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The use of Mycobacterium tuberculosis HspX and GlcB proteins to identify latent tuberculosis in rheumatoid arthritis patients
Rheumatoid arthritis (RA) is an autoimmune disease characterised by the destruction of articular cartilage and bone damage. The chronic treatment of RA patients causes a higher susceptibility to infectious diseases such as tuberculosis (TB); one-third of the world’s population is latently infected (...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4005526/ https://www.ncbi.nlm.nih.gov/pubmed/24626307 http://dx.doi.org/10.1590/0074-02760140140 |
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author | Silva, Bruna Daniella Souza Tannus-Silva, Daniela Graner Schuwartz Rabahi, Marcelo Fouad Kipnis, Andre Junqueira-Kipnis, Ana Paula |
author_facet | Silva, Bruna Daniella Souza Tannus-Silva, Daniela Graner Schuwartz Rabahi, Marcelo Fouad Kipnis, Andre Junqueira-Kipnis, Ana Paula |
author_sort | Silva, Bruna Daniella Souza |
collection | PubMed |
description | Rheumatoid arthritis (RA) is an autoimmune disease characterised by the destruction of articular cartilage and bone damage. The chronic treatment of RA patients causes a higher susceptibility to infectious diseases such as tuberculosis (TB); one-third of the world’s population is latently infected (LTBI) with Mycobacterium tuberculosis (Mtb). The tuberculin skin test is used to identify individuals LTBI, but many studies have shown that this test is not suitable for RA patients. The goal of this work was to test the specific cellular immune responses to the Mtb malate synthase (GlcB) and heat shock protein X (HspX) antigens of RA patients and to correlate those responses with LTBI status. The T-helper (Th)1, Th17 and Treg-specific immune responses to the GlcB and HspX Mtb antigens were analysed in RA patients candidates for tumour necrosis factor-α blocker treatment. Our results demonstrated that LTBI RA patients had Th1-specific immune responses to GlcB and HspX. Patients were followed up over two years and 14.3% developed active TB. After the development of active TB, RA patients had increased numbers of Th17 and Treg cells, similar to TB patients. These results demonstrate that a GlcB and HspX antigen assay can be used as a diagnostic test to identify LTBI RA patients. |
format | Online Article Text |
id | pubmed-4005526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-40055262014-05-21 The use of Mycobacterium tuberculosis HspX and GlcB proteins to identify latent tuberculosis in rheumatoid arthritis patients Silva, Bruna Daniella Souza Tannus-Silva, Daniela Graner Schuwartz Rabahi, Marcelo Fouad Kipnis, Andre Junqueira-Kipnis, Ana Paula Mem Inst Oswaldo Cruz Articles Rheumatoid arthritis (RA) is an autoimmune disease characterised by the destruction of articular cartilage and bone damage. The chronic treatment of RA patients causes a higher susceptibility to infectious diseases such as tuberculosis (TB); one-third of the world’s population is latently infected (LTBI) with Mycobacterium tuberculosis (Mtb). The tuberculin skin test is used to identify individuals LTBI, but many studies have shown that this test is not suitable for RA patients. The goal of this work was to test the specific cellular immune responses to the Mtb malate synthase (GlcB) and heat shock protein X (HspX) antigens of RA patients and to correlate those responses with LTBI status. The T-helper (Th)1, Th17 and Treg-specific immune responses to the GlcB and HspX Mtb antigens were analysed in RA patients candidates for tumour necrosis factor-α blocker treatment. Our results demonstrated that LTBI RA patients had Th1-specific immune responses to GlcB and HspX. Patients were followed up over two years and 14.3% developed active TB. After the development of active TB, RA patients had increased numbers of Th17 and Treg cells, similar to TB patients. These results demonstrate that a GlcB and HspX antigen assay can be used as a diagnostic test to identify LTBI RA patients. Instituto Oswaldo Cruz, Ministério da Saúde 2013-10-02 2014-02 /pmc/articles/PMC4005526/ /pubmed/24626307 http://dx.doi.org/10.1590/0074-02760140140 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Silva, Bruna Daniella Souza Tannus-Silva, Daniela Graner Schuwartz Rabahi, Marcelo Fouad Kipnis, Andre Junqueira-Kipnis, Ana Paula The use of Mycobacterium tuberculosis HspX and GlcB proteins to identify latent tuberculosis in rheumatoid arthritis patients |
title | The use of Mycobacterium tuberculosis HspX
and GlcB proteins to identify latent tuberculosis in rheumatoid arthritis
patients |
title_full | The use of Mycobacterium tuberculosis HspX
and GlcB proteins to identify latent tuberculosis in rheumatoid arthritis
patients |
title_fullStr | The use of Mycobacterium tuberculosis HspX
and GlcB proteins to identify latent tuberculosis in rheumatoid arthritis
patients |
title_full_unstemmed | The use of Mycobacterium tuberculosis HspX
and GlcB proteins to identify latent tuberculosis in rheumatoid arthritis
patients |
title_short | The use of Mycobacterium tuberculosis HspX
and GlcB proteins to identify latent tuberculosis in rheumatoid arthritis
patients |
title_sort | use of mycobacterium tuberculosis hspx
and glcb proteins to identify latent tuberculosis in rheumatoid arthritis
patients |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4005526/ https://www.ncbi.nlm.nih.gov/pubmed/24626307 http://dx.doi.org/10.1590/0074-02760140140 |
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