Semi-quantitative analysis of transcript accumulation in response to drought stress by Lepidium latifolium seedlings

Cross-amplification of five Arabidopsis abiotic stress-responsive genes (AtPAP, ZFAN, Vn, LC4 and SNS) in Lepidium has been documented in plants raised out of seeds pre-treated with potassium nitrate (KNO(3)) for assessment of enhanced drought stress tolerance. cDNA was synthesized from Lepidium pla...

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Autores principales: Mohan Gupta, Sanjay, Singh, Sadhana, Pandey, Pankaj, Grover, Atul, Ahmed, Zakwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4002603/
https://www.ncbi.nlm.nih.gov/pubmed/23838956
http://dx.doi.org/10.4161/psb.25388
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author Mohan Gupta, Sanjay
Singh, Sadhana
Pandey, Pankaj
Grover, Atul
Ahmed, Zakwan
author_facet Mohan Gupta, Sanjay
Singh, Sadhana
Pandey, Pankaj
Grover, Atul
Ahmed, Zakwan
author_sort Mohan Gupta, Sanjay
collection PubMed
description Cross-amplification of five Arabidopsis abiotic stress-responsive genes (AtPAP, ZFAN, Vn, LC4 and SNS) in Lepidium has been documented in plants raised out of seeds pre-treated with potassium nitrate (KNO(3)) for assessment of enhanced drought stress tolerance. cDNA was synthesized from Lepidium plants pre-treated with KNO(3) (0.1% and 0.3%) and exposed to drought conditions (5% and 15% PEG) at seedling stage for 30 d. Transcript accumulation of all the five genes were found suppressed in set of seedlings, which were pre-treated with 0.1% KNO(3) and were exposed to 15% PEG for 30 d. The present study establishes that different pre-treatments may further enhance the survivability of Lepidium plants under conditions of drought stress to different degrees.
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spelling pubmed-40026032014-04-30 Semi-quantitative analysis of transcript accumulation in response to drought stress by Lepidium latifolium seedlings Mohan Gupta, Sanjay Singh, Sadhana Pandey, Pankaj Grover, Atul Ahmed, Zakwan Plant Signal Behav Research Paper Cross-amplification of five Arabidopsis abiotic stress-responsive genes (AtPAP, ZFAN, Vn, LC4 and SNS) in Lepidium has been documented in plants raised out of seeds pre-treated with potassium nitrate (KNO(3)) for assessment of enhanced drought stress tolerance. cDNA was synthesized from Lepidium plants pre-treated with KNO(3) (0.1% and 0.3%) and exposed to drought conditions (5% and 15% PEG) at seedling stage for 30 d. Transcript accumulation of all the five genes were found suppressed in set of seedlings, which were pre-treated with 0.1% KNO(3) and were exposed to 15% PEG for 30 d. The present study establishes that different pre-treatments may further enhance the survivability of Lepidium plants under conditions of drought stress to different degrees. Landes Bioscience 2013-09-01 2013-06-24 /pmc/articles/PMC4002603/ /pubmed/23838956 http://dx.doi.org/10.4161/psb.25388 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Research Paper
Mohan Gupta, Sanjay
Singh, Sadhana
Pandey, Pankaj
Grover, Atul
Ahmed, Zakwan
Semi-quantitative analysis of transcript accumulation in response to drought stress by Lepidium latifolium seedlings
title Semi-quantitative analysis of transcript accumulation in response to drought stress by Lepidium latifolium seedlings
title_full Semi-quantitative analysis of transcript accumulation in response to drought stress by Lepidium latifolium seedlings
title_fullStr Semi-quantitative analysis of transcript accumulation in response to drought stress by Lepidium latifolium seedlings
title_full_unstemmed Semi-quantitative analysis of transcript accumulation in response to drought stress by Lepidium latifolium seedlings
title_short Semi-quantitative analysis of transcript accumulation in response to drought stress by Lepidium latifolium seedlings
title_sort semi-quantitative analysis of transcript accumulation in response to drought stress by lepidium latifolium seedlings
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4002603/
https://www.ncbi.nlm.nih.gov/pubmed/23838956
http://dx.doi.org/10.4161/psb.25388
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