Cargando…
QTL architecture of reproductive fitness characters in Brassica rapa
BACKGROUND: Reproductive output is critical to both agronomists seeking to increase seed yield and to evolutionary biologists interested in understanding natural selection. We examine the genetic architecture of diverse reproductive fitness traits in recombinant inbred lines (RILs) developed from a...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004417/ https://www.ncbi.nlm.nih.gov/pubmed/24641198 http://dx.doi.org/10.1186/1471-2229-14-66 |
_version_ | 1782313971644104704 |
---|---|
author | Dechaine, Jennifer M Brock, Marcus T Weinig, Cynthia |
author_facet | Dechaine, Jennifer M Brock, Marcus T Weinig, Cynthia |
author_sort | Dechaine, Jennifer M |
collection | PubMed |
description | BACKGROUND: Reproductive output is critical to both agronomists seeking to increase seed yield and to evolutionary biologists interested in understanding natural selection. We examine the genetic architecture of diverse reproductive fitness traits in recombinant inbred lines (RILs) developed from a crop (seed oil) × wild-like (rapid cycling) genotype of Brassica rapa in field and greenhouse environments. RESULTS: Several fitness traits showed strong correlations and QTL-colocalization across environments (days to bolting, fruit length and seed color). Total fruit number was uncorrelated across environments and most QTL affecting this trait were correspondingly environment-specific. Most fitness components were positively correlated, consistent with life-history theory that genotypic variation in resource acquisition masks tradeoffs. Finally, we detected evidence of transgenerational pleiotropy, that is, maternal days to bolting was negatively correlated with days to offspring germination. A QTL for this transgenerational correlation was mapped to a genomic region harboring one copy of FLOWERING LOCUS C, a genetic locus known to affect both days to flowering as well as germination phenotypes. CONCLUSIONS: This study characterizes the genetic structure of important fitness/yield traits within and between generations in B. rapa. Several identified QTL are suitable candidates for fine-mapping for the improvement of yield in crop Brassicas. Specifically, brFLC1, warrants further investigation as a potential regulator of phenology between generations. |
format | Online Article Text |
id | pubmed-4004417 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-40044172014-04-30 QTL architecture of reproductive fitness characters in Brassica rapa Dechaine, Jennifer M Brock, Marcus T Weinig, Cynthia BMC Plant Biol Research Article BACKGROUND: Reproductive output is critical to both agronomists seeking to increase seed yield and to evolutionary biologists interested in understanding natural selection. We examine the genetic architecture of diverse reproductive fitness traits in recombinant inbred lines (RILs) developed from a crop (seed oil) × wild-like (rapid cycling) genotype of Brassica rapa in field and greenhouse environments. RESULTS: Several fitness traits showed strong correlations and QTL-colocalization across environments (days to bolting, fruit length and seed color). Total fruit number was uncorrelated across environments and most QTL affecting this trait were correspondingly environment-specific. Most fitness components were positively correlated, consistent with life-history theory that genotypic variation in resource acquisition masks tradeoffs. Finally, we detected evidence of transgenerational pleiotropy, that is, maternal days to bolting was negatively correlated with days to offspring germination. A QTL for this transgenerational correlation was mapped to a genomic region harboring one copy of FLOWERING LOCUS C, a genetic locus known to affect both days to flowering as well as germination phenotypes. CONCLUSIONS: This study characterizes the genetic structure of important fitness/yield traits within and between generations in B. rapa. Several identified QTL are suitable candidates for fine-mapping for the improvement of yield in crop Brassicas. Specifically, brFLC1, warrants further investigation as a potential regulator of phenology between generations. BioMed Central 2014-03-18 /pmc/articles/PMC4004417/ /pubmed/24641198 http://dx.doi.org/10.1186/1471-2229-14-66 Text en Copyright © 2014 Dechaine et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. |
spellingShingle | Research Article Dechaine, Jennifer M Brock, Marcus T Weinig, Cynthia QTL architecture of reproductive fitness characters in Brassica rapa |
title | QTL architecture of reproductive fitness characters in Brassica rapa |
title_full | QTL architecture of reproductive fitness characters in Brassica rapa |
title_fullStr | QTL architecture of reproductive fitness characters in Brassica rapa |
title_full_unstemmed | QTL architecture of reproductive fitness characters in Brassica rapa |
title_short | QTL architecture of reproductive fitness characters in Brassica rapa |
title_sort | qtl architecture of reproductive fitness characters in brassica rapa |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4004417/ https://www.ncbi.nlm.nih.gov/pubmed/24641198 http://dx.doi.org/10.1186/1471-2229-14-66 |
work_keys_str_mv | AT dechainejenniferm qtlarchitectureofreproductivefitnesscharactersinbrassicarapa AT brockmarcust qtlarchitectureofreproductivefitnesscharactersinbrassicarapa AT weinigcynthia qtlarchitectureofreproductivefitnesscharactersinbrassicarapa |