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Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus

To develop a convenient; sensitive; accurate; and economical technique for genotyping of hepatitis B viruses (HBVs). METHODS: The mercapto-modified B1; B2; C1; and C2-specific genotyping probes consisted of two probes for each HBV genotype that served as a double verification system. These probes we...

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Autores principales: Chen, Jinyuan, Weng, Shaohuang, Chen, Qingqiong, Liu, Ailin, Wang, Fengqing, Chen, Jing, Yi, Qiang, Liu, Qicai, Lin, Xinhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004010/
https://www.ncbi.nlm.nih.gov/pubmed/24658623
http://dx.doi.org/10.3390/s140305611
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author Chen, Jinyuan
Weng, Shaohuang
Chen, Qingqiong
Liu, Ailin
Wang, Fengqing
Chen, Jing
Yi, Qiang
Liu, Qicai
Lin, Xinhua
author_facet Chen, Jinyuan
Weng, Shaohuang
Chen, Qingqiong
Liu, Ailin
Wang, Fengqing
Chen, Jing
Yi, Qiang
Liu, Qicai
Lin, Xinhua
author_sort Chen, Jinyuan
collection PubMed
description To develop a convenient; sensitive; accurate; and economical technique for genotyping of hepatitis B viruses (HBVs). METHODS: The mercapto-modified B1; B2; C1; and C2-specific genotyping probes consisted of two probes for each HBV genotype that served as a double verification system. These probes were fixed on the surface of No. 1; 2; 3; and 4 gold electrodes; respectively; via Au-S bonds. Different charge generated by the binding of RuHex to phosphate groups of the DNA backbone before and after hybridization was used for distinguishing the different genotypes. RESULTS: During hybridization with genotype B; the charges detected at the No. 1 and 2 electrodes were significantly increased; while the charge at the No. 3 and 4 electrodes did not change significantly. During hybridization with genotype C; the charges detected at No. 3 and 4 electrodes were significantly increased; while the signals remained unchanged at the No. 1 and 2 electrodes. During hybridization with mixed genotypes (B and C); the charges detected at all four electrodes were significantly increased. The linear range of detection was 10(−7) to 10(−10) mol/L and the sensitivity for detecting mixed B (10%) or C (10%). CONCLUSIONS: Rapid genotyping of HBVs based on electrochemical sensing is simple, has good specificity; and can greatly reduce the cost. This method can be used for sensitive detection of mixed B and C HBV genotypes.
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spelling pubmed-40040102014-04-29 Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus Chen, Jinyuan Weng, Shaohuang Chen, Qingqiong Liu, Ailin Wang, Fengqing Chen, Jing Yi, Qiang Liu, Qicai Lin, Xinhua Sensors (Basel) Article To develop a convenient; sensitive; accurate; and economical technique for genotyping of hepatitis B viruses (HBVs). METHODS: The mercapto-modified B1; B2; C1; and C2-specific genotyping probes consisted of two probes for each HBV genotype that served as a double verification system. These probes were fixed on the surface of No. 1; 2; 3; and 4 gold electrodes; respectively; via Au-S bonds. Different charge generated by the binding of RuHex to phosphate groups of the DNA backbone before and after hybridization was used for distinguishing the different genotypes. RESULTS: During hybridization with genotype B; the charges detected at the No. 1 and 2 electrodes were significantly increased; while the charge at the No. 3 and 4 electrodes did not change significantly. During hybridization with genotype C; the charges detected at No. 3 and 4 electrodes were significantly increased; while the signals remained unchanged at the No. 1 and 2 electrodes. During hybridization with mixed genotypes (B and C); the charges detected at all four electrodes were significantly increased. The linear range of detection was 10(−7) to 10(−10) mol/L and the sensitivity for detecting mixed B (10%) or C (10%). CONCLUSIONS: Rapid genotyping of HBVs based on electrochemical sensing is simple, has good specificity; and can greatly reduce the cost. This method can be used for sensitive detection of mixed B and C HBV genotypes. MDPI 2014-03-20 /pmc/articles/PMC4004010/ /pubmed/24658623 http://dx.doi.org/10.3390/s140305611 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Chen, Jinyuan
Weng, Shaohuang
Chen, Qingqiong
Liu, Ailin
Wang, Fengqing
Chen, Jing
Yi, Qiang
Liu, Qicai
Lin, Xinhua
Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus
title Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus
title_full Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus
title_fullStr Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus
title_full_unstemmed Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus
title_short Development of an Electrochemical Sensing Technique for Rapid Genotyping of Hepatitis B Virus
title_sort development of an electrochemical sensing technique for rapid genotyping of hepatitis b virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004010/
https://www.ncbi.nlm.nih.gov/pubmed/24658623
http://dx.doi.org/10.3390/s140305611
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