Negative Subtraction Hybridization: An efficient method to isolate large numbers of condition-specific cDNAs

BACKGROUND: The construction of cDNA libraries is a useful tool to understand gene expression in organisms under different conditions, but random sequencing of unbiased cDNA collections is laborious and can give rise to redundant EST collections. We aimed to isolate cDNAs of messages induced by swit...

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Detalles Bibliográficos
Autores principales: Ray, Anamika, Macwana, Sunita, Ayoubi, Patricia, Hall, Leo T, Prade, Rolf, Mort, Andrew J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC400731/
https://www.ncbi.nlm.nih.gov/pubmed/15050035
http://dx.doi.org/10.1186/1471-2164-5-22

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC400731/
https://www.ncbi.nlm.nih.gov/pubmed/15050035
http://dx.doi.org/10.1186/1471-2164-5-22

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