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Integrated transcriptomics and metabolomics decipher differences in the resistance of pedunculate oak to the herbivore Tortrix viridana L.

BACKGROUND: The interaction between insect pests and their host plants is a never-ending race of evolutionary adaption. Plants have developed an armament against insect herbivore attacks, and attackers continuously learn how to address it. Using a combined transcriptomic and metabolomic approach, we...

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Autores principales: Kersten, Birgit, Ghirardo, Andrea, Schnitzler, Jörg-Peter, Kanawati, Basem, Schmitt-Kopplin, Philippe, Fladung, Matthias, Schroeder, Hilke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007517/
https://www.ncbi.nlm.nih.gov/pubmed/24160444
http://dx.doi.org/10.1186/1471-2164-14-737
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author Kersten, Birgit
Ghirardo, Andrea
Schnitzler, Jörg-Peter
Kanawati, Basem
Schmitt-Kopplin, Philippe
Fladung, Matthias
Schroeder, Hilke
author_facet Kersten, Birgit
Ghirardo, Andrea
Schnitzler, Jörg-Peter
Kanawati, Basem
Schmitt-Kopplin, Philippe
Fladung, Matthias
Schroeder, Hilke
author_sort Kersten, Birgit
collection PubMed
description BACKGROUND: The interaction between insect pests and their host plants is a never-ending race of evolutionary adaption. Plants have developed an armament against insect herbivore attacks, and attackers continuously learn how to address it. Using a combined transcriptomic and metabolomic approach, we investigated the molecular and biochemical differences between Quercus robur L. trees that resisted (defined as resistant oak type) or were susceptible (defined as susceptible oak type) to infestation by the major oak pest, Tortrix viridana L. RESULTS: Next generation RNA sequencing revealed hundreds of genes that exhibited constitutive and/or inducible differential expression in the resistant oak compared to the susceptible oak. Distinct differences were found in the transcript levels and the metabolic content with regard to tannins, flavonoids, and terpenoids, which are compounds involved in the defence against insect pests. The results of our transcriptomic and metabolomic analyses are in agreement with those of a previous study in which we showed that female moths prefer susceptible oaks due to their specific profile of herbivore-induced volatiles. These data therefore define two oak genotypes that clearly differ on the transcriptomic and metabolomic levels, as reflected by their specific defensive compound profiles. CONCLUSIONS: We conclude that the resistant oak type seem to prefer a strategy of constitutive defence responses in contrast to more induced defence responses of the susceptible oaks triggered by feeding. These results pave the way for the development of biomarkers for an early determination of potentially green oak leaf roller-resistant genotypes in natural pedunculate oak populations in Europe.
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spelling pubmed-40075172014-05-03 Integrated transcriptomics and metabolomics decipher differences in the resistance of pedunculate oak to the herbivore Tortrix viridana L. Kersten, Birgit Ghirardo, Andrea Schnitzler, Jörg-Peter Kanawati, Basem Schmitt-Kopplin, Philippe Fladung, Matthias Schroeder, Hilke BMC Genomics Research Article BACKGROUND: The interaction between insect pests and their host plants is a never-ending race of evolutionary adaption. Plants have developed an armament against insect herbivore attacks, and attackers continuously learn how to address it. Using a combined transcriptomic and metabolomic approach, we investigated the molecular and biochemical differences between Quercus robur L. trees that resisted (defined as resistant oak type) or were susceptible (defined as susceptible oak type) to infestation by the major oak pest, Tortrix viridana L. RESULTS: Next generation RNA sequencing revealed hundreds of genes that exhibited constitutive and/or inducible differential expression in the resistant oak compared to the susceptible oak. Distinct differences were found in the transcript levels and the metabolic content with regard to tannins, flavonoids, and terpenoids, which are compounds involved in the defence against insect pests. The results of our transcriptomic and metabolomic analyses are in agreement with those of a previous study in which we showed that female moths prefer susceptible oaks due to their specific profile of herbivore-induced volatiles. These data therefore define two oak genotypes that clearly differ on the transcriptomic and metabolomic levels, as reflected by their specific defensive compound profiles. CONCLUSIONS: We conclude that the resistant oak type seem to prefer a strategy of constitutive defence responses in contrast to more induced defence responses of the susceptible oaks triggered by feeding. These results pave the way for the development of biomarkers for an early determination of potentially green oak leaf roller-resistant genotypes in natural pedunculate oak populations in Europe. BioMed Central 2013-10-28 /pmc/articles/PMC4007517/ /pubmed/24160444 http://dx.doi.org/10.1186/1471-2164-14-737 Text en Copyright © 2013 Kersten et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kersten, Birgit
Ghirardo, Andrea
Schnitzler, Jörg-Peter
Kanawati, Basem
Schmitt-Kopplin, Philippe
Fladung, Matthias
Schroeder, Hilke
Integrated transcriptomics and metabolomics decipher differences in the resistance of pedunculate oak to the herbivore Tortrix viridana L.
title Integrated transcriptomics and metabolomics decipher differences in the resistance of pedunculate oak to the herbivore Tortrix viridana L.
title_full Integrated transcriptomics and metabolomics decipher differences in the resistance of pedunculate oak to the herbivore Tortrix viridana L.
title_fullStr Integrated transcriptomics and metabolomics decipher differences in the resistance of pedunculate oak to the herbivore Tortrix viridana L.
title_full_unstemmed Integrated transcriptomics and metabolomics decipher differences in the resistance of pedunculate oak to the herbivore Tortrix viridana L.
title_short Integrated transcriptomics and metabolomics decipher differences in the resistance of pedunculate oak to the herbivore Tortrix viridana L.
title_sort integrated transcriptomics and metabolomics decipher differences in the resistance of pedunculate oak to the herbivore tortrix viridana l.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4007517/
https://www.ncbi.nlm.nih.gov/pubmed/24160444
http://dx.doi.org/10.1186/1471-2164-14-737
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