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Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa

Several studies exhibit the presence of Ricinus Communis Agglutinin I (RCA) binding glycocalyx in mammalian spermatozoa. However, the molecular characterization of RCA binding glycocalyx in sperm membranes and its mechanism of action are poorly understood. The objective of the study was to identify...

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Autores principales: Nagdas, Subir K., McLean, Eric L., Richardson, Leeá P., Raychoudhury, Samir
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4003772/
https://www.ncbi.nlm.nih.gov/pubmed/24818024
http://dx.doi.org/10.1155/2014/573293
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author Nagdas, Subir K.
McLean, Eric L.
Richardson, Leeá P.
Raychoudhury, Samir
author_facet Nagdas, Subir K.
McLean, Eric L.
Richardson, Leeá P.
Raychoudhury, Samir
author_sort Nagdas, Subir K.
collection PubMed
description Several studies exhibit the presence of Ricinus Communis Agglutinin I (RCA) binding glycocalyx in mammalian spermatozoa. However, the molecular characterization of RCA binding glycocalyx in sperm membranes and its mechanism of action are poorly understood. The objective of the study was to identify and to characterize RCA binding glycoprotein of the bovine sperm plasma membranes (PM). Lectin blots of caput and cauda sperm PM revealed a 38 kDa polypeptide exhibiting the highest affinity to RCA among the several major RCA binding polypeptides. The 38 kDa RCA binding polypeptide of cauda sperm PM was purified and exhibited a charge train of three distinct spots with isoelectric points (pH 5.3 and 5.8). Proteomic identification yielded ten peptides that matched the sequence of Testis Expressed 101 protein (TEX101). Western blots data revealed that bovine sperm TEX101 is present in both testicular and epididymal sperm PM fractions. The native TEX101 polypeptide contains ~17 kDa N-linked oligosaccharides and the polypeptide is anchored to sperm membrane via a glycosylphosphatidylinositol lipid linkage. Immunofluorescence staining of sperm with anti-TEX101 demonstrated that the polypeptide is localized at the head of cauda sperm. Our biochemical results provide evidence on the presence of TEX101 in bovine epididymal sperm plasma membranes and may have a potential role in sperm-egg interaction.
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spelling pubmed-40037722014-05-11 Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa Nagdas, Subir K. McLean, Eric L. Richardson, Leeá P. Raychoudhury, Samir Biochem Res Int Research Article Several studies exhibit the presence of Ricinus Communis Agglutinin I (RCA) binding glycocalyx in mammalian spermatozoa. However, the molecular characterization of RCA binding glycocalyx in sperm membranes and its mechanism of action are poorly understood. The objective of the study was to identify and to characterize RCA binding glycoprotein of the bovine sperm plasma membranes (PM). Lectin blots of caput and cauda sperm PM revealed a 38 kDa polypeptide exhibiting the highest affinity to RCA among the several major RCA binding polypeptides. The 38 kDa RCA binding polypeptide of cauda sperm PM was purified and exhibited a charge train of three distinct spots with isoelectric points (pH 5.3 and 5.8). Proteomic identification yielded ten peptides that matched the sequence of Testis Expressed 101 protein (TEX101). Western blots data revealed that bovine sperm TEX101 is present in both testicular and epididymal sperm PM fractions. The native TEX101 polypeptide contains ~17 kDa N-linked oligosaccharides and the polypeptide is anchored to sperm membrane via a glycosylphosphatidylinositol lipid linkage. Immunofluorescence staining of sperm with anti-TEX101 demonstrated that the polypeptide is localized at the head of cauda sperm. Our biochemical results provide evidence on the presence of TEX101 in bovine epididymal sperm plasma membranes and may have a potential role in sperm-egg interaction. Hindawi Publishing Corporation 2014 2014-04-10 /pmc/articles/PMC4003772/ /pubmed/24818024 http://dx.doi.org/10.1155/2014/573293 Text en Copyright © 2014 Subir K. Nagdas et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nagdas, Subir K.
McLean, Eric L.
Richardson, Leeá P.
Raychoudhury, Samir
Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa
title Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa
title_full Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa
title_fullStr Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa
title_full_unstemmed Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa
title_short Identification and Characterization of TEX101 in Bovine Epididymal Spermatozoa
title_sort identification and characterization of tex101 in bovine epididymal spermatozoa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4003772/
https://www.ncbi.nlm.nih.gov/pubmed/24818024
http://dx.doi.org/10.1155/2014/573293
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