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Serum Matrix Metalloproteinase Levels in Patients Exposed to Sulfur Mustard
BACKGROUND: Matrix metalloproteinases (MMPs) are a group of endopeptidases which comprised of various types. These proteolytic enzymes are zinc-dependent and play role in degradation of extracellular matrix (ECM). Various types of cells such as macrophages, fibroblasts, neutrophils, synovial cells a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kowsar
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4005442/ https://www.ncbi.nlm.nih.gov/pubmed/24829780 http://dx.doi.org/10.5812/ircmj.15129 |
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author | Shohrati, Majid Haji Hosseini, Reza Esfandiari, Malek Ashtar Najafian, Nastaran Najafian, Bita Golbedagh, Abbas |
author_facet | Shohrati, Majid Haji Hosseini, Reza Esfandiari, Malek Ashtar Najafian, Nastaran Najafian, Bita Golbedagh, Abbas |
author_sort | Shohrati, Majid |
collection | PubMed |
description | BACKGROUND: Matrix metalloproteinases (MMPs) are a group of endopeptidases which comprised of various types. These proteolytic enzymes are zinc-dependent and play role in degradation of extracellular matrix (ECM). Various types of cells such as macrophages, fibroblasts, neutrophils, synovial cells and some epithelial cells secrete MMPs. According to previous studies on bronchiolitis and respiratory tract lesions in these patients and unknown pathophysiology mechanism up to date, this cross–sectional study was performed. OBJECTIVES: The aim of this study was to compare the serum MMP level in patients with chemical injuries and normal people and also determine the role of these parameters in pulmonary disorders . MATERIALS AND METHODS: In this cross–sectional study, 25 Iranian patients exposed to the sulfur mustard and 25 unexposed participants as the control group were enrolled. Serum samples were collected from two groups and stored at -70˚C until the measurement of MMPs and TIMPs. ELISA kit was used for measurement of MMP and TIMP based on the kit's instruction. For validations in measurement, all samples were analyzed duplicate and in some cases triplicate. RESULTS: The mean level of MMP-9 in serum of chemically-injured group was 1592.42 and this amount in normal group was 679.72 .So there was a significant difference between two groups (P = 0.001) and the mean level of MMP-8 in serum of patients group was 49.10 and in normal group was 35.53. Then there was no significant difference between two groups (P = 0.197). The mean levels of MMP-1 and MMP-2 was not significantly different (P value > 0.05) in the patient and normal groups. And also the mean levels of TIMP-1 and TIMP-2 was not significantly different (P > 0.05) in the patients and normal groups. CONCLUSIONS: In summary, serum MMPs in chemically-injured has shown no significant difference with normal people except for the MMP-9. |
format | Online Article Text |
id | pubmed-4005442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Kowsar |
record_format | MEDLINE/PubMed |
spelling | pubmed-40054422014-05-14 Serum Matrix Metalloproteinase Levels in Patients Exposed to Sulfur Mustard Shohrati, Majid Haji Hosseini, Reza Esfandiari, Malek Ashtar Najafian, Nastaran Najafian, Bita Golbedagh, Abbas Iran Red Crescent Med J Research Article BACKGROUND: Matrix metalloproteinases (MMPs) are a group of endopeptidases which comprised of various types. These proteolytic enzymes are zinc-dependent and play role in degradation of extracellular matrix (ECM). Various types of cells such as macrophages, fibroblasts, neutrophils, synovial cells and some epithelial cells secrete MMPs. According to previous studies on bronchiolitis and respiratory tract lesions in these patients and unknown pathophysiology mechanism up to date, this cross–sectional study was performed. OBJECTIVES: The aim of this study was to compare the serum MMP level in patients with chemical injuries and normal people and also determine the role of these parameters in pulmonary disorders . MATERIALS AND METHODS: In this cross–sectional study, 25 Iranian patients exposed to the sulfur mustard and 25 unexposed participants as the control group were enrolled. Serum samples were collected from two groups and stored at -70˚C until the measurement of MMPs and TIMPs. ELISA kit was used for measurement of MMP and TIMP based on the kit's instruction. For validations in measurement, all samples were analyzed duplicate and in some cases triplicate. RESULTS: The mean level of MMP-9 in serum of chemically-injured group was 1592.42 and this amount in normal group was 679.72 .So there was a significant difference between two groups (P = 0.001) and the mean level of MMP-8 in serum of patients group was 49.10 and in normal group was 35.53. Then there was no significant difference between two groups (P = 0.197). The mean levels of MMP-1 and MMP-2 was not significantly different (P value > 0.05) in the patient and normal groups. And also the mean levels of TIMP-1 and TIMP-2 was not significantly different (P > 0.05) in the patients and normal groups. CONCLUSIONS: In summary, serum MMPs in chemically-injured has shown no significant difference with normal people except for the MMP-9. Kowsar 2014-03-05 2014-03 /pmc/articles/PMC4005442/ /pubmed/24829780 http://dx.doi.org/10.5812/ircmj.15129 Text en Copyright © 2014, Iranian Red Crescent Medical Journal; Published by Kowsar Corp. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Shohrati, Majid Haji Hosseini, Reza Esfandiari, Malek Ashtar Najafian, Nastaran Najafian, Bita Golbedagh, Abbas Serum Matrix Metalloproteinase Levels in Patients Exposed to Sulfur Mustard |
title | Serum Matrix Metalloproteinase Levels in Patients Exposed to Sulfur Mustard |
title_full | Serum Matrix Metalloproteinase Levels in Patients Exposed to Sulfur Mustard |
title_fullStr | Serum Matrix Metalloproteinase Levels in Patients Exposed to Sulfur Mustard |
title_full_unstemmed | Serum Matrix Metalloproteinase Levels in Patients Exposed to Sulfur Mustard |
title_short | Serum Matrix Metalloproteinase Levels in Patients Exposed to Sulfur Mustard |
title_sort | serum matrix metalloproteinase levels in patients exposed to sulfur mustard |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4005442/ https://www.ncbi.nlm.nih.gov/pubmed/24829780 http://dx.doi.org/10.5812/ircmj.15129 |
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