Semi-quantitative analysis of transcript accumulation in response to drought stress by Lepidium latifolium seedlings

Cross-amplification of five Arabidopsis abiotic stress-responsive genes (AtPAP, ZFAN, Vn, LC4 and SNS) in Lepidium has been documented in plants raised out of seeds pre-treated with potassium nitrate (KNO(3)) for assessment of enhanced drought stress tolerance. cDNA was synthesized from Lepidium pla...

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Detalles Bibliográficos
Autores principales: Mohan Gupta, Sanjay, Singh, Sadhana, Pandey, Pankaj, Grover, Atul, Ahmed, Zakwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4002603/
https://www.ncbi.nlm.nih.gov/pubmed/23838956
http://dx.doi.org/10.4161/psb.25388
Descripción
Sumario:Cross-amplification of five Arabidopsis abiotic stress-responsive genes (AtPAP, ZFAN, Vn, LC4 and SNS) in Lepidium has been documented in plants raised out of seeds pre-treated with potassium nitrate (KNO(3)) for assessment of enhanced drought stress tolerance. cDNA was synthesized from Lepidium plants pre-treated with KNO(3) (0.1% and 0.3%) and exposed to drought conditions (5% and 15% PEG) at seedling stage for 30 d. Transcript accumulation of all the five genes were found suppressed in set of seedlings, which were pre-treated with 0.1% KNO(3) and were exposed to 15% PEG for 30 d. The present study establishes that different pre-treatments may further enhance the survivability of Lepidium plants under conditions of drought stress to different degrees.

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