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Over-expression of secreted proteins from mammalian cell lines
Secreted mammalian proteins require the development of robust protein over-expression systems for crystallographic and biophysical studies of protein function. Due to complex disulfide bonds and distinct glycosylation patterns preventing folding and expression in prokaryotic expression hosts, many s...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Wiley-Blackwell
2014
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4005704/ https://www.ncbi.nlm.nih.gov/pubmed/24510886 http://dx.doi.org/10.1002/pro.2439 |