Reaction Mechanism of N-Acetylneuraminic Acid Lyase Revealed by a Combination of Crystallography, QM/MM Simulation, and Mutagenesis

[Image: see text] N-Acetylneuraminic acid lyase (NAL) is a Class I aldolase that catalyzes the reversible condensation of pyruvate with N-acetyl-d-mannosamine (ManNAc) to yield the sialic acid N-acetylneuraminic acid (Neu5Ac). Aldolases are finding increasing use as biocatalysts for the stereospecif...

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Detalles Bibliográficos
Autores principales: Daniels, Adam D., Campeotto, Ivan, van der Kamp, Marc W., Bolt, Amanda H., Trinh, Chi H., Phillips, Simon E. V., Pearson, Arwen R., Nelson, Adam, Mulholland, Adrian J., Berry, Alan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004234/
https://www.ncbi.nlm.nih.gov/pubmed/24521460
http://dx.doi.org/10.1021/cb500067z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004234/
https://www.ncbi.nlm.nih.gov/pubmed/24521460
http://dx.doi.org/10.1021/cb500067z

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