Identification and Preservation of Intestinal Parasites Using Methylene Blue-Glycerol Mount: A New Approach to Stool Microscopy

We have tried a new approach to routine stool microscopy by using a combination of methylene blue and glycerol in wet mount preparation of fresh faecal samples for the demonstration of medically important intestinal parasites. This combination was evaluated for finding differences in the details and...

Descripción completa

Detalles Bibliográficos
Autores principales: Khanna, Vinay, Tilak, Kriti, Rasheed, Shihnin, Mukhopadhyay, Chiranjay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4003778/
https://www.ncbi.nlm.nih.gov/pubmed/24818011
http://dx.doi.org/10.1155/2014/672018
_version_ 1782313884667871232
author Khanna, Vinay
Tilak, Kriti
Rasheed, Shihnin
Mukhopadhyay, Chiranjay
author_facet Khanna, Vinay
Tilak, Kriti
Rasheed, Shihnin
Mukhopadhyay, Chiranjay
author_sort Khanna, Vinay
collection PubMed
description We have tried a new approach to routine stool microscopy by using a combination of methylene blue and glycerol in wet mount preparation of fresh faecal samples for the demonstration of medically important intestinal parasites. This combination was evaluated for finding differences in the details and clarity of morphology and internal structures of parasites under low- and high-power microscopy as compared to iodine and saline mount. It was further evaluated to estimate the time taken by methylene blue-glycerol mount to dry up as compared to iodine and saline wet mount.
format Online
Article
Text
id pubmed-4003778
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-40037782014-05-11 Identification and Preservation of Intestinal Parasites Using Methylene Blue-Glycerol Mount: A New Approach to Stool Microscopy Khanna, Vinay Tilak, Kriti Rasheed, Shihnin Mukhopadhyay, Chiranjay J Parasitol Res Research Article We have tried a new approach to routine stool microscopy by using a combination of methylene blue and glycerol in wet mount preparation of fresh faecal samples for the demonstration of medically important intestinal parasites. This combination was evaluated for finding differences in the details and clarity of morphology and internal structures of parasites under low- and high-power microscopy as compared to iodine and saline mount. It was further evaluated to estimate the time taken by methylene blue-glycerol mount to dry up as compared to iodine and saline wet mount. Hindawi Publishing Corporation 2014 2014-04-10 /pmc/articles/PMC4003778/ /pubmed/24818011 http://dx.doi.org/10.1155/2014/672018 Text en Copyright © 2014 Vinay Khanna et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Khanna, Vinay
Tilak, Kriti
Rasheed, Shihnin
Mukhopadhyay, Chiranjay
Identification and Preservation of Intestinal Parasites Using Methylene Blue-Glycerol Mount: A New Approach to Stool Microscopy
title Identification and Preservation of Intestinal Parasites Using Methylene Blue-Glycerol Mount: A New Approach to Stool Microscopy
title_full Identification and Preservation of Intestinal Parasites Using Methylene Blue-Glycerol Mount: A New Approach to Stool Microscopy
title_fullStr Identification and Preservation of Intestinal Parasites Using Methylene Blue-Glycerol Mount: A New Approach to Stool Microscopy
title_full_unstemmed Identification and Preservation of Intestinal Parasites Using Methylene Blue-Glycerol Mount: A New Approach to Stool Microscopy
title_short Identification and Preservation of Intestinal Parasites Using Methylene Blue-Glycerol Mount: A New Approach to Stool Microscopy
title_sort identification and preservation of intestinal parasites using methylene blue-glycerol mount: a new approach to stool microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4003778/
https://www.ncbi.nlm.nih.gov/pubmed/24818011
http://dx.doi.org/10.1155/2014/672018
work_keys_str_mv AT khannavinay identificationandpreservationofintestinalparasitesusingmethyleneblueglycerolmountanewapproachtostoolmicroscopy
AT tilakkriti identificationandpreservationofintestinalparasitesusingmethyleneblueglycerolmountanewapproachtostoolmicroscopy
AT rasheedshihnin identificationandpreservationofintestinalparasitesusingmethyleneblueglycerolmountanewapproachtostoolmicroscopy
AT mukhopadhyaychiranjay identificationandpreservationofintestinalparasitesusingmethyleneblueglycerolmountanewapproachtostoolmicroscopy

Ejemplares similares