervous system is considered to modulate the endotoxin-induced activation of immune cells. Here we investigate whether thoracic epidural anesthesia with its regional symapathetic blocking effect alters endotoxin-induced leukocyte-endothelium activation and interaction with subsequent endothelial injury. METHODS: Sprague Dawley rats were anesthetized, cannulated and hemodynamically monitored. E. coli lipopolysaccharide (Serotype 0127:B8, 1.5 mg x kg(-1) x h(-1)) or isotonic saline (controls) was infused for 300 minutes. An epidural catheter was inserted for continuous application of lidocaine or normal saline in endotoxemic animals and saline in controls. After 300 minutes we measured catecholamine and cytokine plasma concentrations, adhesion molecule expression, leukocyte adhesion, and intestinal tissue edema. RESULTS: In endotoxemic animals with epidural saline, LPS significantly increased the interleukin-1β plasma concentration (48%), the expression of endothelial adhesion molecules E-selectin (34%) and ICAM-1 (42%), and the number of adherent leukocytes (40%) with an increase in intestinal myeloperoxidase activity (26%) and tissue edema (75%) when compared to healthy controls. In endotoxemic animals with epidural infusion of lidocaine the values were similar to those in control animals, while epinephrine plasma concentration was 32% lower compared to endotoxemic animals with epidural saline. CONCLUSIONS: Thoracic epidural anesthesia attenuated the endotoxin-induced increase of IL-1β concentration, adhesion molecule expression and leukocyte-adhesion with subsequent endothelial injury. A potential mechanism is the reduction in the plasma concentration of epinephrine. BioMed Central 2014-04-05 /pmc/articles/PMC4001105/ /pubmed/24708631 http://dx.doi.org/10.1186/1471-2253-14-23 Text en Copyright © 2014 Enigk et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Enigk, Fabian
Wagner, Antje
Samapati, Rudi
Rittner, Heike
Brack, Alexander
Mousa, Shaaban A
Schäfer, Michael
Habazettl, Helmut
Schäper, Jörn
Thoracic epidural anesthesia decreases endotoxin-induced endothelial injury
title Thoracic epidural anesthesia decreases endotoxin-induced endothelial injury
title_full Thoracic epidural anesthesia decreases endotoxin-induced endothelial injury
title_fullStr Thoracic epidural anesthesia decreases endotoxin-induced endothelial injury
title_full_unstemmed Thoracic epidural anesthesia decreases endotoxin-induced endothelial injury
title_short Thoracic epidural anesthesia decreases endotoxin-induced endothelial injury
title_sort thoracic epidural anesthesia decreases endotoxin-induced endothelial injury
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4001105/
https://www.ncbi.nlm.nih.gov/pubmed/24708631
http://dx.doi.org/10.1186/1471-2253-14-23
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