Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas

BACKGROUND: Kinase module of Mediator complex (‘CDK8 submodule') consists of four subunits: CDK8, Cyclin C, MED12, and MED13. Recently, we reported recurrent MED12 mutations in 70% of uterine leiomyomas. The aim of this study was to analyse whether mutations in other components of the module co...

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Autores principales: Mäkinen, N, Heinonen, H-R, Sjöberg, J, Taipale, J, Vahteristo, P, Aaltonen, L A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007231/
https://www.ncbi.nlm.nih.gov/pubmed/24642626
http://dx.doi.org/10.1038/bjc.2014.138
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author Mäkinen, N
Heinonen, H-R
Sjöberg, J
Taipale, J
Vahteristo, P
Aaltonen, L A
author_facet Mäkinen, N
Heinonen, H-R
Sjöberg, J
Taipale, J
Vahteristo, P
Aaltonen, L A
author_sort Mäkinen, N
collection PubMed
description BACKGROUND: Kinase module of Mediator complex (‘CDK8 submodule') consists of four subunits: CDK8, Cyclin C, MED12, and MED13. Recently, we reported recurrent MED12 mutations in 70% of uterine leiomyomas. The aim of this study was to analyse whether mutations in other components of the module contribute to the development of these lesions. METHODS: Mutation screening of altogether 70 MED12 mutation-negative uterine leiomyomas was carried out by direct sequencing. RESULTS: None of the tumours displayed somatic mutations in the coding regions of CDK8/CDK19, CCNC, or MED13. CONCLUSIONS: Mutations in CDK8/CDK19, CCNC, and MED13 do not frequently contribute to genesis of uterine leiomyomas.
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spelling pubmed-40072312015-04-29 Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas Mäkinen, N Heinonen, H-R Sjöberg, J Taipale, J Vahteristo, P Aaltonen, L A Br J Cancer Short Communication BACKGROUND: Kinase module of Mediator complex (‘CDK8 submodule') consists of four subunits: CDK8, Cyclin C, MED12, and MED13. Recently, we reported recurrent MED12 mutations in 70% of uterine leiomyomas. The aim of this study was to analyse whether mutations in other components of the module contribute to the development of these lesions. METHODS: Mutation screening of altogether 70 MED12 mutation-negative uterine leiomyomas was carried out by direct sequencing. RESULTS: None of the tumours displayed somatic mutations in the coding regions of CDK8/CDK19, CCNC, or MED13. CONCLUSIONS: Mutations in CDK8/CDK19, CCNC, and MED13 do not frequently contribute to genesis of uterine leiomyomas. Nature Publishing Group 2014-04-29 2014-03-18 /pmc/articles/PMC4007231/ /pubmed/24642626 http://dx.doi.org/10.1038/bjc.2014.138 Text en Copyright © 2014 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/3.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Short Communication
Mäkinen, N
Heinonen, H-R
Sjöberg, J
Taipale, J
Vahteristo, P
Aaltonen, L A
Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas
title Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas
title_full Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas
title_fullStr Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas
title_full_unstemmed Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas
title_short Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas
title_sort mutation analysis of components of the mediator kinase module in med12 mutation-negative uterine leiomyomas
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007231/
https://www.ncbi.nlm.nih.gov/pubmed/24642626
http://dx.doi.org/10.1038/bjc.2014.138
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