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EPO‐receptor is present in mouse C2C12 and human primary skeletal muscle cells but EPO does not influence myogenesis

The role and regulation of the pleiotropic cytokine erythropoietin (EPO) in skeletal muscle are controversial. EPO exerts its effects by binding its specific receptor (EPO‐R), which activates intracellular signaling and gene transcription in response to internal and external stress signals. EPO is s...

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Detalles Bibliográficos
Autores principales: Lamon, Séverine, Zacharewicz, Evelyn, Stephens, Andrew N., Russell, Aaron P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wiley Periodicals, Inc. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4002236/
https://www.ncbi.nlm.nih.gov/pubmed/24760510
http://dx.doi.org/10.1002/phy2.256
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author Lamon, Séverine
Zacharewicz, Evelyn
Stephens, Andrew N.
Russell, Aaron P.
author_facet Lamon, Séverine
Zacharewicz, Evelyn
Stephens, Andrew N.
Russell, Aaron P.
author_sort Lamon, Séverine
collection PubMed
description The role and regulation of the pleiotropic cytokine erythropoietin (EPO) in skeletal muscle are controversial. EPO exerts its effects by binding its specific receptor (EPO‐R), which activates intracellular signaling and gene transcription in response to internal and external stress signals. EPO is suggested to play a direct role in myogenesis via the EPO‐R, but several studies have questioned the effect of EPO treatment in muscle in vitro and in vivo. The lack of certainty surrounding the use of nonspecific EPO‐R antibodies contributes to the ambiguity of the field. Our study demonstrates that the EPO‐R gene and protein are expressed at each stage of mouse C2C12 and human skeletal muscle cell proliferation and differentiation and validates a specific antibody for the detection of the EPO‐R protein. However, in our experimental conditions, EPO treatment had no effect on mouse C2C12 and human muscle cell proliferation, differentiation, protein synthesis or EPO‐R expression. While an increase in Akt and MAPK phosphorylation was observed, we demonstrate that this effect resulted from the stress caused by changing medium and not from EPO treatment. We therefore suggest that skeletal muscle EPO‐R might be present in a nonfunctional form, or too lowly expressed to play a role in muscle cell function.
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spelling pubmed-40022362014-05-13 EPO‐receptor is present in mouse C2C12 and human primary skeletal muscle cells but EPO does not influence myogenesis Lamon, Séverine Zacharewicz, Evelyn Stephens, Andrew N. Russell, Aaron P. Physiol Rep Original Research The role and regulation of the pleiotropic cytokine erythropoietin (EPO) in skeletal muscle are controversial. EPO exerts its effects by binding its specific receptor (EPO‐R), which activates intracellular signaling and gene transcription in response to internal and external stress signals. EPO is suggested to play a direct role in myogenesis via the EPO‐R, but several studies have questioned the effect of EPO treatment in muscle in vitro and in vivo. The lack of certainty surrounding the use of nonspecific EPO‐R antibodies contributes to the ambiguity of the field. Our study demonstrates that the EPO‐R gene and protein are expressed at each stage of mouse C2C12 and human skeletal muscle cell proliferation and differentiation and validates a specific antibody for the detection of the EPO‐R protein. However, in our experimental conditions, EPO treatment had no effect on mouse C2C12 and human muscle cell proliferation, differentiation, protein synthesis or EPO‐R expression. While an increase in Akt and MAPK phosphorylation was observed, we demonstrate that this effect resulted from the stress caused by changing medium and not from EPO treatment. We therefore suggest that skeletal muscle EPO‐R might be present in a nonfunctional form, or too lowly expressed to play a role in muscle cell function. Wiley Periodicals, Inc. 2014-03-26 /pmc/articles/PMC4002236/ /pubmed/24760510 http://dx.doi.org/10.1002/phy2.256 Text en © 2014 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. http://creativecommons.org/licenses/by/3.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Lamon, Séverine
Zacharewicz, Evelyn
Stephens, Andrew N.
Russell, Aaron P.
EPO‐receptor is present in mouse C2C12 and human primary skeletal muscle cells but EPO does not influence myogenesis
title EPO‐receptor is present in mouse C2C12 and human primary skeletal muscle cells but EPO does not influence myogenesis
title_full EPO‐receptor is present in mouse C2C12 and human primary skeletal muscle cells but EPO does not influence myogenesis
title_fullStr EPO‐receptor is present in mouse C2C12 and human primary skeletal muscle cells but EPO does not influence myogenesis
title_full_unstemmed EPO‐receptor is present in mouse C2C12 and human primary skeletal muscle cells but EPO does not influence myogenesis
title_short EPO‐receptor is present in mouse C2C12 and human primary skeletal muscle cells but EPO does not influence myogenesis
title_sort epo‐receptor is present in mouse c2c12 and human primary skeletal muscle cells but epo does not influence myogenesis
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4002236/
https://www.ncbi.nlm.nih.gov/pubmed/24760510
http://dx.doi.org/10.1002/phy2.256
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