A New Direct Single-Molecule Observation Method for DNA Synthesis Reaction Using Fluorescent Replication Protein A

Using a single-stranded region tracing system, single-molecule DNA synthesis reactions were directly observed in microflow channels. The direct single-molecule observations of DNA synthesis were labeled with a fusion protein consisting of the ssDNA-binding domain of a 70-kDa subunit of replication p...

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Detalles Bibliográficos
Autores principales: Takahashi, Shunsuke, Kawasaki, Shohei, Miyata, Hidefumi, Kurita, Hirofumi, Mizuno, Takeshi, Matsuura, Shun-ichi, Mizuno, Akira, Oshige, Masahiko, Katsura, Shinji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4003986/
https://www.ncbi.nlm.nih.gov/pubmed/24625741
http://dx.doi.org/10.3390/s140305174

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