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Direct Conversion of Pretreated Straw Cellulose into Citric Acid by Co-cultures of Yarrowia lipolytica SWJ-1b and Immobilized Trichoderma reesei Mycelium
The immobilized cellulase-producing mycelium of Trichoderma reesei was found to produce 2.9 U/ml of cellulase activity within 144 h while 2.1 U/ml of cellulase activity was produced within 120 h by the free mycelium of the same strain. When the immobilized mycelium of T. reesei was co-cultivated wit...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007025/ https://www.ncbi.nlm.nih.gov/pubmed/24659047 http://dx.doi.org/10.1007/s12010-014-0856-8 |
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author | Liu, Xiaoyan Lv, Jinshun Zhang, Tong Deng, Yuanfang |
author_facet | Liu, Xiaoyan Lv, Jinshun Zhang, Tong Deng, Yuanfang |
author_sort | Liu, Xiaoyan |
collection | PubMed |
description | The immobilized cellulase-producing mycelium of Trichoderma reesei was found to produce 2.9 U/ml of cellulase activity within 144 h while 2.1 U/ml of cellulase activity was produced within 120 h by the free mycelium of the same strain. When the immobilized mycelium of T. reesei was co-cultivated with the free cells of Yarrowia lipolytica SWJ-1b in flask, Y. lipolytica SWJ-1b could yield 10.7 g/l of citric acid and 3.9 g/l of isocitric acid from 40.0 g/l pretreated straw within 240 h. Under the similar conditions, Y. lipolytica SWJ-1b could yield 32.8 g/l of citric acid and 4.7 g/l of isocitric acid from 40.0 g/l pretreated straw supplemented with 20.0 g/l glucose within 288 h. When the co-cultures were grown in 10-l fermentor, Y. lipolytica SWJ-1b could yield 83.4 g/l of citric acid and 8.7 g/l of isocitric acid from 100.0 g/l of pretreated straw supplemented with 50.0 g/l glucose within 312 h. |
format | Online Article Text |
id | pubmed-4007025 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-40070252014-05-07 Direct Conversion of Pretreated Straw Cellulose into Citric Acid by Co-cultures of Yarrowia lipolytica SWJ-1b and Immobilized Trichoderma reesei Mycelium Liu, Xiaoyan Lv, Jinshun Zhang, Tong Deng, Yuanfang Appl Biochem Biotechnol Article The immobilized cellulase-producing mycelium of Trichoderma reesei was found to produce 2.9 U/ml of cellulase activity within 144 h while 2.1 U/ml of cellulase activity was produced within 120 h by the free mycelium of the same strain. When the immobilized mycelium of T. reesei was co-cultivated with the free cells of Yarrowia lipolytica SWJ-1b in flask, Y. lipolytica SWJ-1b could yield 10.7 g/l of citric acid and 3.9 g/l of isocitric acid from 40.0 g/l pretreated straw within 240 h. Under the similar conditions, Y. lipolytica SWJ-1b could yield 32.8 g/l of citric acid and 4.7 g/l of isocitric acid from 40.0 g/l pretreated straw supplemented with 20.0 g/l glucose within 288 h. When the co-cultures were grown in 10-l fermentor, Y. lipolytica SWJ-1b could yield 83.4 g/l of citric acid and 8.7 g/l of isocitric acid from 100.0 g/l of pretreated straw supplemented with 50.0 g/l glucose within 312 h. Springer US 2014-03-23 2014 /pmc/articles/PMC4007025/ /pubmed/24659047 http://dx.doi.org/10.1007/s12010-014-0856-8 Text en © Springer Science+Business Media New York 2014 |
spellingShingle | Article Liu, Xiaoyan Lv, Jinshun Zhang, Tong Deng, Yuanfang Direct Conversion of Pretreated Straw Cellulose into Citric Acid by Co-cultures of Yarrowia lipolytica SWJ-1b and Immobilized Trichoderma reesei Mycelium |
title | Direct Conversion of Pretreated Straw Cellulose into Citric Acid by Co-cultures of Yarrowia lipolytica SWJ-1b and Immobilized Trichoderma reesei Mycelium |
title_full | Direct Conversion of Pretreated Straw Cellulose into Citric Acid by Co-cultures of Yarrowia lipolytica SWJ-1b and Immobilized Trichoderma reesei Mycelium |
title_fullStr | Direct Conversion of Pretreated Straw Cellulose into Citric Acid by Co-cultures of Yarrowia lipolytica SWJ-1b and Immobilized Trichoderma reesei Mycelium |
title_full_unstemmed | Direct Conversion of Pretreated Straw Cellulose into Citric Acid by Co-cultures of Yarrowia lipolytica SWJ-1b and Immobilized Trichoderma reesei Mycelium |
title_short | Direct Conversion of Pretreated Straw Cellulose into Citric Acid by Co-cultures of Yarrowia lipolytica SWJ-1b and Immobilized Trichoderma reesei Mycelium |
title_sort | direct conversion of pretreated straw cellulose into citric acid by co-cultures of yarrowia lipolytica swj-1b and immobilized trichoderma reesei mycelium |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007025/ https://www.ncbi.nlm.nih.gov/pubmed/24659047 http://dx.doi.org/10.1007/s12010-014-0856-8 |
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