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MicroRNA-224 Suppresses Colorectal Cancer Cell Migration by Targeting Cdc42

The metastatic spread of tumor cells is the major risk factor affecting the clinical prognosis of colorectal cancer (CRC) patients. The metastatic phenotype can be modulated by dysregulating the synthesis of different structural and functional proteins of tumor cells. Micro(mi)RNAs are noncoding RNA...

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Autores principales: Ke, Tao-Wei, Hsu, Han-Lin, Wu, Yu-Hua, Chen, William Tzu-Liang, Cheng, Ya-Wen, Cheng, Chao-Wen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004168/
https://www.ncbi.nlm.nih.gov/pubmed/24817781
http://dx.doi.org/10.1155/2014/617150
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author Ke, Tao-Wei
Hsu, Han-Lin
Wu, Yu-Hua
Chen, William Tzu-Liang
Cheng, Ya-Wen
Cheng, Chao-Wen
author_facet Ke, Tao-Wei
Hsu, Han-Lin
Wu, Yu-Hua
Chen, William Tzu-Liang
Cheng, Ya-Wen
Cheng, Chao-Wen
author_sort Ke, Tao-Wei
collection PubMed
description The metastatic spread of tumor cells is the major risk factor affecting the clinical prognosis of colorectal cancer (CRC) patients. The metastatic phenotype can be modulated by dysregulating the synthesis of different structural and functional proteins of tumor cells. Micro(mi)RNAs are noncoding RNAs that recognize their cognate messenger (m)RNA targets by sequence-specific interactions with the 3′ untranslated region and are involved in the multistep process of CRC development. The objective of this study was to investigate the expression and biological roles of miR-224 in CRC. The miR-224 expression level was assessed by a quantitative real-time PCR in 79 CRC and 18 nontumor tissues. Expression levels of miR-224 in CRC tissues were significantly lower than those in nontumor tissues. Its expression level was associated with the mutation status of the APC gene. Ectopic expression of miR-224 suppressed the migratory ability of CRC cell line, but cell proliferation was less affected. Increased miR-224 diminished Cdc42 and SMAD4 expressions at both the protein and mRNA levels and inhibited the formation of actin filaments. Overall, this study indicated a role of miR-224 in negatively regulating CRC cell migration. The expression level of miR-224 may be a useful predictive biomarker for CRC progression.
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spelling pubmed-40041682014-05-11 MicroRNA-224 Suppresses Colorectal Cancer Cell Migration by Targeting Cdc42 Ke, Tao-Wei Hsu, Han-Lin Wu, Yu-Hua Chen, William Tzu-Liang Cheng, Ya-Wen Cheng, Chao-Wen Dis Markers Research Article The metastatic spread of tumor cells is the major risk factor affecting the clinical prognosis of colorectal cancer (CRC) patients. The metastatic phenotype can be modulated by dysregulating the synthesis of different structural and functional proteins of tumor cells. Micro(mi)RNAs are noncoding RNAs that recognize their cognate messenger (m)RNA targets by sequence-specific interactions with the 3′ untranslated region and are involved in the multistep process of CRC development. The objective of this study was to investigate the expression and biological roles of miR-224 in CRC. The miR-224 expression level was assessed by a quantitative real-time PCR in 79 CRC and 18 nontumor tissues. Expression levels of miR-224 in CRC tissues were significantly lower than those in nontumor tissues. Its expression level was associated with the mutation status of the APC gene. Ectopic expression of miR-224 suppressed the migratory ability of CRC cell line, but cell proliferation was less affected. Increased miR-224 diminished Cdc42 and SMAD4 expressions at both the protein and mRNA levels and inhibited the formation of actin filaments. Overall, this study indicated a role of miR-224 in negatively regulating CRC cell migration. The expression level of miR-224 may be a useful predictive biomarker for CRC progression. Hindawi Publishing Corporation 2014 2014-04-10 /pmc/articles/PMC4004168/ /pubmed/24817781 http://dx.doi.org/10.1155/2014/617150 Text en Copyright © 2014 Tao-Wei Ke et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ke, Tao-Wei
Hsu, Han-Lin
Wu, Yu-Hua
Chen, William Tzu-Liang
Cheng, Ya-Wen
Cheng, Chao-Wen
MicroRNA-224 Suppresses Colorectal Cancer Cell Migration by Targeting Cdc42
title MicroRNA-224 Suppresses Colorectal Cancer Cell Migration by Targeting Cdc42
title_full MicroRNA-224 Suppresses Colorectal Cancer Cell Migration by Targeting Cdc42
title_fullStr MicroRNA-224 Suppresses Colorectal Cancer Cell Migration by Targeting Cdc42
title_full_unstemmed MicroRNA-224 Suppresses Colorectal Cancer Cell Migration by Targeting Cdc42
title_short MicroRNA-224 Suppresses Colorectal Cancer Cell Migration by Targeting Cdc42
title_sort microrna-224 suppresses colorectal cancer cell migration by targeting cdc42
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004168/
https://www.ncbi.nlm.nih.gov/pubmed/24817781
http://dx.doi.org/10.1155/2014/617150
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