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Rapid Site-Directed Mutagenesis Using Two-PCR-Generated DNA Fragments Reproducing the Plasmid Template

We describe a new rapid and efficient polymerase chain reaction (PCR)-based site-directed mutagenesis method. This procedure is effective with any plasmid and it employs four oligonucleotide primers. One primer contains the desired mutation, the second is oriented in the opposite direction (one of t...

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Detalles Bibliográficos
Autores principales:	Allemandou, Flore, Nussberger, Jürg, Brunner, Hans R., Brakch, Noureddine
Formato:	Texto
Lenguaje:	English
Publicado:	Hindawi Publishing Corporation 2003
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC400210/ https://www.ncbi.nlm.nih.gov/pubmed/12975535 http://dx.doi.org/10.1155/S1110724303209141

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